2005
DOI: 10.11150/kansenshogakuzasshi1970.79.129
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Molecular Epidemiology of Mechicillin-resistantStaphylococcus aureus(MRSA) by Pulsed-Field Gel Electrophoresis

Abstract: A total of 103 methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients and 78 MRSA from hospital workers during the years 1990 to 1994, and 52 MRSA from patients in year 2000, in one hospital at Wakayama Prefecture, were typed by pulsed-field gel electrophoresis (PFGE) profiles. MRSA isolates were grouped into 20 genomic types. These types were further divided into 57 subtypes. The type 1 was dominant among patients, and closely related type 1 strains were spread during 1990-1993. In contrast… Show more

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“…A methicillin-resistant Staphylococcus aureus (MRSA) strain isolated from a patient in a hospital at Wakayama was kindly provided by Dr. Tanaka (Sakai City Institute of Public Health, Osaka). The strain (10 5 cell/0.5 mL in 0.9% NaCl solution) was streaked onto the surface of agar (Nissui Tube Sulfide Indole Motility Medium, Nippon Suisan Kaisha, Ltd., Tokyo, Japan).…”
Section: Methodsmentioning
confidence: 99%
“…A methicillin-resistant Staphylococcus aureus (MRSA) strain isolated from a patient in a hospital at Wakayama was kindly provided by Dr. Tanaka (Sakai City Institute of Public Health, Osaka). The strain (10 5 cell/0.5 mL in 0.9% NaCl solution) was streaked onto the surface of agar (Nissui Tube Sulfide Indole Motility Medium, Nippon Suisan Kaisha, Ltd., Tokyo, Japan).…”
Section: Methodsmentioning
confidence: 99%