Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from pigs in Japan

Ozawa, Manao; Furuya, Yukari; Akama, Ryoko; Harada, Saki; Matsuda, Manabu; Abo, Hitoshi; Shirakawa, Takahiro; Kawanishi, Michihiro; Yoshida, Eiji; Furuno, Minako; Fukuhara, Hisae; Kasuya, Kazufumi; Shimazaki, Yohko

doi:10.1016/j.vetmic.2022.109523

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…In the report, the pig-derived isolate was characterized by erm(C) and/or vag(A) and tet(T)/tet(L), and the human-derived isolate was characterized by erm(A) and had no tet(T)/tet(L) or vag(A) gene. In this study, ST5 isolates had only erm(C) and not erm(A), and had tet(L) and vag(A) genes, similar to the ST5 isolate from swine in the USA and a previous Japanese report [19,40]. Furthermore, our SNP analysis revealed that the Japanese human-origin ST5 strain Mu50 was located in a different cluster from the pig-origin isolate ST5.…”

Section: Discussionsupporting

confidence: 75%

Prevalence and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Pigs in Japan

Kawanishi,

Matsuda,

Abo

et al. 2024

Antibiotics

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We investigated the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in pig slaughterhouses from 2018 to 2022 in Japan and the isolates were examined for antimicrobial susceptibility and genetic characteristics by whole-genome analysis. Although the positive LA-MRSA rates on farms (29.6%) and samples (9.9%) in 2022 in Japan remained lower than those observed in European countries exhibiting extremely high rates of confirmed human LA-MRSA infections, these rates showed a gradually increasing trend over five years. The ST398/t034 strain was predominant, followed by ST5/t002, and differences were identified between ST398 and ST5 in terms of antimicrobial susceptibility and the resistance genes carried. Notably, LA-MRSA possessed resistance genes toward many antimicrobial classes, with 91.4% of the ST398 strains harboring zinc resistance genes. These findings indicate that the co-selection pressure associated with multidrug and zinc resistance may have contributed markedly to LA-MRSA persistence. SNP analysis revealed that ST398 and ST5 of swine origin were classified into a different cluster of MRSA from humans, showing the same ST in Japan and lacking the immune evasion genes (scn, sak, or chp). Although swine-origin LA-MRSA is currently unlikely to spread to humans and become a problem in current clinical practice, preventing its dissemination requires using antimicrobials prudently, limiting zinc utilization to the minimum required nutrient, and practicing fundamental hygiene measures.

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Section: Discussionsupporting

confidence: 75%

Prevalence and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Pigs in Japan

Kawanishi,

Matsuda,

Abo

et al. 2024

Antibiotics

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“…The prevalence of MRSA in pig production was diverse in Western countries, with 77% in the US, 80% in Denmark, 69.7% in Belgium, and 99.5% in the Netherlands (Crombé et al, 2012;Sun et al, 2015;Dierikx et al, 2016;Schulz et al, 2019). In Japan, MRSA was detected among pigs in slaughterhouses, diseased pigs on farms, imported breeding pigs, and farm dust, with a prevalence of 5.2, 3.4, 28.8, and 0.06%, respectively (Ozawa et al, 2022). This discrepancy in MRSA in pig production suggests the diversity of MRSA distribution across different geographical locations.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of Staphylococcus aureus and the emergence of livestock-associated MRSA CC398 in pig production in eastern China

Zheng,

Jiang,

Wang

et al. 2023

Front. Microbiol.

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Livestock-associated Staphylococcus aureus (LA-MRSA) has been of increasing concern due to its potential risk to humans. This study investigated the prevalence of MRSA in pig production in Eastern China and determined the genomic characteristics of pig-associated MRSA isolates by whole-genome sequencing (WGS). A total of 1,318 samples were collected from pig farms and pig slaughterhouses, and 150 S. aureus were identified, including 63 MRSA isolates and 87 MSSA isolates. MRSA was detected in all pig farms and pig slaughterhouses. The antimicrobial susceptibility test revealed that all MRSA isolates were multidrug-resistant. The WGS and MLST analysis demonstrated that 56 MRSA isolates belonged to clonal complex (CC) 398, and seven MRSA isolates belonged to CC9. All LA-MRSA isolates were absent of phiSa3 phage containing immune evasion cluster (IEC) and possessed an intact hlb gene. In addition, genes associated with Panton-Valentine leukocidin, typically indicative of human adaptation, were not detected. The analysis of antibiotic resistance genes (ARGs) demonstrated that all MRSA isolates contained multiple ARGs. All MRSA isolates had Plthe mecA gene and at least one tetracycline resistance gene. Both tetM and tetK were detected in all MRSA CC398 isolates, while tetL was detected in all MRSA CC9 isolates. The phenicol resistance gene fexA was detected in 51 MRSA isolates, while the linezolid resistance gene cfr was detected in 60 MRSA isolates. The emergence of LA-MRSA CC398 in four pig farms and one slaughterhouse in this study indicates the spread of this clonal complex in the pig production sector in Eastern China. Further investigations are required to understand the potential transmission routes of LA-MRSA CC398 within the pork production chain in China and to assess the potential risks to humans.

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Monitoring longitudinal antimicrobial resistance trends of Staphylococcus aureus strains worldwide over the past 100 years to decipher its evolution and transmission

Fang,

Wu,

et al. 2024

Journal of Hazardous Materials

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Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolated from pigs in Japan

Cited by 5 publications

References 24 publications

Prevalence and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Pigs in Japan

Prevalence and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Pigs in Japan

The prevalence of Staphylococcus aureus and the emergence of livestock-associated MRSA CC398 in pig production in eastern China

Monitoring longitudinal antimicrobial resistance trends of Staphylococcus aureus strains worldwide over the past 100 years to decipher its evolution and transmission

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