Molecular epidemiology of multi- and extensively-drug-resistant Mycobacterium tuberculosis in Ireland, 2001–2014

Roycroft, Emma; O’Toole, Ronan; Fitzgibbon, Margaret; Montgomery, Lorraine; O'Meara, Mary; Downes, Phil; Jackson, Sarah; O’Donnell, Joan; Laurenson, Ian F.; McLaughlin, Anne-Marie; Keane, Joseph; Rogers, Thomas R.

doi:10.1016/j.jinf.2017.10.002

Cited by 13 publications

(13 citation statements)

References 45 publications

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“…According to the European Centre for Disease Control (ECDC) surveillance report, Beijing was the most common genotypic lineage among MDR-TB strains isolated in 2015 in Europe (60.0%), followed by LAM (17.6%) [29], despite LAM being more prevalent in sensitive strains (as in Ireland [30] and Belgium [31], for example). By contrast, here, the most common genotypic lineage in MDR-TB was LAM (69%), followed by Beijing (15%) and Haarlem (12%), similarly to a study on sensitive strain in Northern Portugal (LAM represented 61.1%) [18].…”

Section: Discussionmentioning

confidence: 99%

A nationwide study of multidrug-resistant tuberculosis in Portugal 2014–2017 using epidemiological and molecular clustering analyses

et al. 2019

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Background Increasing multidrug-resistant tuberculosis (MDR-TB) incidence is a major threat against TB eradication worldwide. We aim to conduct a detailed MDR-TB study in Portugal, an European country with endemic TB, combining genetic analysis and epidemiological data, in order to assess the efficiency of public health containment of MRD-TB in the country. Methods We used published MIRU-VNTR data, that we reanalysed using a phylogenetic analysis to better describe MDR-TB cases transmission occurring in Portugal from 2014 to 2017, further enriched with epidemiological data of these cases. Results We show an MDR-TB transmission scenario, where MDR strains likely arose and are transmitted within local chains. 63% of strains were clustered, suggesting high primary transmission (estimated as 50% using MIRU-VNTR data and 15% considering epidemiological links). These values are higher than those observed across Europe and even for sensitive strains in Portugal using similar methodologies. MDR-TB cases are associated with individuals born in Portugal and evolutionary analysis suggests a local evolution of strains. Consistently the sublineage LAM, the most common in sensitive strains in Europe, is the more frequent in Portugal in contrast with the remaining European MDR-TB picture where immigrant-associated Beijing strains are more common. Conclusions Despite efforts to track and contain MDR-TB strains in Portugal, their transmission patterns are still as uncontrolled as that of sensitive strains, stressing the need to reinforce surveillance and containment strategies. Electronic supplementary material The online version of this article (10.1186/s12879-019-4189-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

A nationwide study of multidrug-resistant tuberculosis in Portugal 2014–2017 using epidemiological and molecular clustering analyses

et al. 2019

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“…A study [34] on strain diversity and phylogeography using genomes of 340 M. tuberculosis strains (isolated during 2008–2013) from KwaZulu-Natal, helped to elucidate the timing of acquisitions of drug resistance mutations that confer XDR-TB, indicating that isoniazid-resistance evolved earlier than rifampicin-resistance . Similarly, molecular epidemiology of MDR-TB in Ireland has been systematically examined using genomes of M. tuberculosis strains isolated during 2001–2014 from MDR-TB cases [35]. Among seven lineages of MTB complex, Beijing lineage was observed to be associated with MDR [35].…”

Section: Availability Of Genome Sequences and Annotation Data: Applicmentioning

confidence: 99%

“…Similarly, molecular epidemiology of MDR-TB in Ireland has been systematically examined using genomes of M. tuberculosis strains isolated during 2001–2014 from MDR-TB cases [35]. Among seven lineages of MTB complex, Beijing lineage was observed to be associated with MDR [35]. MDR-TB in Ireland was found to be introduced from other localities, as known for several European countries [37].…”

Section: Availability Of Genome Sequences and Annotation Data: Applicmentioning

confidence: 99%

Mycobacterial genomics and structural bioinformatics: opportunities and challenges in drug discovery

Waman

Vedithi

Thomas

et al. 2019

Emerging Microbes & Infections

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Of the more than 190 distinct species of Mycobacterium genus, many are economically and clinically important pathogens of humans or animals. Among those mycobacteria that infect humans, three species namely Mycobacterium tuberculosis (causative agent of tuberculosis), Mycobacterium leprae (causative agent of leprosy) and Mycobacterium abscessus (causative agent of chronic pulmonary infections) pose concern to global public health. Although antibiotics have been successfully developed to combat each of these, the emergence of drug-resistant strains is an increasing challenge for treatment and drug discovery. Here we describe the impact of the rapid expansion of genome sequencing and genome/pathway annotations that have greatly improved the progress of structure-guided drug discovery. We focus on the applications of comparative genomics, metabolomics, evolutionary bioinformatics and structural proteomics to identify potential drug targets. The opportunities and challenges for the design of drugs for M. tuberculosis, M. leprae and M. abscessus to combat resistance are discussed.

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“…There is 7 major M. tuberculosis lineage associated with specific geographic regions, that genetic differences between them affect the distribution and prevalence in different regions ( 2 , 3 ). In addition, the pathogenicity, geographical distribution, antibiotic resistance and lack of response to treatment are also different ( 4 – 6 ). Determination of these genotypes can be an important method of understanding pathogenicity and control of tuberculosis in each region.…”

Section: Introductionmentioning

confidence: 99%

Comparison of two molecular diagnostic methods for identifying Beijing genotype of Mycobacterium tuberculosis

Mahghani¹,

Kargar²,

Kafilzadeh³

et al. 2020

IJM

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Background and Objectives: The Beijing family of Mycobacterium tuberculosis has been identified as a severe pathogen among this species and found in many clinical isolates during the last decade. Early identification of such genotype is import- ant for better prevention and treatment of tuberculosis. The present study performed to compare the efficiency of Real-Time PCR and IS6110-Based Inverse PCR methods to identify the Beijing family. Materials and Methods: This study was carried out on 173 clinical isolates of Mycobacterium tuberculosis complex in Golestan Province, northern Iran. DNA extraction performed by boiling and determining the Beijing and non-Beijing strains carried out using Real-Time PCR and IS6110-Based Inverse PCR. Results: In both Real-Time PCR and IS6110-Based Inverse PCR method, 24 specimens (13.9%) of the Beijing family were identified and the result of the IS6110-Based Inverse PCR method showed that all the Beijing strains in this region belonged to the Ancient Beijing sub-lineage. Conclusion: Although the efficacy of the two methods in the diagnosis of the Beijing family is similar, the IS6110-Based Inverse PCR is more applicable to the ability to detect new and old Beijing family.

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Molecular epidemiology of multi- and extensively-drug-resistant Mycobacterium tuberculosis in Ireland, 2001–2014

Cited by 13 publications

References 45 publications

A nationwide study of multidrug-resistant tuberculosis in Portugal 2014–2017 using epidemiological and molecular clustering analyses

A nationwide study of multidrug-resistant tuberculosis in Portugal 2014–2017 using epidemiological and molecular clustering analyses

Mycobacterial genomics and structural bioinformatics: opportunities and challenges in drug discovery

Comparison of two molecular diagnostic methods for identifying Beijing genotype of Mycobacterium tuberculosis

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