Molecular Epidemiology of Multi-Drug Resistant Acinetobacter baumannii Isolated in Shandong, China

Jiang, Meijie; Liu, Lijuan; Ma, Yonghui; Zhang, Zhijun; Li, Ning; Zhang, Fusen; Zhao, Sheng

doi:10.3389/fmicb.2016.01687

Cited by 21 publications

(23 citation statements)

References 35 publications

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“…Carbapenem resistance in A.baumannii has been reported in various studies, ranging from 40-98.7% (5, [21][22][23][24]. We found 86.9% carbapenem resistance in A. baumannii which is similar to 85% resistance reported by Rynga D et al (2015) from Delhi (25).…”

Section: Discussionsupporting

confidence: 81%

Molecular Epidemiology of Metallo Beta Lactamases in Acinetobacter Baumannii at a Tertiary Care Hospital

Mohammed¹,

Singh²

2019

IJMBS

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Background: Carbapenem resistance mediated by metallo beta lactamases (MBL) in Acinetobacter baumannii is a global challenge due to its rapid spread and limited therapeutic options. Objective: To determine the prevalence of MBL in A. baumannii isolates in hospitalized patients by both phenotypic and genotypic methods. Materials and Methods: The clinical samples were collected from inpatients and subcultured on routine culture media for growth. Identification of bacteria along with antimicrobial sensitivity testing was done by VITEK -2 Compact (bioMerieux). Antibiotics that were not tested by VITEK-2 were tested manually by Kirby-Bauer disk diffusion method according to CLSI 2017 and EUCAST 2016 guidelines. The isolates which were resistant to carbapenem (imipenem and/ or meropenem) were tested by phenotypic (imipenem-EDTA combined disk method) and genotypic method for presence of common metallo beta lactamases genes (blaIMP, blaNDM, blaGIM, blaVIM, blaSPM and blaSIM). Results: 84 non duplicate A.baumannii were isolated out of 947 pathogenic gram negative isolates. Majority (47.6%) of isolates were obtained from tracheostomy/endotracheal/bronchoalveolar lavage (TT/ET/BAL) followed by sputum (21.4%). None of the isolates were found to be resistant to colistin and tigecycline. 73 (86.9%) isolates were found to be carbapenem resistant, among these 60 (82.2%) were found to be MBL positive by phenotypic and 32 (43.2%) by genotypic method. MBL genes detected were blaNDM (39.7%), blaGIM (2.7%) and blaVIM (1.4%). None of the isolates were positive for blaIMP, blaSPM and blaSIM. Conclusion: The prevalence of MBL in carbapenem resistant isolates of A.baumannii was 87.7%. blaNDM was the most common gene detected. No significant difference was found in the ability of phenotypic and genotypic methods for MBL detection. The resistance rate of the A.baumannii is high for most antibiotics except for polymyxins (E&B) and tigecycline. Key words: Metallo beta Lactamases, Acinetobacter baumannii, Carbapenem.

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Section: Discussionsupporting

confidence: 81%

Molecular Epidemiology of Metallo Beta Lactamases in Acinetobacter Baumannii at a Tertiary Care Hospital

Mohammed¹,

Singh²

2019

IJMBS

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“…BSIs caused by A. baumannii strains mostly occurred in ICUs rather than on GWs [ 32 ]. The likely reason is that the A. baumannii BSIs originated predominantly from pulmonary infections associated with ventilators.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of major clinical pathogens in South Korea, May 2016 to April 2017: first one-year report from Kor-GLASS

et al. 2018

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The Korean government established an antimicrobial resistance (AMR) surveillance system, compatible with the Global AMR Surveillance System (GLASS): Kor-GLASS. We describe results from the first year of operation of the Kor-GLASS from May 2016 to April 2017, comprising all non-duplicated clinical isolates of major pathogens from blood, urine, faeces and urethral and cervical swabs from six sentinel hospitals. Antimicrobial susceptibility tests were carried out by disk diffusion, Etest, broth microdilution and agar dilution methods. Among 67,803 blood cultures, 3,523 target pathogens were recovered. The predominant bacterial species were Escherichia coli (n = 1,536), Klebsiella pneumoniae (n = 597) and Staphylococcus aureus (n = 584). From 57,477 urine cultures, 6,394 E. coli and 1,097 K. pneumoniae were recovered. Bloodstream infections in inpatients per 10,000 patient-days (10TPD) were highest for cefotaxime-resistant E. coli with 2.1, followed by 1.6 for meticillin-resistant Sta. aureus, 1.1 for imipenem-resistant Acinetobacter baumannii, 0.8 for cefotaxime-resistant K. pneumoniae and 0.4 for vancomycin-resistant Enterococcus faecium. Urinary tract infections in inpatients were 7.7 and 2.1 per 10TPD for cefotaxime-resistant E. coli and K. pneumoniae, respectively. Kor-GLASS generated well-curated surveillance data devoid of collection bias or isolate duplication. A bacterial bank and a database for the collections are under development.

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“…Despite extensive research on the epidemiology and population evolution of A. baumannii worldwide, the understanding of the evolutionary processes driving A. baumannii multidrug resistance and genome diversity in China remains incomplete, especially in the central and western regions of the country. Only in the eastern and coastal areas has a comprehensive genetic study of A. baumannii collected at different sites and at multiple times been conducted 10…”

Section: Introductionmentioning

confidence: 99%

<p>Comparative genomic analysis and multi-drug resistance differences of <em>Acinetobacter baumannii</em> in Chongqing, China</p>

Jian

Pan

et al. 2019

IDR

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IntroductionMultidrug-resistance in Acinetobacter baumannii has emerged as a serious problem to public health. There is still a significant gap in the understanding of the multidrug-resistance and the genome diversity evolutionary process of A. baumannii in China, especially in the central and western regions.MethodsTen A. baumannii strains were collected from three hospitals in Chongqing, China. Whole-genome re-sequencing was used to obtain differences in genomic levels among strains. The diversity were determined by multi-locus sequence typing method, and investigate the genetic relationship between the ten strains and others by phylogenetic analysis. Comparative analysis focused on resistance genes related to insertions and deletions (InDels) and single-nucleotide polymorphisms (SNPs) was performed.ResultsThe overall G+C% content was 39.05%~39.43%, the average sequencing depth was 273.95~428.99, and the alignment ratio of the sequencing data was 92.93%~99.27%. A total of 42 InDels and 11,387 SNPs were detected in the coding sequence region of the isolates. Phylogenetic tree shows that the 10 A. baumannii isolates were divided into four relative groups, and there exist the possibility of cross-regional spread pattern. A total number of 19 drug resistance genes had been found in each strain, and efflux pump-related genes accounted for the most. Only AacA4 underwent a change in InDel. Six types of drug resistance genes were found in the SNPs resistance gene-related loci, among which gene ANT(3’’)-II and QacE mutations were found in each strain.ConclusionIn this study, the main mechanism of A. baumannii multi-drug resistance is due to the multi-drug efflux pump related genes. The point mutations at the SNPs sites of the six types of resistance genes are the main differences in A. baumannii between Chongqing and the eastern coastal areas of China.

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Molecular Epidemiology of Multi-Drug Resistant Acinetobacter baumannii Isolated in Shandong, China

Cited by 21 publications

References 35 publications

Molecular Epidemiology of Metallo Beta Lactamases in Acinetobacter Baumannii at a Tertiary Care Hospital

Molecular Epidemiology of Metallo Beta Lactamases in Acinetobacter Baumannii at a Tertiary Care Hospital

Antimicrobial resistance of major clinical pathogens in South Korea, May 2016 to April 2017: first one-year report from Kor-GLASS

<p>Comparative genomic analysis and multi-drug resistance differences of <em>Acinetobacter baumannii</em> in Chongqing, China</p>

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