Molecular Epidemiology of Mycobacterium abscessus Isolates Recovered from German Cystic Fibrosis Patients

Wetzstein, Nils; Diricks, Margo; Kohl, Thomas A.; Wichelhaus, Thomas A.; Andres, Sönke; Paulowski, Laura; Schwarz, C.; Lewin, Astrid; Kehrmann, Jan; Kahl, Barbara C.; Dichtl, Karl; Hügel, Christian; Eickmeier, Olaf; Smaczny, Christina; Schmidt, Annika; Zimmermann, Stefan; Naehrlich, Lutz; Hafkemeyer, Sylvia; Niemann, Stefan; Maurer, Florian P.; Hogardt, Michael

doi:10.1128/spectrum.01714-22

Cited by 13 publications

(6 citation statements)

References 40 publications

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“…Some of these cases have appeared as outbreaks, sometimes related to contaminated environmental sources [ 95 , 96 , 97 , 98 ]. The introduction of molecular epidemiology tools has been of great importance for the characterization of these outbreaks because the phenotypic characteristics of this mycobacterium are so common that strain differentiation must be carried out by molecular analysis [ 11 , 97 , 99 , 100 ].…”

Section: Discussionmentioning

confidence: 99%

Updated Review on the Mechanisms of Pathogenicity in Mycobacterium abscessus, a Rapidly Growing Emerging Pathogen

2022

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In recent years, Mycobacterium abscessus has appeared as an emerging pathogen, with an increasing number of disease cases reported worldwide that mainly occur among patients with chronic lung diseases or impaired immune systems. The treatment of this pathogen represents a challenge due to the multi-drug-resistant nature of this species and its ability to evade most therapeutic approaches. However, although predisposing host factors for disease are well known, intrinsic pathogenicity mechanisms of this mycobacterium are still not elucidated. Like other mycobacteria, intracellular invasiveness and survival inside different cell lines are pathogenic factors related to the ability of M. abscessus to establish infection. Some of the molecular factors involved in this process are well-known and are present in the mycobacterial cell wall, such as trehalose-dimycolate and glycopeptidolipids. The ability to form biofilms is another pathogenic factor that is essential for the development of chronic disease and for promoting mycobacterial survival against the host immune system or different antibacterial treatments. This capability also seems to be related to glycopeptidolipids and other lipid molecules, and some studies have shown an intrinsic relationship between both pathogenic mechanisms. Antimicrobial resistance is also considered a mechanism of pathogenicity because it allows the mycobacterium to resist antimicrobial therapies and represents an advantage in polymicrobial biofilms. The recent description of hyperpathogenic strains with the potential interhuman transmission makes it necessary to increase our knowledge of pathogenic mechanisms of this species to design better therapeutic approaches to the management of these infections.

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Section: Discussionmentioning

confidence: 99%

Updated Review on the Mechanisms of Pathogenicity in Mycobacterium abscessus, a Rapidly Growing Emerging Pathogen

2022

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“…The adoption of WGS has markedly advanced our comprehension of NTM antibiotic resistance, thus improving treatment strategies. In a significant validation of WGS's precision, Wetzstein et al (166) demonstrated complete concordance between WGS forecasting of M. abscessus resistance to macrolides and aminoglycosides and results from both GenoType NTM-DR kits and phenotypic DST. Lipworth et al (167) leveraged sequencing data from M. abscessus to identify novel mutations in the erm gene and rrs gene linked to macrolide resistance, mutations not covered by traditional genotyping, raising the possibility of false-negative outcomes.…”

Section: Exploring Molecular Diagnostics For Ntm Infections: Beyond P...mentioning

confidence: 99%

Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification

Zhang,

Tang,

et al. 2024

Front. Public Health

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Non-tuberculous mycobacteria (NTM) infections pose a significant public health challenge worldwide, affecting individuals across a wide spectrum of immune statuses. Recent epidemiological studies indicate rising incidence rates in both immunocompromised and immunocompetent populations, underscoring the need for enhanced diagnostic and therapeutic approaches. NTM infections often present with symptoms similar to those of tuberculosis, yet with less specificity, increasing the risk of misdiagnosis and potentially adverse outcomes for patients. Consequently, rapid and accurate identification of the pathogen is crucial for precise diagnosis and treatment. Traditional detection methods, notably microbiological culture, are hampered by lengthy incubation periods and a limited capacity to differentiate closely related NTM subtypes, thereby delaying diagnosis and the initiation of targeted therapies. Emerging diagnostic technologies offer new possibilities for the swift detection and accurate identification of NTM infections, playing a critical role in early diagnosis and providing more accurate and comprehensive information. This review delineates the current molecular methodologies for NTM species and subspecies identification. We critically assess the limitations and challenges inherent in these technologies for diagnosing NTM and explore potential future directions for their advancement. It aims to provide valuable insights into advancing the application of molecular diagnostic techniques in NTM infection identification.

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“…43 Some studies have demonstrated clonally related strains in patients attending the same clinic 38,39,[44][45][46] and others have not. [47][48][49] DCC strains are not restricted to pwCF and have been frequently identified in patients with other respiratory diseases. 43,50 A national UK healthcare utilization database assessed potential contact between patients.…”

Section: Acquisition and Transmissionmentioning

confidence: 99%

Nontuberculous Mycobacteria in Cystic Fibrosis in the Era of Cystic Fibrosis Transmembrane Regulator Modulators

Burke

Thomson

Wainwright

et al. 2023

Semin Respir Crit Care Med

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Nontuberculous mycobacteria (NTM) are a group of mycobacteria which represent opportunistic pathogens that are of increasing concern in people with cystic fibrosis (pwCF). The acquisition has been traditionally though to be from environmental sources, though recent work has suggested clustered clonal infections do occur and transmission potential demonstrated among pwCF attending CF specialist centers. Guidelines for the screening, diagnosis, and identification of NTM and management of pwCF have been published. The emergence of CF-specific therapies, in particular cystic fibrosis transmembrane regulator (CFTR) modulator drugs, have led to significant improvement in the health and well-being of pwCF and may lead to challenges in sampling the lower respiratory tract including to screen for NTM. This review highlights the epidemiology, modes of acquisition, screening and diagnosis, therapeutic approaches in the context of improved clinical status for pwCF, and the clinical application of CFTR modulator therapies.

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Molecular Epidemiology of Mycobacterium abscessus Isolates Recovered from German Cystic Fibrosis Patients

Abstract: Mycobacterium abscessus is a major respiratory pathogen in cystic fibrosis (CF) patients. Recently it has been shown that dominant global clonal complexes (DCCs) have spread worldwide among CF patients.

Cited by 13 publications

References 40 publications

Updated Review on the Mechanisms of Pathogenicity in Mycobacterium abscessus, a Rapidly Growing Emerging Pathogen

Updated Review on the Mechanisms of Pathogenicity in Mycobacterium abscessus, a Rapidly Growing Emerging Pathogen

Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification

Nontuberculous Mycobacteria in Cystic Fibrosis in the Era of Cystic Fibrosis Transmembrane Regulator Modulators

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