Molecular Epidemiology of Rotaviruses in Nigeria: Detection of Unusual Strains with G2P[6] and G8P[1] Specificities

Adah, Mohammed I.; Wade, Abel; Taniguchi, Koki

doi:10.1128/jcm.39.11.3969-3975.2001

Cited by 93 publications

(120 citation statements)

References 35 publications

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“…Furthermore, we documented the first case of G1/G8 mixed infection in Iran. Also, the first reports of G1/G8 mixed infection have been documented in young children with gastroenteritis in Ireland and Nigeria [29,30]. We found mixed infections with two different rotavirus genotypes in 2.66 % of children, which is similar to previous studies, with a range of 1-26.4 % [31].…”

Section: Rotavirus Genotypingsupporting

confidence: 90%

Prevalence and Molecular Genotyping of Group A Rotaviruses in Iranian Children

Kargar

Akbarizadeh

2012

Indian J. Virol.

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Rotavirus is the leading cause of acute gastroenteritis in worldwide young children. Effective vaccines to prevent rotavirus infection are currently available, although their clinical use is still limited, and rotavirus still causes many episodes of infantile gastroenteritis, mainly during the winter season. The aim of this study was to evaluate the prevalence of rotavirus infection in children aged \5-years-old who were hospitalised for gastroenteritis. One hundred and sixty-three stool samples from hospitalised children (\5-years-old) complicated with severe diarrhoea, in two hospitals in Jahrom City, Iran were collected from 2009 to 2010. Antigenic prevalence of rotavirus group A was distinguished by enzyme immunoassay. The antigen of group A rotavirus was diagnosed by EIA in 75 of 163 collected samples. The genotype of EIA-positive samples was determined by nested RT-PCR. The frequency of rotavirus genotypes G1, G2, G3, G4 and G9 was 17.33, 13.34, 2.67, 30.66 and 2.67 %, respectively. Also, the frequency of mixed and non-typable genotypes was detected in 2.67 and 30.66 %, respectively. G1/G8 mixed infection was the first of these rotavirus genotypes to be reported in Iran. Detection of high prevalence of group A rotavirus infection in hospitalised children with diarrhoea, and determination of circulating rotavirus genotypes in this region of Iran, provide useful data for formulating effective vaccines; especially for infants less than 5-years-old.

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Section: Rotavirus Genotypingsupporting

confidence: 90%

Prevalence and Molecular Genotyping of Group A Rotaviruses in Iranian Children

Kargar

Akbarizadeh

2012

Indian J. Virol.

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“…The detection of G1 serotypes as the most common strain is not surprising as it is the most abundant rotavirus strain globally. 8,35,37,38 Rotavirus with VP4 P [6] genotype was the only genotype detected in this study. The P [6] genotype was previously thought to be associated with neonatal rotavirus strains and hence thought to be associated with asymptomatic infection.…”

Section: Discussionmentioning

confidence: 85%

Epidemiology of rotavirus and astrovirus infections in children in Northwestern Nigeria

et al. 2008

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Background: Recent estimates attribute 527 000 deaths in children less than five years of age to rotavirus diarrhea annually, with 145 000 occurring in sub-Saharan Africa. Human astroviruses have been identified as one of the most frequent causes of infantile diarrhea, second in incidence only to rotavirus. This study was conducted to determine the prevalence of rotavirus and astrovirus and also to establish the circulating strains of rotavirus in a community in Nigeria where most diarrheic patients do not visit clinics or health care centers. Methods: A total of 154 stool samples (134 diarrheic and 20 non-diarrheic) were collected from infants and young children less than 5 years of age from January-March 2002. Samples were obtained by house-to-house visit in randomly selected districts in Zaria, Northwestern Nigeria. The samples were screened for rotavirus and astrovirus antigens using commercially available Enzyme Linked Immunosorbent Assay (ELISA) kits. All positive group A rotavirus samples were further subjected to VP6 sub-group ELISA, Polyacrylamide gel electrophoresis (PAGE) to determine their RNA electropherotypes and Reverse transcription polymerase chain reaction (RT-PCR) to determine their VP7 and VP4 genotypes. Results: Rotavirus and astrovirus antigens were detected in 9% (12) and 5% (7) of the 134 diarrheic stool samples respectively. No viral antigen was detected in the non-diarrheic stools. Rotavirus infection was more common in younger children than astrovirus infection. VP6 sub-group II specificity (58.3%), long RNA electropherotypes (41.6%), VP7 genotype G1 (33.3%) and VP4 genotype P [6] (33.3%) were the most common strains in circulation at that time in the community. Of significance is the fact that a large proportion of the rotavirus strains in circulation could not be assigned either a VP6 subgroup or RNA electrophoretic pattern probably as a result of low viral load. Conclusion: In this community-based study, rotavirus and astrovirus were significantly associated with diarrhea. However, the prevalence of rotavirus infection among children appears to be low while that of astrovirus falls in the range seen in hospital-based studies around the continent. Rotavirus, astrovirus, diarrhoea, children, community, Nigeria Résume Contexte: Des estimations récentes attribuent 527 000 décès annuels d'enfants de moins de 5 ans à la diarrhée par rotavirus, parmi lesquels 145 000 surviennent en Afrique Sub Saharienne. Les astrovirus humains ont été identifiés comme l'une des causes les plus fréquentes de diarrhée infantile, en second lieu viennent les atrovirus. Cette étude a été menée pour déterminer la prévalence des rotavirus et astrovirus et établir les souches circulantes de rotavirus dans une population du Nigéria ou la plupart des patients diarrhéiques ne consultent pas en clinique ni les centres de santé. Key words: Epidemiology of Rotavirus and Astrovirus infection in Nigerian children. Aminu M. et al. Page | 169Méthode: Un total de 154 échantillons de selles (134 diarrhéiques et 20...

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“…1) associated with diarrhea in children in Burkina Faso. Adah et al [2001] and Salu et al [2003] reported about a decade ago the existence of this genotypes in the region. Recently, the importance of G2P [6] in Burkina Faso has been reported .…”

Section: Discussionmentioning

confidence: 99%

Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008–2010

Bonkoungou¹,

Damanka²,

Sanou³

et al. 2011

Journal of Medical Virology

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In this study, the diversity of G and P genotypes of rotavirus strains in Burkinabe children were examined. Between November 2008 and February 2010, 447 stool samples were collected from children <5 years of age with acute diarrhea visiting hospital in Ouagadougou. Group A rotavirus was previously detected in 151/447 (33.8%) of the samples tested by an immunochromatographic test and these samples were now tested further for rotavirus G and P genotypes by RT‐PCR. Of these, the rotavirus type genes were amplified by RT‐PCR for 140/151 (92.7%) samples and G and P genotypes were successfully determined for 81 (57.9%) and 130 (92.9%) samples, respectively. The most prevalent G genotypes were G1, 34/140 (24.3%), and G9, 21/140 (15%), while the predominant P genotypes were P[6], 56/140 (40%), and P[8], 54/140 (38.6%). Among the single infections, 63/140 (45%), the predominant G/P combinations were: G1P[8] (33%), G9P[8] (29%), and G2P[6] (14%). The unusual strains G1P[9] (3%), G12P[6] (3%), G10P[6] (2%), and G2P[8] (2%) were also detected. In a high number of strains 61/140 (43.6%), the G genotype could not be determined and mixed infections were determined in 17/140 (12.1%) of strains identified. This study highlights the high diversity and presence of unusual rotavirus strains in children in Burkina Faso. J. Med. Virol. 83:1485–1490, 2011. © 2011 Wiley‐Liss, Inc.

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Molecular Epidemiology of Rotaviruses in Nigeria: Detection of Unusual Strains with G2P[6] and G8P[1] Specificities

Cited by 93 publications

References 35 publications

Prevalence and Molecular Genotyping of Group A Rotaviruses in Iranian Children

Prevalence and Molecular Genotyping of Group A Rotaviruses in Iranian Children

Epidemiology of rotavirus and astrovirus infections in children in Northwestern Nigeria

Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008–2010

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