Molecular Epidemiology of the Community-Associated  Methicillin-Resistant Staphylococcus Aureus Clones: A Synthetic Review

Junie, Lia Monica; Jeican, Ionuț Isaia; Matros, L.; Pandrea, Stanca Lucia

doi:10.15386/cjmed-807

Cited by 20 publications

(20 citation statements)

References 33 publications

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“…ST80-IV is another predominant MRSA clone in Europe and was shown by phylogenetic analysis to have African origins [ 39 ]. This clone has been frequently reported in several countries of the Middle East including Egypt [ 37 , 38 , 39 ]. The ST80-IV MRSA isolate in our study was PVL-positive, a common feature of this clone [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital

Soliman

Elmanakhly

et al. 2020

Genes

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Methicillin-resistant Staphylococcus aureus (MRSA) strains are associated with serious complications and poor clinical outcome. In Egypt, they contribute to more than 70% of S. aureus healthcare-associated infections. This study combined whole-genome sequencing, bioinformatics, and statistical analyses to identify the phylogeny, resistome, virulome and potential genotype–phenotype–clinical correlation among 18 clinical isolates of MRSA in a tertiary hospital in Cairo, Egypt. The ST1535-V MRSA clone was the most frequently isolated (16.6%), followed by ST5-VI, ST1-V and ST239-III (11.1% each). SCCmec V, VI, IV and III types were detected at frequencies of 50%, 16.6%, 11.1% and 11.1%, respectively. None of the tested virulence genes were detected in all isolates, but they ranged in distribution from 1/18 to 17/18. The Panton–Valentine leukocidin (PVL)-encoding genes were detected in only four isolates and were enriched in isolates causing non-severe cases. Phylogenetic analysis revealed relatedness between three ST1535-Vs, two ST5-VIs, two ST239-IIIs and two ST1-Vs; however, only the two genetically related ST1-V isolates were epidemiologically linked. While disease outcome and source of infection had no correlation with a particular genotypic pattern, the sequence type was the most correlated factor with phylogeny and genotypic patterns, and a few genes were associated with non-severe cases.

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Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital

Soliman

Elmanakhly

et al. 2020

Genes

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“…In addition, the percentages of bacterial pathogen infection with MRSA patients have systemic disease (21.9%) was relatively higher than patients without systemic disease group (P = 0.001). MRSA is the most common bacterial pathogen infection that gives rise to a high burden of signi cant healthcare costs, morbidity, and mortality for patients every year [24][25][26]. The percentages of bacterial pathogen infection with P. aeruginosa tested positive made up 9.4% among patients with systemic disease group (P = 0.029).…”

Section: Discussionmentioning

confidence: 99%

Clinical features and etiology of patients with community-acquired pneumonia in southern China

Gan

Liu

Guo

et al. 2020

Preprint

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Background: The aim of this study was to investigate the clinical features and etiology in patients with community-acquired pneumonia (CAP) in southern China. Methods: A total of 342 patients who presented community-acquired pneumonia (CAP) from January 2019 to December 2019 were enrolled in this study. The respiratory pathogens in nine test and loop-mediated isothermal amplification (LAMP) detection were used to identify pathogens. Results: The mean age of this study population was 60.89 ± 18.87 years. The total incidences of CAP were more prevalent in males (60.5%, 207/342) than females (39.5%, 135/342), and the percentage was 65.8% (225/342) CAP patients in summer and autumn. The main causative pathogens were identified in 96/342 (28.1 %) patients. Of these, 14 (14.6 %) were MP infection, the most frequently isolated microorganism. Bacterial infection in the single infection was present in 47 (47/96, 49.0%). Mixed infections were demonstrated in 26 (26/96, 27.1%). MRSA infection was close to patients with systemic diseases (P = 0.001). Factors that were associated with systemic disease was the age >65 (OR 5.555, 95%CI 3.402-9.071, P<0.001). Conclusions: MP is common organisms isolated in community-acquired pneumonia. The year of above 65, the count of WBC and mixed pathogens infections may be associated with an increased risk of CAP patients with systemic disease.

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“…In addition, the proportions of bacterial pathogen infection with MecA (22.89%) was relatively high in aged >60 group ( P <.001) As is widely known, bacterial pathogen infection with MecA is inundant in Chinese hospitals and in many other parts of the world, being responsible for a high burden of significant healthcare costs, morbidity, and mortality every year. [29–31] The proportions of bacterial pathogen infection with Spn tested positive made up 5% among patients aged <15 group ( P <.001). Spn is an encapsulated diplococcus causing a wide spectrum of diseases ranging from such as pneumonia, meningitis, and sepsis to noninvasive diseases like otitis media and sinusitis and even death.…”

Section: Discussionmentioning

confidence: 99%

Clinical evaluation of the loop-mediated isothermal amplification assay for the detection of common lower respiratory pathogens in patients with respiratory symptoms

Hou

Zeng

et al. 2018

Medicine

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Lower respiratory tract infections (LRTIs) are a substantial public health problem and a leading cause of significant morbidity and mortality worldwide. The aim of this study was to evaluate a commercially available loop-mediated isothermal amplification (LAMP) assay for the simultaneously detection of thirteen common lower respiratory pathogens in patients with respiratory symptoms. All participants age from 1 to 101 years old were recruited from inpatient or outpatient of Meizhou People's Hospital between October 2016 and March 2018. A total of 1767 sputum samples and 88 bronchoalveolar lavage fluid samples from patients with suspected LRTI were collected. For each sample, a parallel study using both routine bacterial culture-based and LAMP assays were carried out. In total, 810 (44.85%) out of the 1855 samples were found to be positive infected with respiratory pathogens by using the LAMP assays. Methicillin-resistant Staphylococcus aureus (MecA) was the most predominant bacterial pathogens, with proportions of 17.09% in sputum and 10.23% bronchoalveolar lavage fluid samples, respectively. The proportions of bacterial pathogen infection with Streptococcus pneumoniae (Spn) (24.24%) was relatively high in aged <15 group (P <.001) while the proportions of bacterial pathogen infection with MecA (22.89%) was relatively high in aged >60 group (P <.001). Bacterial pathogen infection with MecA having the highest prevalence with proportions of 17.81% and 13.94% in male and female, respectively. A statistically higher proportion of male group had bacterial pathogen infection with Pseudomonas aeruginosa (Pae) in this study (P = .035). Comparison of results between the LAMP assay and culture method was conducted and our results indicated that there was higher detection rate by the LAMP assay than the bacterial culture method. Comparison of the results obtained with the LAMP assay and those obtained by sequencing analysis, when the sequencing method was set to 100%, demonstrating that the LAMP assay is 100% specific and 95.50% sensitive. The technique of LAMP assay was proved to be a simple, sensitive, specific, convenient, and rapid method, which can be implemented for diagnosing pathogenic bacteria in patients with LRTIs in primary labs without any need for expensive equipment or specialized techniques in resource-limited areas of China.

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Molecular Epidemiology of the Community-Associated Methicillin-Resistant Staphylococcus Aureus Clones: A Synthetic Review

Cited by 20 publications

References 33 publications

Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital

Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital

Clinical features and etiology of patients with community-acquired pneumonia in southern China

Clinical evaluation of the loop-mediated isothermal amplification assay for the detection of common lower respiratory pathogens in patients with respiratory symptoms

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