Molecular epidemiology of uropathogenic Escherichia coli

Yamamoto, Shingo

doi:10.1007/s10156-007-0506-y

Cited by 89 publications

(66 citation statements)

References 63 publications

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“…In most cases, uropathogenic clones are selected from the fecal flora, and the pathogenic potential of E. coli strains is thought to be dependent on the presence of VFs 19,23 . Based on their components and products called virulence factors, E. coli cells attach selectively to the mucosa uro-epithelium, promoting colonization and persisting in the urinary tract, inducing, then, a local inflammatory response and sometimes to promote tissue lesions 23,29 .…”

Section: Discussionmentioning

confidence: 99%

“…Virulence factors (VFs) associated with UPEC include adhesins (P fimbriae, type 1 fimbriae, S and F1C fimbriae, afimbrial adhesin), toxins (hemolysin, and cytotoxic necrotizing factor), siderophores (the aerobactin system) and polysaccharide coatings (group II capsules) 1,29 . Recently, a new urovirulence factor gene, which was more frequently found in UPEC strains, was reported.…”

Section: Introductionmentioning

confidence: 99%

“…PARRET & DE MOT (2002) 25 hypothesized that the usp protein may represent a novel type of E. coli bacteriocin, acting against competing E. coli strains that occupy the same niche, thereby enhancing their infectivity in the urinary tract environment. Recently, it was demonstrated that usp significantly enhanced the infectivity of E. coli in the mouse UTI model 29 .…”

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confidence: 99%

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Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Tiba

Yano

Leite

2008

Rev. Inst. Med. trop. S. Paulo

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Abbreviations: aerobactin (iucD), afimbrial adhesin (afaB/C), cytolethal distending toxin (cdtB), cytotoxic necrotizing factor type 1 (cnf-1), G adhesin classes of P fimbriae (papG alleles), group II capsule (kpsMTII), α-hemolysin (hly), minor structural subunits of P fimbriae (papE/F), outer membrane protein of P fimbrae (papC), polymerase chain reaction (PCR), S fimbriae (sfaC/D), type 1 fimbriae (fimH), urinary tract infection (UTI), uropathogenic specific protein (usp), uropathogenic Escherichia coli (UPEC), virulence factors (VFs SUMMARYAdhesins (P-fimbriae, S-fimbriae, type 1 fimbriae and afimbrial adhesin), toxins (α-hemolysin and cytotoxic necrotizing factor type 1), iron acquisition systems (aerobactin) and host defense avoidance mechanisms (capsule or lipopolysaccharide) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. In this work, 162 Uropathogenic Escherichia coli (UPEC) strains from patients with cystitis were genotypically characterized by polymerase chain reaction (PCR) assay. We developed three multiplex PCR assays for virulence-related genes papC, papE /F, papG alleles, fimH, sfa/foc, afaE, hly, usp, cdtB, iucD, and kpsMTII, all

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Tiba

Yano

Leite

2008

Rev. Inst. Med. trop. S. Paulo

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“…Non-pathogenic E. coli are widely distributed in nature and an important part of the normal flora in the human gastrointestinal tract. E. coli causing UTI are classified as uropathogenic E. coli (UPEC); these strains account for 75-90 % of uncomplicated UTIs but are also an important aetiological agent of bacteraemia, sepsis and meningitis (Russo & Johnson, 2000;Yamamoto, 2007).…”

Section: Introductionmentioning

confidence: 99%

Recurrent urinary tract infection due to co‐infection with extended spectrum β‐lactamase‐producer uropathogenic Escherichia coli and enteroaggregative E. coli

et al. 2014

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Introduction: Urinary tract infections (UTIs) are the most common bacterial infections in women of all ages. The emergence of resistance to newer classes of antibiotics, including third generation cephalosporins, has limited the drug choices for treatment of UTI. We report here a recurrent UTI due to co-infection with multiple-antibiotic-resistant (MAR) Escherichia coli pathotypes.Case presentation: A 73-year-old woman with diabetes mellitus type 2 presented with fever, nausea, vomiting, burning sensation, painful and frequent urination. She was diagnosed with recurrent UTI (RUTI) due to co-infection with pansensitive enteroaggregative E. coli (EAEC) and MAR extended spectrum b-lactamase (ESBL)-producing uropathogenic E. coli (UPEC) and treated with azithromycin and levofloxacin (each for 10 days). Unfortunately, she did not respond. Plasmid profile analysis showed that the MAR E. coli strain harboured multiple plasmids including ,38 and ,80 MDa plasmids. PCR for detection of b-lactamase genes showed that the isolate was negative for blaTEM, blaSHV, blaOXA and blaCTX-M. To the best of our knowledge, this is the first report of co-infection with an EAEC and MAR ESBL-producer UPEC in Bangladesh. Conclusion:We recommend that all pathotypes of E. coli as well as the other more common uropathogens should be considered in the diagnosis of RUTI and multiple antibiotics should be prescribed only in severe conditions.

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“…Uropathogenic virulence factors (VFs) have been identified by comparing the prevalence among urinary tract isolates with that of environmental strains. VFs include adhesins, iron uptake systems, cytotoxins, and specific O:H:K serotypes [1,2].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PAIusp subtyping to characterize uropathogenic E. coli isolates

Lai

Zaw

Shamsudin

et al. 2016

J Infect Dev Ctries

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Introduction: Uropathogenic virulence factors have been identified by comparing the prevalence of these among urinary tract isolates and environmental strains. The uropathogenic-specific protein (USP) gene is present on the pathogenicity island (PAI) of uropathogenic Escherichia coli (UPEC) and, depending on its two diverse gene types and the sequential patterns of three open reading frame units (orfUs) following it, there is a method to characterize UPEC epidemiologically called PAIusp subtyping. Methodology: A total of 162 UPEC isolates from Sabah, Malaysia, were tested for the presence of the usp gene and the sequential patterns of three orfUs following it using polymerase chain reaction (PCR). In addition, by means of triplex PCR, the prevalence of the usp gene was compared with other two VFs of UPEC, namely alpha hemolysin (α-hly) and cytotoxic necrotizing factor (cnf-1) genes encoding two toxins. Results: The results showed that the usp gene was found in 78.40% of UPEC isolates, indicating that its prevalence was comparable to that found in a previous study in Japan. The two or three orfUs were also associated with the usp gene in this study. All the PAIusp subtypes observed in Japan were present in this study, while subtype IIa was the most common in both studies. The usp gene was observed in a higher percentage of isolates when compared with ɑ-hly and cnf-1 genes. Conclusions: The findings in Japan and Sabah, East Malaysia, were similar, indicating that PAIusp subtyping is applicable to the characterization of UPEC strains epidemiologically elsewhere in the world.

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Molecular epidemiology of uropathogenic Escherichia coli

Cited by 89 publications

References 63 publications

Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

Recurrent urinary tract infection due to co‐infection with extended spectrum β‐lactamase‐producer uropathogenic Escherichia coli and enteroaggregative E. coli

Evaluation of PAIusp subtyping to characterize uropathogenic E. coli isolates

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