Molecular Epidemiology, Virulence Traits and Antimicrobial Resistance Signatures of Aeromonas spp. in the Critically Endangered Iberochondrostoma lusitanicum Follow Geographical and Seasonal Patterns

Grilo, Miguel L.; Isidoro, Sara; Chambel, Lélia; Marques, Cristina; Marques, Tiago A.; Sousa‐Santos, Carla; Robalo, Joana Isabel; Oliveira, Manuela

doi:10.3390/antibiotics10070759

Cited by 6 publications

(2 citation statements)

References 127 publications

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“…In this study, four different Aeromonas species (A. hydrophila, A. caviae, A. veronii sobria, and A. veronii veronii) were isolated with A. hydrophila being the most dominant species this is consistent with the findings of Perretta et al (2018) and Borella et al (2020). However, it is different from the results of Ashiru et al (2017) and Grilo et al (2021) who opined differences in the most prevalent of the phenospecies of Aeromonas from the fish farm. This finding is most likely as a result of the diverse Aeromonas species present and their ability to adapt to the aquatic environment successfully leading to their widespread distribution.…”

Section: Prevalence Of the Different Species Of Aeromonas From Fish F...supporting

confidence: 77%

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

2023

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Aeromonas species are associated with diseases on fish farms leading to mortality and economic losses. In order to determine the prevalence and diversity of Aeromonas species from water and Clarias gariepinus cultured in the different holding facilities; five hundred and seventy six Clarias gariepinus fish and one hundred and eight water samples were randomly sampled from fish farms in Kwara State. The samples were clinically and bacteriologically examined and confirmed using Oxiod rapid microbat identification test kits for Gram-negative bacteria, Microbact 24E (MB24E). The overall prevalence of Aeromonas species from water from the different cultured facilities revealed water sampled from earthen ponds had the highest prevalence of 66.67 % followed by concrete tanks with a prevalence of 41.66 % and the least prevalence of 30.56 % was recorded for water in plastic tanks. A similar pattern was recorded in Clarias gariepinus from the different holding facilities showing that earthen ponds had the highest prevalence of 40.10 % when compared with concrete tanks 28.65%, and to plastic tanks 20.83 %. Four varying diversities of Aeromonas species comprising of Aeromonas hydrophila, Aeromonas caviae, Aeromonas veronii biovar sobria, and Aeromonas veronii biovar veronii were isolated from both water and fish from the different holding facilities. From this study, cultured Clarias gariepinus were susceptible to Aeromonas species irrespective of the culture facilities. Therefore, there is need for proper management practices and adherence to biosecurity measures to prevent the outbreak and spread of diseases on the farms.

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Section: Prevalence Of the Different Species Of Aeromonas From Fish F...supporting

confidence: 77%

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

2023

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“…Aeromonas species selection followed results obtained prior to this study [ 52 ]. Namely, the occurrence of mesophilic Aeromonas spp.…”

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confidence: 99%

Climatic Alterations Influence Bacterial Growth, Biofilm Production and Antimicrobial Resistance Profiles in Aeromonas spp.

et al. 2021

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Climate change is expected to create environmental disruptions that will impact a wide array of biota. Projections for freshwater ecosystems include severe alterations with gradients across geographical areas. Life traits in bacteria are modulated by environmental parameters, but there is still uncertainty regarding bacterial responses to changes caused by climatic alterations. In this study, we used a river water microcosm model to evaluate how Aeromonas spp., an important pathogenic and zoonotic genus ubiquitary in aquatic ecosystems, responds to environmental variations of temperature and pH as expected by future projections. Namely, we evaluated bacterial growth, biofilm production and antimicrobial resistance profiles of Aeromonas species in pure and mixed cultures. Biofilm production was significantly influenced by temperature and culture, while temperature and pH affected bacterial growth. Reversion of antimicrobial susceptibility status occurred in the majority of strains and tested antimicrobial compounds, with several combinations of temperature and pH contributing to this effect. Current results highlight the consequences that bacterial genus such as Aeromonas will experience with climatic alterations, specifically how their proliferation and virulence and phenotypic resistance expression will be modulated. Such information is fundamental to predict and prevent future outbreaks and deleterious effects that these bacterial species might have in human and animal populations.

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Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence

et al. 2022

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Aeromonas veronii is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (ΔascP) by homologous recombination. The results showed that the deletion of the ascP gene significantly down-regulated the expression of associated effector proteins in A. veronii compared to its wild type. The adhesive and invasive abilities of ΔascP to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of ΔascP to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD 50 of the mutant strain of crucian carp was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the ΔascP content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in crucian carp after the infection of the ΔascP strain were considerably lower in contrast to the wild strain. Hence, the ascP gene is essential for the etiopathogenesis of A. veronii TH0426.

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Molecular Epidemiology, Virulence Traits and Antimicrobial Resistance Signatures of Aeromonas spp. in the Critically Endangered Iberochondrostoma lusitanicum Follow Geographical and Seasonal Patterns

Cited by 6 publications

References 127 publications

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

Climatic Alterations Influence Bacterial Growth, Biofilm Production and Antimicrobial Resistance Profiles in Aeromonas spp.

Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence

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