Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma

Smieško, Gordana; Banović, Pavle; Gusman, Vera; Simin, Verica; Cîmpean, Anca Maria; Lalošević, Dušan

doi:10.1007/s10735-020-09886-5

Cited by 2 publications

(3 citation statements)

References 38 publications

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“…Most reports of fibrosarcomas in rodents come from laboratory animal experiments (Gilson et al 1990, Prakash et al 2001, Smieško et al 2020. Spontaneous fibrosarcomas in limbs have been reported in pet degus (Octodon degus) (Svara et al 2020), similar to Case 2.…”

Section: Discussionmentioning

confidence: 71%

Clinical, pathological and immunohistochemical characterization of spontaneous neoplasms in pet rodents in Northeastern Brazil

Pereira,

Lima,

Oliveira

et al. 2024

Pesq. Vet. Bras.

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In the last decade, there has been a significant increase in the demand for small rodents such as hamsters, guinea pigs, rats, and mice to be kept as pets. Consequently, the veterinary care provided to these animals has also increased. The aim of this study was to describe gross, histopathological and immunohistochemical findings of 26 spontaneous neoplasms diagnosed in 25 pet rodents in Northeastern Brazil. A retrospective study was carried out from 2014 to 2022 in two veterinary diagnostic laboratories to identify cases of tumoriform lesions in these species. Hamsters represented the most frequent species in this study (60%, 15/25), followed by rats (28%, 7/25), guinea pigs (8%, 2/25), and mice (4%, 1/25), with a mean age of 17.63 months. The anatomical regions of the face/head and thoracic region were the most affected. The most affected tissues were the skin/subcutaneous/mucosal (65%, 15/26) and mammary gland (23%, 6/26). Eighteen different types of neoplasms were diagnosed, and T-cell lymphomas and mammary adenocarcinomas were the most diagnosed tumors, each corresponding to 12% (3/26) of the cases. Leiomyosarcoma, myxosarcoma and mammary adenocarcinoma metastases were also noted. Immunohistochemistry was essential for the diagnosis of certain malignant mesenchymal and round-cell neoplasms. Pet rodent neoplasms in Northeastern Brazil are common, especially in hamsters, and immunohistochemistry can be a useful tool for the definitive diagnosis of these tumors.

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Section: Discussionmentioning

confidence: 71%

Clinical, pathological and immunohistochemical characterization of spontaneous neoplasms in pet rodents in Northeastern Brazil

Pereira,

Lima,

Oliveira

et al. 2024

Pesq. Vet. Bras.

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“…Neka istraživanja su, proučavajući uticaj stresa na ženkama, nailazila na poteškoće pronalaska validnog modela za socijalni hronični stres (11). Model imobilizacionog stresa se smatra široko primenjivanim, te se koristi se za ispitivanje različitih sistema u stresogenim okolnostima, poput endokrinog, imunog i nervnog (12,13,14). Uticaj stresa na ženke pacova i miševa može se pratiti analizom estrusnog ciklusa, koji je pod direktnim uticajem hormona hipotalamohipofizno gonadne osovine.…”

Section: Uvodunclassified

“…Imobilizacioni stres je izazvan ubacivanjem životinja u imobilizacione komore 3 cm širine i 11 cm dužine i onemogućeno im je pomeranje repa, ekstremiteta, tela i glave. Frontalni deo komore je difuzno perforiran kako bi se omogućio protok vazduha za normalno disanje životinja (13,14).…”

Section: Eksperimentalni Dizajnunclassified

The impact of chronic restraint stress on the estrous cycle in NMRI female mice

Ristić¹,

Bogdan²,

Banović³

2021

Medicinski podmladak

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Introduction: Stress represents a set of reactions in the organism activated by external factors. In order to maintain homeostasis and protect the organism, numerous mechanisms for adaptation to stress evolved. Stressors that act in short-term period cause acute stress reaction with generally positive effect on organism. When the stressor persists, and the organism fails to respond to the challenge, chronic stress develops, leading to pathological conditions, such as women's menstrual cycle disorders. Aim: To examine the impact of chronic restraint stress on the estrous cycle in NMRI female mice. Material and methods: A number of 12 mature female NMRI mice were randomly divided into control (n = 6) and experimental (n = 6) group. The induction of stress was performed for the experimental group by using restrain chambers 2 hours daily in 14 days. From the beginning of the experiment, vaginal lavages were taken from all mice for making smears that were analyzed to determine estrous cycle stages. For analysis of chronic stress effect, the frequency of estrus stages alternation observed in experimental group compared to control was examined. Results: The estrous cycle was observed and divided into proestrus, estrus, metestrus and diestrus. During the adaptation period, prolonged diestrus was dominantly present in both groups. When stress was induced, in the experimental group animals the absence of diestrus stage and oscillation to other stages was obseved, in contrast to the control group, where the diestus stage was frequently observed. Frequency of diestrus stage deviation in stressed animals was shown to be statistically significant (p < 0.01) when compared to control. Conclusion: The induction of chronic restraint stress in female NMRI mice leads to the alternation of the estrous cycle. Considering the fact that NMRI female mice share the similar hormonal regulation of the estrous cycle with women's menstrual cycle, they could present a solid model for studying women's reproductive disorders.

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Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma

Cited by 2 publications

References 38 publications

Clinical, pathological and immunohistochemical characterization of spontaneous neoplasms in pet rodents in Northeastern Brazil

Clinical, pathological and immunohistochemical characterization of spontaneous neoplasms in pet rodents in Northeastern Brazil

The impact of chronic restraint stress on the estrous cycle in NMRI female mice

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