Molecular evidence for the existence of additional members of the order Chlamydiales

Ossewaarde, Jacobus M.; Meijer, Adam

doi:10.1099/13500872-145-2-411

Cited by 112 publications

(123 citation statements)

References 33 publications

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“…Previous reports on new chlamydial organisms were from clinical samples (2,4,8,12,14,15), and environmental isolates concerned exclusively new phylotypes of parachlamydiae (9)(10)(11). Our data and those of Bowman et al (3) indicated that new distinct chlamydial lineages exist in natural environments as different as Antarctic marine salinity lake sediments and European freshwater ponds.…”

Section: New Chlamydial Lineages From Freshwater Samplessupporting

confidence: 71%

“…Simkania negevensis is another emerging chlamydia, isolated initially as a cell culture contaminant and successively associated with human respiratory infection in Israel, Great Britain and North America (8,12). The diversity within chlamydiae is likely to be more important : using PCR, new 16S rDNA phylotypes have been detected in clinical samples (4,14), and sequence databases contain over 100 partial 16S rDNA sequences apparently belonging to distinct chlamydial species. Increasing diversity within Chlamydiales is also shown by results reported by Bowman et al (3), who found 16S rDNA sequences from sediments of Antarctic marine salinity lakes (Burton-46 ; 999 bp) and a basin (Taynaya-24 ; 995 bp).…”

Section: New Chlamydial Lineages From Freshwater Samplesmentioning

confidence: 99%

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Root colonization by Pseudomonas putida: love at first sight

2002

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Section: New Chlamydial Lineages From Freshwater Samplessupporting

confidence: 71%

Section: New Chlamydial Lineages From Freshwater Samplesmentioning

confidence: 99%

Root colonization by Pseudomonas putida: love at first sight

2002

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“…The chlamydial group of species until very recently consisted of a single family, the Chlamydiaceae, which contained one genus and four species then named Chlamydia trachomatis, Chlamydia pecorum, Chlamydia pneumoniae and Chlamydia psittaci (Fields & Barnes, 1992 ;Bush & Everett, 2001 ;Meijer et al, 1999 ;Pettersson et al, 1997 ;Fukushi & Hirai, 1992 ;Grayston et al, 1989 ;Moulder et al, 1984 ;Page, 1968). However, the taxonomy of this group has undergone a major revision within the last three years.…”

Section: Discussionmentioning

confidence: 99%

“…caviae, Chlam. pneumoniae and Chlamydophila pecorum (Bush & Everett, 2001 ;Herrmann et al, 2000). In addition to Chlamydiaceae, the proposal recognizes two new families of chlamydialike organisms, Parachlamydiaceae and Simkaniaceae Protein signatures for chlamydiae (Bush & Everett, 2001 ;Everett et al, 1999 ;Kahane et al, 1999 ;Ossewaarde & Meijer, 1999). A fourth family of chlamydia-like organisms, Waddliaceae, was recognized in a separate proposal (Rurangirwa et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the traditional chlamydial species, which are placed in the family Chlamydiaceae, a number of other organisms that show chlamydia-like replication cycles have been identified (Everett et al, 1999 ;Kahane et al, 1999 ;Ossewaarde & Meijer, 1999 ;Rurangirwa et al, 1999). Based on the 16S and 23S rRNA phylogenies, these organisms have been placed in three separate families : Parachlamydiaceae, Waddliaceae and Simkaniaceae, within the order Chlamydiales (Everett et al, 1999 ;Rurangirwa et al, 1999).…”

Section: Presence Of the Identified Signatures In Chlamydialike Organmentioning

confidence: 99%

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Protein signatures distinctive of chlamydial species: horizontal transfers of cell wall biosynthesis genes glmU from archaea to chlamydiae and murA between chlamydiae and Streptomyces a aThe GenBank accession numbers for the sequences reported in this paper are indicated in the text.

Griffiths

Gupta

2002

Microbiology

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Chlamydiae are major human and animal pathogens. Based on alignments of different protein sequences, a number of conserved indels (insertion/deletions) were identified that appear to be unique and distinctive characteristics of the chlamydial species. The identified signatures include one 16 aa and two single aa inserts in the enzyme UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA), a 1 aa insert in protein synthesis elongation factor P (EF-P), a 1 aa insert in the Mg 2M transport protein (MgtE), a 1 aa insert in the carboxyterminal protease and a 1 aa deletion in the tRNA (guanine-N 1 -)-methyltransferase (TrmD) protein. The homologues of these proteins are found in all major groups of bacteria and the observed indels are present in all available chlamydial sequences but not in any other species (except for the large insert in MurA in Streptomyces). The validity of three of these signatures (MurA, EF-P and MgtE) was tested by PCR amplifying the signature regions from several chlamydial species for which no sequence information was available. All Chlamydiaceae species for which specific fragments could be amplified (Chlamydia suis, Chlamydophila abortus, Chlamydophila psittaci, Chlamydophila felis) contained the expected signatures. Additionally, a fragment of the murA gene from Waddlia chondrophila and the efp gene from Simkania negevensis, two chlamydia-like species, were also cloned and sequenced. The presence of respective indels in these species provides strong evidence that they are specifically related to the traditional chlamydial species, and that these signatures may be distinctive of the entire Chlamydiales order. A 17 aa conserved indel was also identified in the cell wall biosynthesis enzyme UDP-N-acetylglucosamine pyrophosphorylase (GlmU), which is shared by all archaeal and chlamydial homologues. The gene for this protein is indicated to have been horizontally transferred from an archaeon to a common ancestor of the chlamydiae. The results also support a lateral transfer of the murA gene between chlamydiae and Streptomyces. The large inserts in these peptidoglycan synthesis related genes in chlamydiae could account for their unusual cell-wall characteristics. These signatures are also potentially useful for screening of the chlamydiae species.

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Chlamydiae

Read¹

2005

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

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The chlamydiae are a group of obligate intracellular pathogens with a unique biphasic life cycle. The six genomes sequenced are approximately 1.05–1.2 Mb and show no signs of pathogenicity islands or other recent horizontal transfer events. There are about 750 genes conserved across all genomes, encoding core functions. Potential virulence determinants include type III secretion systems, toxins, variable surface proteins, and tryptophan biosynthesis proteins.

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Molecular evidence for the existence of additional members of the order Chlamydiales

Cited by 112 publications

References 33 publications

Root colonization by Pseudomonas putida: love at first sight

Root colonization by Pseudomonas putida: love at first sight

Protein signatures distinctive of chlamydial species: horizontal transfers of cell wall biosynthesis genes glmU from archaea to chlamydiae and murA between chlamydiae and Streptomyces a aThe GenBank accession numbers for the sequences reported in this paper are indicated in the text.

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