Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent

Abstract

The most frequently used method for establishing epidemiological relationships between Plesiomonas shigelloides strains is O:H serotyping. However, a number of strains are not serotypeable and isolates from diverse sources can display the same serovar. Moreover, since the zoonotic nature of Plesiomonas has been suggested and this hypothesis is based on the identical serovars found in animals and humans, we intend to use four DNA-based techniques: random amplified polymorphic DNA-PCR, enterobacterial repetitive intergenic consensus-PCR, repetitive extragenic palindromic-PCR, and pulsed field gel electrophoresis in order to screen 24 strains belonging to nine O:H serovars isolated from humans, animals, and the environment. In general, P. shigelloides showed a high genetic heterogeneity. Three pairs of strains, each containing a human and an animal isolate, displayed similar genotypes. This is the first report that provides molecular evidence that P. shigelloides may be zoonotic.

Plesiomonas shigelloides Revisited

“…Plesiomonas has also been recovered from 3.9% to 7.4% of ring-billed and herring gull feces on bathing beaches located on southwestern Lake Michigan (71). Other avian species reported to yield P. shigelloides include whooper swan, black stork, goldeneyes, herons, and penguins (72,73,74).…”

“…Detailed investigations of their relative frequencies in such hosts have not been published. These taxa include reptiles (alligator, lizard), cats, dogs, hares, red wolf, foxes, and a roach (42,72,74,75,76). Table 2 lists a compilation of animal species from which Plesiomonas has been recovered since 2000.…”

Effects of partially replacing dietary soybean meal or cottonseed meal with completely hydrolyzed feather meal (defatted rice bran as the carrier) on production, cytokines, adhesive gut bacteria, and disease resistance in hybrid tilapia (Oreochromis niloticus ♀ × Oreochromis aureus ♂)

“…and A. aquariorum [35,36] in the present study, which were decreased in the CHFM12/CSM group, are potentially harmful species in aquaculture. The recently described A. aquariorum is associated with a variety of human diseases [37], and Plesiomonas shigelloides, which is regarded as an emerging enteric pathogen, is also a possible zoonotic agent [38]. Our study showed that CHFM induced adaptation of the intestinal microbiota in tilapia and possibly prevented the intestinal tract from becoming colonized by potentially harmful species.…”

Isolation, Identification and Genomic Analysis of Plesiomonas shigelloides Isolated from Diseased Percocypris pingi (Tchang, 1930)

“…Plesiomonas shigelloides, an emerging pathogen of humans and animals, is widespread in the aquatic environment. They have been isolated from various different animals, such as shellfish, freshwater fish, snakes, dogs, goats, cats, swine and monkeys (González-Rey et al, 2011). Interestingly, not all farmed fishes were infected with P. shigelloides, however, this bacterium is a common source of disease in grass carp, tilapia, Chinese giant salamander (Andrias davidianus) and black carp in China.…”

“…Thus far, P. shigelloides has been isolated from streams, lakes, estuarine waters, mammals, crustaceans, mollusks, reptiles, amphibians and fish (Alexander et al, 2016). P. shigelloides is an emerging enteric pathogen that is linked to intestinal and extra-intestinal infections in humans (González-Rey et al, 2011). It is recognized as a potential fish pathogen and has been isolated from red hybrid tilapia (Nadirah et al, 2012).…”