Molecular evidence reveals presence of Rhizoctonia solani AG 1-IB on Tagetes patula in India

Abstract

Rhizoctonia solani was isolated from marigold (Tagetes patula) showing web blight symptoms in Meghalaya, India. Fluorescence microscopy was used to confirm its multinucleate character. Molecular characterization using ITS1-5.8-ITS2 region of nrDNA proved that it belonged to AG1-IB subgroup. To our knowledge, this is the first record of AG1-IB on marigold from India. Keywords Rhizoctonia solani. Tagetes patula, AG1-IB French marigold (Tagetes patula L.) is an important flower crop grown on commercial scale in India. Leaf and flower extracts have medicinal properties. Carotenoids are used in cancer therapy (Naik et al. 2003). Dye is used in textile industry and colours in food industry. Essential oil has been reported to possess anticandidial activity (Dutta et al. 2007). Marigold flowers of Indian variety contain lutein ester which is a natural antioxidant (Bhattacharyya et al. 2010). Reports regarding the use of T. patula in phytoremediation have also been published (Sun et al. 2011).

Pathogenicity of Rhizoctonia solani AG 1-IB on common weeds in Meghalaya

“…Phylogenetic analysis using maximum parsimony also clustered the isolate with AG 1-IB with 81% bootstrap support (Figure 1). Baiswar et al (2012) have also identified AG 1-IB on soybean and marigold using the primers developed by Matsumoto (2002) and Saylor and Yang (2007). Phylogenetic approach utilizing ITS region has also been used by several workers for accurate identification (Baiswar 2012;Mahendra et al 2016 andSavenai et al 2017).…”

Pathogenicity of <I>Rhizoctonia solani</I> AG 1-IA on Major Weeds Prevalent in Rice and Maize Ecosystem in Meghalaya

“…Matsumoto (2002) and Saylor and Yang (2007), have designed specifically primed PCR for direct detection of AG 1 and AG 2 subgroups and using these primers, the four isolates SRS, FRS, RSM1 and RSM2 were identified as AG 1 IA. Baiswar et al (2012 and have also identified AG 1-IA on rice and maize, and AG 1 IB on soybean, marigold using the primers developed by Matsumoto (2002) and Saylor and Yang (2007).…”

A rapid colorimetric LAMP assay for detection of Rhizoctonia solani AG-1 IA causing sheath blight of rice

“…Globally, rice production suffers from various diseases and rice sheath blight is one of the major disease among them. Rhizoctonia solani is a ubiquitous pathogen of rice, vegetables 2 , grains 3 , grasses 4 , etc. causing sheath blight which is the second most devastating disease in rice 5 , 6 .…”