Molecular evidence supports hypervariability in Phytophthora colocasiae associated with leaf blight of taro

Nath, Vishnu Sukumari; Senthil, Muthukrishnan; Hegde, Vinayaka; Jeeva, M. L.; Misra, R. S.; Veena, S; Raj, Mithun

doi:10.1007/s10658-013-0181-z

Cited by 7 publications

(10 citation statements)

References 34 publications

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“…Plant material and P. colocasiae strain P. colocasiae strain PC2 was isolated from leaf blight infected samples and maintained on Carrot Agar (CA; 250 g l À1 carrot and 20 g l À1 agar) slants at 15 C in the dark [10]. Virulence was maintained by infecting detached taro leaves and re-isolation every 2e3 months.…”

Section: Methodsmentioning

confidence: 99%

Identification of Phytophthora colocasiae genes differentially expressed during infection on taro (Colocasia esculenta)

Nath

Rajitha

Darveekaran

et al. 2015

Physiological and Molecular Plant Pathology

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Section: Methodsmentioning

confidence: 99%

Identification of Phytophthora colocasiae genes differentially expressed during infection on taro (Colocasia esculenta)

Nath

Rajitha

Darveekaran

et al. 2015

Physiological and Molecular Plant Pathology

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“…For artificial inoculation of leaves, a rapid method described by Nath et al . () was used. Artificial infection of taro tubers was carried out according to Mishra et al .…”

Section: Methodsmentioning

confidence: 99%

“…Isolates of P. colocasiae used in this study were isolated from taro blight samples collected from various geographical boundaries of India during a period of 2007-2012. Isolation and maintenance of the P. colocasiae isolates were performed as previously described (Nath et al, 2013). The isolates were confirmed to species level by ITS amplification and sequencing using universal ITS1 and ITS4 primers (White et al, 1990).…”

Section: Biological Materialsmentioning

confidence: 99%

“…It has been reported that the number of rDNA copies fluctuates with age and stage of growth of the organism and this inconsistency may affect the accurate quantification of pathogen and potential comparison between samples (Attallah et al, 2007). Moreover, our recent study has shown that ITS region is highly variable in P. colocasiae with considerable amount of genetic diversity existing among isolates obtained from fine spatial scale (Nath et al, 2013). These findings suggest that ITS region may not be a suitable target for specific detection of P. colocasiae.…”

Section: Introductionmentioning

confidence: 99%

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Rapid and sensitive detection ofPhytophthora colocasiaeresponsible for the taro leaf blight using conventional and real-time PCR assay

Nath

Hegde

Jeeva

et al. 2014

FEMS Microbiol Lett

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Conventional and real-time PCR assays were developed for sensitive and specific detection of Phytophthora colocasiae, an oomycete pathogen that causes leaf blight and corm rot of taro. A set of three primer pairs was designed from regions of the RAS-related protein (Ypt1), G protein alpha-subunit (GPA1) and phospho-ribosylanthranilate isomerase (TRP1) genes. In conventional PCR, the lower limit of detection was 50 pg DNA, whereas in real-time PCR, the detection limit was 12.5 fg for the primer based on Ypt1 gene. The cycle threshold values were linearly correlated with the concentration of the target DNA (range of R(2) = 0.911-0.999). All the primer sets were successful in detecting P. colocasie from naturally infected leaves and tubers of taro. Phytophthora colocasiae was detected from artificially infested samples after 18 and 15 h of postinoculation in conventional and real-time PCR assay, respectively. The developed PCR assay proved to be a robust and reliable technique to detect P. colocasiae in taro planting material and for assessing the distribution of pathogen within fields, thus aid in mitigating taro leaf blight.

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“…Taro (Colocasia esculenta), a member of the Araceae family is an ancient crop widely grown throughout the humid tropics for its edible leaves, petioles and corms (Nath et al, 2013). It is ranked fifth behind potato, cassava, sweet potato and yam in terms of global production (Akwee et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Dry matter accumulation and partitioning of two taro (Colocasia Esculenta (L.) Schott) cultivars under Inceptisol soils in Samoa

Faamatuainu¹,

Amosa²

2016

S. Pac. J. Nat. App. Sci.

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Taro (Colocasia esculenta) crop is widely grown throughout the humid tropics for its edible leaves, petioles and corms. In this research total dry matter (DM) accumulation and its partitioning between different plant parts were studied to determine their growth pattern over the plants ' life cycle (i.e. 35, 70, 105, 140 and 175 days after planting (DAP). An experiment with randomised complete block design (RCBD) having five treatments (DAP) and three replications (blocks) was setup for each of the two improved taro cultivars (Samoa 1 and Samoa 2). After harvest dry matter of each taro plant was estimated for its five parts: leaf blades, petioles, corms, roots and suckers. The data analysis showed that in both the cultivars the DM accumulation and its partitioning to different plant parts (i.e. the leaf blades (LDM), petioles (PDM), roots (RDM), corms (CDM) and suckers (SDM)) varied significantly (p < 0.001) over the five growth stages of plant growth (DAP).

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Molecular evidence supports hypervariability in Phytophthora colocasiae associated with leaf blight of taro

Cited by 7 publications

References 34 publications

Identification of Phytophthora colocasiae genes differentially expressed during infection on taro (Colocasia esculenta)

Identification of Phytophthora colocasiae genes differentially expressed during infection on taro (Colocasia esculenta)

Rapid and sensitive detection ofPhytophthora colocasiaeresponsible for the taro leaf blight using conventional and real-time PCR assay

Dry matter accumulation and partitioning of two taro (Colocasia Esculenta (L.) Schott) cultivars under Inceptisol soils in Samoa

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