Molecular Evolution of the Internal Transcribed Spacers (ITS1 and ITS2) and Phylogenetic Relationships among Species of the Family Cucurbitaceae

Jobst, Jürgen; King, Klaus; Hemleben, Vera

doi:10.1006/mpev.1997.0465

Cited by 119 publications

(78 citation statements)

References 63 publications

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“…Seed morphology provides a number of characteristics that are potentially useful for species identification, phylogenetic inference, and character-state evolution (Jobst et al 1998;Johnson et al 2004;Attar et al 2007;Moazzeni et al 2007;Mostafavi et al 2013). Observations of many plant groups have shown that seed morphology and anatomic features are rather conservative, and hence taxonomically important (Barthlott 1981;1984;Werker 1997;Abdel Khalik 2010;Abdel Khalik & Hassan 2012;Hassan & Abdel Khalik 2014).…”

Section: Introductionmentioning

confidence: 99%

Seed coat diversity in some tribes of Cucurbitaceae: implications for taxonomy and species identification

Heneidak

Khalik

2015

Acta Bot. Bras.

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Abstract:To evaluate their diagnostic value in systematic studies, seed coat morphology for 16 taxa from 11 genera of Cucurbitaceae were examined using stereomicroscopy and scanning electron microscopy. The taxa included representatives of the tribes Benincaseae, Bryonieae, Coniandreae, and Luffeae in order to evaluate their diagnostic value in systematic studies. Macro-and micromorphological characters of their seeds are presented, including shape, color, size, surface, epidermal cell shape, anticlinal boundaries, and periclinal cell wall. The taxonomic and phylogenetic implications of seed coat micromorphology were compared with those of the available gross morphological and molecular data. Seed character analysis offered useful data for evaluating the taxonomy of Cucurbitaceae on both intrageneric and tribal levels. Monophyly of the tribes Bryonieae, Coniandreae, and Luffeae was supported. Moreover, these analyses supported previous biochemical and phylogenetic data, indicating that distinct lineages are present within the tribe Benincaseae, that this tribe is not monophyletic, and that the subtribe Benincasinae is highly polyphyletic. A key is provided for identifying the investigated taxa based on seed characters.

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Section: Introductionmentioning

confidence: 99%

Seed coat diversity in some tribes of Cucurbitaceae: implications for taxonomy and species identification

Heneidak

Khalik

2015

Acta Bot. Bras.

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“…Knowledge regarding plant trait variability and the association of specific traits with hybridization not only facilitates breeding programs but also helps to design future germplasm collections. Molecular data have played a vital role in understanding the genetic relationships among various plants and have led to new genetic classifications that often conflict with traditional taxonomy (Jobst et al, 1998). Genotypes provide valuable information for improving breeding programs and germplasm resource management (Roldán-Ruiz, 2001).…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat

Ejaz¹,

Zhu²,

Zhang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Amplified fragment length polymorphism markers were used to assess genetic diversity in 10 male sterile wheat crop lines (hetero-cytoplasm with the same nucleus) in relation to a restorer wheat line. These male sterile lines were evaluated using 64 amplified fragment length polymorphism primer combinations, and 13 primers produced polymorphic bands, generating a total 682 fragments. Of the 682 fragments, 113 were polymorphic. The polymorphic information content and marker index values demonstrated the utility of the primer combinations used in the present study. Unweighted pair group method with arithmetic mean and principal coordinate analysis of the genotypic data revealed clustering of accessions based on genetic relationships, and accessions were separated into 2 groups with their restorer line. M. Ejaz et al. 8936©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8935-8946 (2015) Jaccard's similarity coefficient values suggested good variability among the male sterile lines, indicating their utility in breeding programs. The fallouts of analysis of molecular variance showed large within-group population variation, accounting for 77% of variation, while amonggroup comparison accounted for 23% of the total molecular variation, which was statistically significant. The molecular diversity observed in this study will be useful for selecting appropriate accessions for plant improvement and hybridization through molecular-breeding approaches and for developing suitable conservation strategies.

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“…The alternative hypothesis that S. glynni is a relict population that has survived since the formation of the Isthmus of Panama´is unlikely, given previous ITS region rate estimates (standardized by excluding the 5.8S gene, rates are per nucleotide cite per million years), which vary several fold depending on the taxanomic group, e.g. ; 0.011-0.012 in Drosophilla (Schlotterer et al 1994), 0.004 in Birch trees (Savard et al 1993), 0.003625-0.00725 in Cucurbitaceae (Jobst et al 1998). Complete closure of the Central American Seaway was estimated at 3.8 MYA by Kegwin (1982), which would correspond to between 1.5% and 5% sequence divergence (assuming the range of previously published rates), which is considerably larger than the observed differences.…”

Section: Discussionmentioning

confidence: 99%

An ITS region phylogeny of Siderastrea (Cnidaria: Anthozoa): is S. glynni endangered or introduced?

et al. 2005

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The genus Siderastrea contains only five extant species, including Siderastrea glynni, which is one of the few recognized species of endangered stony coral. Cloned sequences of the internal transcribed spacer (ITS) region had low levels of intragenomic nucleotide diversity, and few alignment ambiguities, which allowed for the first species-level phylogenetic analysis of the genus. Results indicated an unexpected deep divergence between the Western-Pacific and Atlantic species. ITS region sequences indicated that S. glynni is not derived from S. savignyana, as previously thought. Instead, S. glynni shared identical sequence types with S. siderea in the Caribbean. Given a range of previously published evolutionary rates for the ITS region, it is unlikely that S. glynni represents the remnants of a population that was divided by the closure of the Central American Seaway (approximately 3-3.8 MYA). It is more likely that S. glynni originated by a breach of the Isthmus (approximately 2 MYA), or a contemporary introduction by ship.

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Molecular Evolution of the Internal Transcribed Spacers (ITS1 and ITS2) and Phylogenetic Relationships among Species of the Family Cucurbitaceae

Cited by 119 publications

References 63 publications

Seed coat diversity in some tribes of Cucurbitaceae: implications for taxonomy and species identification

Seed coat diversity in some tribes of Cucurbitaceae: implications for taxonomy and species identification

Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat

An ITS region phylogeny of Siderastrea (Cnidaria: Anthozoa): is S. glynni endangered or introduced?

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