Molecular evolution, virology and spatial distribution of HCV genotypes in Pakistan: A meta-analysis

Habib, Arslan; Habib, Nadiya; Anjum, Khalid Mahmood; Iqbal, Riffat; Ashraf, Zeeshan; Taj, Muhammad Usman; Asim, Muhammad; Javid, Kanwal; Idoon, Faezeh; Dashti, Saeid; Medeiros, Cassio Rocha; Gurgel, Ana Pavla Almeida Diniz; Coutinho, Henrique Douglas Melo

doi:10.1016/j.imj.2023.11.007

Cited by 2 publications

(2 citation statements)

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“…Similar results were found in other studies as well. Genotype 3 is endemic in Pakistan and other neighboring countries like India and Bangladesh 43 . While genotype 1a was found 2.7% in this study is highly prevalent in the United States 44 .…”

Section: Discussionmentioning

confidence: 99%

Determination of hepatitis C virus subtype prevalent in Sindh, Pakistan: a phylogenetic analysis

Farooq,

Faiz,

Wahab

et al. 2024

Sci Rep

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Hepatitis is a major public health issue, affecting 10–17 million people worldwide, with its prevalence continuously increasing. The Hepatitis C virus (HCV) is responsible for liver related diseases, which include liver cirrhosis, hepatocellular carcinoma, and chronic hepatitis. Pakistan is experiencing a serious rise in HCV cases. This study aimed to assess the prevalence and distribution of HCV genotypes in Sindh, Pakistan. Serum samples from HCV-positive patients were collected from various local hospitals in Sindh. These samples were first screened for HCV antibodies using ELISA. Samples that tested positive for HCV RNA underwent further genotyping through sequencing using the standard Sanger method. The genotypes were identified by comparing the sequences with those available in the National Center for Biotechnology Information (NCBI) database, and a phylogenetic tree was constructed. The phylogenetic analysis showed that all isolates in this study were clustered with genotypes 3a and 3b, except for one sequence that was clustered with genotype 1a. No isolates were found to be clustered with reference genomes of genotypes 2, 4, 5, 6, and 7 suggesting that genotype 3a is endemic in this region. The analyzed sequences demonstrated a 98% similarity with reference and isolated sequences. In summary, sequencing of the HCV 5′ UTR essential for identifying the predominant genotype of HCV RNA in the Sindh region Further research on the distribution of HCV genotypes in other regions of Pakistan could aid in improving screening processes, identifying more effective treatment options, and developing suitable prevention strategies.

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Section: Discussionmentioning

confidence: 99%

Determination of hepatitis C virus subtype prevalent in Sindh, Pakistan: a phylogenetic analysis

Farooq,

Faiz,

Wahab

et al. 2024

Sci Rep

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“…Globally, an estimated 70 million individuals grapple with the enduring affliction of hepatitis C virus (HCV) infection. 1,2 Directacting antiviral drugs (DAAs) have demonstrated remarkable efficacy in the treatment of Hepatitis C, not just in the general populace, but also in high-risk groups where they exhibit high rates of cure. [3][4][5] However, the lack of a durable immunological defense posttreatment leaves individuals vulnerable to reinfection.…”

Section: Introductionmentioning

confidence: 99%

Investigating the role of killer cell immunoglobulin‐like receptors and human leukocyte antigen genetic variants in hepatitis C virus infection

Li,

Zeng,

Huang

et al. 2024

Journal of Medical Virology

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The genetic diversity of killer cell immunoglobulin‐like receptors (KIRs) and human leukocyte antigen (HLA) genes influences the host's immune response to viral pathogens. This study aims to explore the impact of five single nucleotide polymorphisms (SNPs) in KIR3DL2 and HLA‐A genes on hepatitis C virus (HCV) infection. A total of 2251 individuals were included in the case‐control study. SNPs including KIR3DL2 rs11672983, rs3745902, rs1654644, and HLA‐A rs3869062, rs12202296 were genotyped. By controlling various confounding factors using a modified logistic regression model, as well as incorporating stratified analysis, joint effects analysis, and multidimensional bioinformatics analysis, we analyzed the relationship between SNPs and HCV infection. The logistic regression analysis showed a correlation between KIR3DL2 rs11672983 AA, KIR3DL2 rs3745902 TT, and increased HCV susceptibility (p < 0.01). Stratified analysis indicated that KIR3DL2 rs1654644 and HLA‐A rs3869062 also heightened HCV susceptibility in certain subgroups. A linear trend of rising HCV infection rates was observed when combining KIR3DL2 rs11672983 AA and KIR3DL2 rs3745902 TT (ptrend = 0.007). Bioinformatics analysis suggested these SNPs' regulatory potential and their role in altering messenger RNA secondary structure, implying their functional relevance in HCV susceptibility. Our findings indicate that KIR3DL2 rs11672983 AA and KIR3DL2 rs3745902 TT are significantly associated with increased susceptibility to HCV infection.

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Molecular evolution, virology and spatial distribution of HCV genotypes in Pakistan: A meta-analysis

Cited by 2 publications

References 50 publications

Determination of hepatitis C virus subtype prevalent in Sindh, Pakistan: a phylogenetic analysis

Determination of hepatitis C virus subtype prevalent in Sindh, Pakistan: a phylogenetic analysis

Investigating the role of killer cell immunoglobulin‐like receptors and human leukocyte antigen genetic variants in hepatitis C virus infection

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