Molecular evolutionary genetics of the cattle-adapted serovar Salmonella dublin

Selander, Robert K.; Smith, Noel H.; Li, J; Beltran, P.; Ferris, Kathleen E.; Kopecko, Dennis J.; Rubin, Fran A.

doi:10.1128/jb.174.11.3587-3592.1992

Cited by 86 publications

(80 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The three remaining molecular variants were each recognized in single strains from different countries and none had diverged greatly from the reference SSP pOG675. Together these data suggest the possibility that co-resident plasmids may have influenced the evolution of the SSP in Dublin and go some way to explain why the SSPs of Dublin and Enteritidis are more markedly divergent than the genomic DNA appears to be on the basis of MLEE [23] and IS200 fingerprinting [25].…”

Section: Discussionmentioning

confidence: 85%

“…Population genetic studies of Dublin [23] based on multilocus enzyme electrophoresis (MLEE) distinguished 4 clones, 3 of which were closely related (Dul, Du3 and Du4); Dul had a world-wide distribution, Du3 was restricted to the UK and France and Du4 was unique to the USA. The distribution of plasmids within Dul was limited to either the SSP alone or the SSP together with a single small (3 [23].…”

Section: Discussionmentioning

confidence: 99%

“…The distribution of plasmids within Dul was limited to either the SSP alone or the SSP together with a single small (3 [23].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

1995

View full text Add to dashboard Cite

SUMMARYMolecular variation within and between plasmids ofSalmonella enterica serotype Dublin was analysed. Such variation has been demonstrated in the serotypespecific plasmids (SSP's) of Typhimurium and Enteritidis. The two aims of this study were to determine the plasmid diversity in a host-adapted serotype and also the incidence of molecular variation in the SSP among strains of Dublin using restriction endonuclease fragmentation pattern (REFP) analysis with Pstl, Smal and EcoRV. Sixty-five strains were examined from seven countries. Plasmid profile and REFP analysis showed that none of the strains was plasmid-free. Seventy-seven percent of the strains possessed the 72 kb SSP either alone or in combination with another plasmid; 23 % harboured plasmids which were molecular variants of the SSP. Four of the variants were more closely related to each other than to the reference SSP and were harboured by Dublin isolated from both the USA and Europe. A further three were shown to be cointegrate plasmids and were similarly distributed. Thirty-two percent of strains possessed the SSP alone. None of the UK strains was resistant to any of the antimicrobial agents tested whereas 74 % of the remaining strains were resistant to between one and five antimicrobial agents. This study corroborates previous findings concerning the high degree of stability of the SSP and confirmed the clonal nature of Dublin.Co-resident plasmids provided evidence of sub-clones within localized geographical areas.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

1995

View full text Add to dashboard Cite

show abstract

“…Dublin consists of three closely related electrophoretic types with 95 % of strains belonging to a clone that is present world-wide [39,40]. Dublin strains showed the same RFLP-patterns when hybridized with the insertion sequence IS200 while restriction polymorphism can be demonstrated by ribotyping [41].…”

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

Host adapted serotypes of Salmonella enterica

et al. 2000

View full text Add to dashboard Cite

Salmonella constitutes a genus of zoonotic bacteria of worldwide economic and health importance. The current view of salmonella taxonomy assigns the members of this genus to two species: S. enterica and S. bongori. S. enterica itself is divided into six subspecies, enterica, salamae, arizonae, diarizonae, indica, and houtenae, also known as subspecies I, II, IIIa, IIIb, IV, and VI, respectively [1]. Members of Salmonella enterica subspecies enterica are mainly associated with warm-blooded vertebrates and are usually transmitted by ingestion of food or water contaminated by infected faeces. The pathogenicity of most of the distinct serotypes remains undefined, and even within the most common serotypes, many questions remain to be answered regarding the interactions between the organism and the infected host.Salmonellosis manifests itself in three major forms: enteritis, septicaemia, and abortion, each of which may be present singly or in combination, depending on both the serotype and the host involved. Although currently over 2300 serovars of Salmonella are recognized, only about 50 serotypes are isolated in any significant numbers as human or animal pathogens [2, 3] and they all belong to subspecies enterica. Of these, most cause acute gastroenteritis characterized by a short incubation period and a severe systemic disease in man or animals, characterized by septicaemia, fever and/or abortion, and such serotypes are often associated with one or few host species [4–6].It is the intention of this review to present a summary of current knowledge of these host-adapted serotypes of S. enterica. The taxonomic relationships between the serotypes will be discussed together with a comparison of the pathology and pathogenesis of the disease that they cause in their natural host(s). Since much of our knowledge on salmonellosis is based on the results of work on Typhimurium, this serotype will often be used as the baseline in discussion. It is hoped that an appreciation of the differences that exist in the way these serotypes interact with the host will lead to a greater understanding of the complex host–parasite relationship that characterizes salmonella infections.

show abstract

“…The virulence capsular polysaccharide (Vi antigen), a homopolymer of N-acetylgalactosamine uronic acid that forms a coat on the external surface of the bacterial cell, is expressed by S. Typhi, S. Paratyphi C, some of the strains of S. Dublin, and a few strains of Citrobacter freundii [20]. Production of the Vi antigen is controlled by two chromosomal loci, viaA and viaB.…”

mentioning

confidence: 99%

Application of PCR-based serogrouping of selected Salmonella serotypes in Malaysia

Lim

Thong

2009

J Infect Dev Ctries

View full text Add to dashboard Cite

Background: Differentiation of Salmonella enterica into its serogroups is important for epidemiological study. The objective of the study was to apply a multiplex PCR targeting serogroups A, B, C1, D, E and Vi-positive strains of Salmonella enterica commonly found in Malaysia. A separate H-typing multiplex PCR which identified flagellar antigen "a", "b" or "d" was also optimized to confirm clinical serotypes, S. Paratyphi A and S. Typhi. Methodology: Sixty-seven laboratory Salmonella enterica strains were tested. Six sets of primers targeting defined regions of the O antigen synthesis genes (rfb gene cluster) and Vi antigen gene (viaB) were selected and combined into a multiplex PCR for O-grouping. Four primers (H-for, Ha-rev, Hb-rev and Hd-rev) were used in the second step multiplex PCR for H-typing. The optimized mPCR assays were further evaluated with 58 blind-coded Salmonella strains. Results: The multiplex PCR results obtained showed 100% concordance to the conventionally typed serogroups. Validation with 58 blind coded Salmonella strains yield 100% accuracy and specificity. Conclusion: Based on this study, PCR serogrouping proved to be a rapid, alternative method for further differentiation of Salmonella enterica.

show abstract

Molecular evolutionary genetics of the cattle-adapted serovar Salmonella dublin

Cited by 86 publications

References 43 publications

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

Host adapted serotypes of Salmonella enterica

Application of PCR-based serogrouping of selected Salmonella serotypes in Malaysia

Contact Info

Product

Resources

About