Molecular expression of PsPIN1, a putative auxin efflux carrier gene from pea (Pisum sativum L.)

Chawla, Rekha; DeMason, Darleen A.

doi:10.1007/s10725-004-2139-9

Cited by 30 publications

(33 citation statements)

References 77 publications

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“…As some genes in the AS2/LBD family are induced by auxin in an ARF-dependent manner in Arabidopsis and the overexpression of LBD16 promotes lateral root formation in the arf7 arf19 double mutant [110], rice and Arabidopsis appear to share at least some downstream targets for ARFs. Regulation of PIN-gene expression appears to be shared between Arabidopsis and pea, as accumulation of transcripts of a pea homologue of PIN gene (PsPIN1) is upregulated by auxin [140]. In summary, increasing lines of evidence suggest that dicotyledonous and monocotyledonous plants share at least some key components for light and gravity perception, auxin transport and downstream factors.…”

Section: Pat In Non-arabidopsis Speciesmentioning

confidence: 99%

Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development

Tanaka

Dhonukshe

Brewer

et al. 2006

Cell. Mol. Life Sci.

329

291

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The plant hormone auxin plays crucial roles in regulating plant growth development, including embryo and root patterning, organ formation, vascular tissue differentiation and growth responses to environmental stimuli. Asymmetric auxin distribution patterns have been observed within tissues, and these so-called auxin gradients change dynamically during different developmental processes. Most auxin is synthesized in the shoot and distributed directionally throughout the plant. This polar auxin transport is mediated by auxin influx and efflux facilitators, whose subcellular polar localizations guide the direction of auxin flow. The polar localization of PIN auxin efflux carriers changes in response to developmental and external cues in order to channel auxin flow in a regulated manner for organized growth. Auxin itself modulates the expression and subcellular localization of PIN proteins, contributing to a complex pattern of feedback regulation. Here we review the available information mainly from studies of a model plant, Arabidopsis thaliana, on the generation of auxin gradients, the regulation of polar auxin transport and further downstream cellular events.

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Section: Pat In Non-arabidopsis Speciesmentioning

confidence: 99%

Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development

Tanaka

Dhonukshe

Brewer

et al. 2006

Cell. Mol. Life Sci.

329

291

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“…If either auxin transport (by NPA or TIBA) Chawla 2004a, 2004b) or GA biosynthesis is inhibited or reduced (this study) the leaf phenotypes produced are similar to that of the uni and uni-tac mutants. The mRNA levels of two pea genes important to PAT, PsPIN1 and PsPK2 (= PID) are up-regulated by both auxin and GA (Chawla and DeMason 2004;Bai et al 2005). Since the PIN protein is thought to be a component of the auxin efflux transporter and PID regulates its targeting, the possibility exists that PAT in pea leaves is also regulated by both hormones.…”

Section: Role Of Gibberellin In Pea Leaf Morphogenesismentioning

confidence: 99%

Auxin–cytokinin and auxin–gibberellin interactions during morphogenesis of the compound leaves of pea (Pisum sativum)

DeMason

2005

Planta

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A number of mutations that alter the form of the compound leaf in pea (Pisum sativum) has proven useful in elucidating the role that auxin might play in pea leaf development. The goals of this study were to determine if auxin application can rescue any of the pea leaf mutants and if gibberellic acid (GA) plays a role in leaf morphogenesis in pea. A tissue culture system was used to determine the effects of various auxins, GA or a GA biosynethesis inhibitor (paclobutrazol) on leaf development. The GA mutant, nana1 (na1) was analyzed. The uni-tac mutant was rescued by auxin and GA and rescue involved both a conversion of the terminal leaflet into a tendril and an addition of a pair of lateral tendrils. This rescue required the presence of cytokinin. The auxins tested varied in their effectiveness, although methyl-IAA worked best. The terminal tendrils of wildtype plantlets grown on paclobutrazol were converted into leaflets, stubs or were aborted. The number of lateral pinna pairs produced was reduced and leaf initiation was impaired. These abnormalities resembled those caused by auxin transport inhibitors and phenocopy the uni mutants. The na1 mutant shared some morphological features with the uni mutants; including, flowering late and producing leaves with fewer lateral pinna pairs. These results show that both auxin and GA play similar and significant roles in pea leaf development. Pea leaf morphogenesis might involve auxin regulation of GA biosynthesis and GA regulation of Uni expression.

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“…While the complex signal transduction cascade regulating the differential cell elongation that causes gravitropic and phototropic responses remains unclear, a possible role for auxin in tropic bending has been proposed by the Cholodny-Went hypothesis, which contends that unequal distribution of auxin between the opposite sides of an organ causes differential cell elongation (Went 1974). Recently, we demonstrated a characteristic asymmetrical polar auxin movement that is gravity-controlled in the early growth stages of etiolated pea epicotyls, and suggested its importance for inducing asymmetrical accumulation of auxin during the negative gravitropic response of the epicotyls, based on the results of transport experiments using radiolabeled auxin and gene expression analyses of PsPIN1 , which is considered to be an auxin efflux facilitator protein (Chawla and DeMason 2004;Hoshino et al 2005;Bai and DeMason 2006).…”

Section: Introductionmentioning

confidence: 99%

Gravity-controlled asymmetrical transport of auxin regulates a gravitropic response in the early growth stage of etiolated pea (Pisum sativum) epicotyls: studies using simulated microgravity conditions on a three-dimensional clinostat and using an agravitropic mutant, ageotropum

2007

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Increased expression of the auxin-inducible gene PsIAA4/5 was observed in the elongated side of epicotyls in early growth stages of etiolated pea (Pisum sativum L. cv. Alaska) seedlings grown in a horizontal or an inclined position under 1 g conditions. Under simulated microgravity conditions on a 3D clinostat, accumulation of PsIAA4/5 mRNA was found throughout epicotyls showing automorphosis. Polar auxin transport in the proximal side of epicotyls changed when the seedlings were grown in a horizontal or an inclined position under 1 g conditions, but that under clinorotation did not, regardless of the direction of seed setting. Accumulation of PsPIN1 and PsPIN2 mRNAs in epicotyls was affected by gravistimulation, but not by clinorotation. Under 1 g conditions, auxin-transport inhibitors made epicotyls of seedlings grown in a horizontal or inclined position grow toward the proximal direction to cotyledons. These inhibitors led to epicotyl bending toward the cotyledons in seedlings grown in an inclined position under clinorotation. Polar auxin transport, as well as growth direction, of epicotyls of the agravitropic mutant ageotropum did not respond to various gravistimulation. These results suggest that alteration of polar auxin transport in the proximal side of epicotyls regulates the graviresponse of pea epicotyls.

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Molecular expression of PsPIN1, a putative auxin efflux carrier gene from pea (Pisum sativum L.)

Cited by 30 publications

References 77 publications

Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development

Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development

Auxin–cytokinin and auxin–gibberellin interactions during morphogenesis of the compound leaves of pea (Pisum sativum)

Gravity-controlled asymmetrical transport of auxin regulates a gravitropic response in the early growth stage of etiolated pea (Pisum sativum) epicotyls: studies using simulated microgravity conditions on a three-dimensional clinostat and using an agravitropic mutant, ageotropum

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