Molecular farming in plants: Oilseeds as vehicles for the production of pharmaceutical proteins

Boothe, J. G.; Saponja, Jillian A.; Parmenter, D. L.

doi:10.1002/(sici)1098-2299(199711/12)42:3/4<172::aid-ddr9>3.0.co;2-n

Cited by 52 publications

(25 citation statements)

References 56 publications

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“…Several biotechnological applications have been developed by using the unique characteristics of oil bodies, such as new ingredients for flavoring or emulsifying agents, affinity matrices for enzyme fixation/purification, and expression/purification systems for producing recombinant proteins via transgenic plants [144][145][146][147][148][149][150][151][152][153][154]. Many applications related to the utilization of oil bodies have been patented [155][156][157][158][159][160][161][162][163].…”

Section: Artificial Oil Bodies In Biotechnological Applicationsmentioning

confidence: 99%

Integral Proteins in Plant Oil Bodies

Tzen

2012

ISRN Botany

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Hydrophobic storage neutral lipids are stably preserved in specialized organelles termed oil bodies in the aqueous cytosolic compartment of plant cells via encapsulation with surfactant molecules including phospholipids and integral proteins. To date, three classes of integral proteins, termed oleosin, caleosin, and steroleosin, have been identified in oil bodies of angiosperm seeds. Proposed structures, targeting traffic routes, and biological functions of these three integral oil-body proteins were summarized and discussed. In the viewpoint of evolution, isoforms of oleosin and caleosin are found in oil bodies of pollens as well as those of more primitive species; moreover, caleosin-and steroleosin-like proteins are also present in other subcellular locations besides oil bodies. Technically, artificial oil bodies of structural stability similar to native ones were successfully constituted and seemed to serve as a useful tool for both basic research studies and biotechnological applications.

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Section: Artificial Oil Bodies In Biotechnological Applicationsmentioning

confidence: 99%

Integral Proteins in Plant Oil Bodies

Tzen

2012

ISRN Botany

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“…Hence, further studies are necessary to determine the cause of denaturation. It is possible that the denaturation is related to the choice of surfactant or His [protein] initial volume initial (2) protein; additional surfactants and His proteins will be considered in the future.…”

Section: Resultsmentioning

confidence: 99%

“…Of the systems available for the large-scale production of recombinant proteins, the use of transgenic plants is potentially one of the most economical (1,2). Although there has been much success in the area of transgenic plant development and gene-expression systems (1), several downstream processing issues could be further improved to decrease processing costs and facilitate the wide spread commercial use of these plants as bioreactors (2,3).…”

Section: Introductionmentioning

confidence: 99%

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Histidine Tagged Protein Recovery from Tobacco Extract by Foam Fractionation

Crofcheck

Loiselle

Weekley

et al. 2003

Biotechnology Progress

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Tobacco plants have the potential to be used for the production of proteins for pharmaceutical applications. This work describes a novel protein recovery strategy where the protein of interest is "tagged" with a histidine sequence, which forms a complex with cobalt ions and surfactant possessing a chelating functionality, such that the protein is recovered in the foamate of a foam fractionation step. His-gus, a histidine-tagged enzyme, was chosen as a model protein to study the feasibility of this strategy. The His-gus is recovered from spiked prefoamed tobacco extract by foam fractionation in the presence of surfactant and cobalt ions with an enrichment of 1.29 and a recovery of 21.5% in terms of an adjusted activity.

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“…It has been reported that an expression level of up to 30% of total soluble protein for an animal vaccine can be produced in transgenic tobacco plants [18]. Other plants have also been used, including rice [11,19,20], wheat [20], maize [21] and oilseed rape [22][23][24], since it is considered that full-scale commercial production could involve grain and oilseed crops. The production of vaccines, antibodies and biopharmaceuticals in transgenic plants has been summarized in a previous review article [14].…”

Section: New Effective Means For Rice Growth In Plant Factoriesmentioning

confidence: 99%

Feasibility Study of Rice Growth in Plant Factories

Yamori¹,

Zhang²

2014

J Rice Res

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Global food production will need to increase more than 50% before 2050 to satisfy the food demands of an increasing human population. To solve this food crisis, an increase in rice production will be necessary as rice is especially vital for the nutrition of much of the population in many countries. One solution to the future food shortage worldwide would be to cultivate crops in places where plants to do typically grow (e.g., desert and Antarctic). The technology for "plant factories", a new facility to grow plants under a controlled environment that would enable high yield and high quality production year-round, is progressing. Through the computer-based, automated control of environmental conditions for optimal rice growth, the external environmental conditions would have little effect on the plants growing inside the plant factory. In the near future, the plant factory could play an important role in areas where field productions are difficult or impossible. Moreover, since producing biopharmaceuticals in plants through genetic engineering technologies has many economic and qualitative benefits, the trial to produce useful materials in transgenic plants is examined in the plant factory. Transgenic plants might therefore expand the usefulness of plant factories. In this review article, feasibility of rice growth in plant factories is summarized.

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Molecular farming in plants: Oilseeds as vehicles for the production of pharmaceutical proteins

Cited by 52 publications

References 56 publications

Integral Proteins in Plant Oil Bodies

Integral Proteins in Plant Oil Bodies

Histidine Tagged Protein Recovery from Tobacco Extract by Foam Fractionation

Feasibility Study of Rice Growth in Plant Factories

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