Molecular features ofMycobacterium aviumhuman isolates carrying a single copy of IS1245and IS1311per genome

Murcia, Marta; García, María Jesús García; Otal, Isabel; Gómez, Alberto Gómez; Menéndez, M. Carmen

doi:10.1111/j.1574-6968.2007.00769.x

Cited by 2 publications

(2 citation statements)

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“…The insertion of an IS within another IS is not a rare event, it has been described in M. tuberculosis [25] and other bacteria, such as Pseudomonas syringae [26] , Rhizobium meliloti [27] and Escherichia coli K-12 [28] . It has been previously shown that the genomic insertion loci of IS 1245 and IS 1311 , in M. avium strains carrying a single copy of any of these insertion sequences, are located in areas containing putatively truncated integrases and/or transposases [29] . Likewise, the transposase-related sequence identified in pMA100 could be a preferential locus for insertion of IS 1245 .…”

Section: Discussionmentioning

confidence: 99%

First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid

et al. 2012

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BackgroundIn a previous study, we detected the presence of a Mycobacterium avium species-specific insertion sequence, IS1245, in Mycobacterium kansasii. Both species were isolated from a mixed M. avium-M. kansasii bone marrow culture from an HIV-positive patient. The transfer mechanism of this insertion sequence to M. kansasii was investigated here.Methodology/Principal FindingsA linear plasmid (pMA100) was identified in all colonies isolated from the M. avium-M. kansasii mixed culture carrying the IS1245 element. The linearity of pMA100 was confirmed. Other analyses suggested that pMA100 contained a covalently bound protein in the terminal regions, a characteristic of invertron linear replicons. Partial sequencing of pMA100 showed that it bears one intact copy of IS1245 inserted in a region rich in transposase-related sequences. These types of sequences have been described in other linear mycobacterial plasmids. Mating experiments were performed to confirm that pMA100 could be transferred in vitro from M. avium to M. kansasii. pMA100 was transferred by in vitro conjugation not only to the M. kansasii strain from the mixed culture, but also to two other unrelated M. kansasii clinical isolates, as well as to Mycobacterium bovis BCG Moreau.Conclusions/SignificanceHorizontal gene transfer (HGT) is one of most important mechanisms leading to the evolution and diversity of bacteria. This work provides evidence for the first time on the natural occurrence of HGT between different species of mycobacteria. Gene transfer, mediated by a novel conjugative plasmid, was detected and experimentally reproduced.

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Section: Discussionmentioning

confidence: 99%

First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid

et al. 2012

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“…In 4H2, the transposon inserted within the intergenic region between MAP1150c and MAP1151c. MAP1150c is the transposase for IS 1311 (Murcia et al, 2007 ), an insertion sequence present at eight copies in the MAP genome (Li et al, 2005 ). Thus, any effect of this insertion on the MAP1150c is not likely to have a major impact on MAP physiology.…”

Section: Discussionmentioning

confidence: 99%

Generation and screening of a comprehensive Mycobacterium avium subsp. paratuberculosis transposon mutant bank

Rathnaiah

Lamont

Harris

et al. 2014

Front. Cell. Infect. Microbiol.

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Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne's Disease in ruminants. This enteritis has significant economic impact and worldwide distribution. Vaccination is one of the most cost effective infectious disease control measures. Unfortunately, current vaccines reduce clinical disease and shedding, but are of limited efficacy and do not provide long-term protective immunity. Several strategies have been followed to mine the MAP genome for virulence determinants that could be applied to vaccine and diagnostic assay development. In this study, a comprehensive mutant bank of 13,536 MAP K-10 Tn5367 mutants (P > 95%) was constructed and screened in vitro for phenotypes related to virulence. This strategy was designated to maximize identification of genes important to MAP pathogenesis without relying on studies of other mycobacterial species that may not translate into similar effects in MAP. This bank was screened for mutants with colony morphology alterations, susceptibility to D-cycloserine, impairment in siderophore production or secretion, reduced cell association, and decreased biofilm and clump formation. Mutants with interesting phenotypes were analyzed by PCR, Southern blotting and DNA sequencing to determine transposon insertion sites. These insertion sites mapped upstream from the MAP1152-MAP1156 cluster, internal to either the Mod operon gene MAP1566 or within the coding sequence of lsr2, and several intergenic regions. Growth curves in broth cultures, invasion assays and kinetics of survival and replication in primary bovine macrophages were also determined. The ability of vectors carrying Tn5370 to generate stable MAP mutants was also investigated.

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Molecular features ofMycobacterium aviumhuman isolates carrying a single copy of IS1245and IS1311per genome

Cited by 2 publications

References 41 publications

First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid

First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid

Generation and screening of a comprehensive Mycobacterium avium subsp. paratuberculosis transposon mutant bank

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