Molecular fingerprinting and assessment of genetic variations among advanced breeding lines of Moringa oleifera L. by using seed protein, RAPD and Cytochrome P450 based markers

Kumar, Pardeep; Dolkar, Rinchan; Manjunatha, Girigowda; Pallavi, H.M.

doi:10.1016/j.sajb.2017.03.024

Cited by 13 publications

(10 citation statements)

References 22 publications

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“…Therefore, for the collection of seeds and for the seedling production which are both aimed at enriching the area, all of the individuals were important. These strategies have been considered and they have been supported by a study on Moringa oleifera L, where the estimated similarities, by the Jaccard coefficient, varied from 0.248 to 0.889 (Kumar et al, 2017). Saini et al (2013) found a similar results.…”

Section: Resultsmentioning

confidence: 81%

Forest inventory and the genetic diversity of the remaining fragment of Hymenaea courbaril L.

et al. 2018

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Hymenaea courbaril is a rare species in the riparian areas of the state of Sergipe, Brazil. This species is known as Brazilian cherry or Brazilian copal and it occurs in fragments of the Rainy Forest and in the transition zones between the Rainy Forest and the Caatinga Biomes. This work was carried out, in order to quantify the genetic diversity of the remaining population of Brazilian cherry, by RAPD markers. In a studied area of 100 ha in the low course of the São Francisco River, only 15 individuals were observed. The analyzed parameters were as follows: Jaccard’s genetic similarity, the number of observed alleles, the number of effective alleles, the genetic diversities of Nei, the percentages of the polymorphic loci, the genetic diversities when using the Shannon index and the coancestry coefficient. There was no correlation between the genetic distances and the geographical distances. Despite the low number of individuals, high genetic diversity was observed, a fact that contributes to the development of strategies for the conservation of the species.

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Section: Resultsmentioning

confidence: 81%

Forest inventory and the genetic diversity of the remaining fragment of Hymenaea courbaril L.

et al. 2018

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“…A possible explanation for the lack of correlation between genetic variability versus geographic origin can be explained by the origin center of moringa. Most genetic studies with moringa are performed in Asia, more speci cally in India, its origin center (Muluvi et al 1999;Saini et al 2013;Ganesan et al 2014;Kumar et al 2017;Ravi et al 2020). In addition, studies with cultivated and natural accessions to quantify the genetic diversity of moringa across the world are considered meagre, although the conservation of genetic resources for this species by using germplasm banks is increasing (Boopathi et al 2021).…”

Section: Resultsmentioning

confidence: 99%

Moringa oleifera Genebank in Brazil: current status and future approaches

Soares,

Gois,

Souza

et al. 2023

Preprint

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Moringa oleifera Lam. is a tropical tree that belongs to the Moringaceae family, and it is popularly known worldwide for its multiple applications. This study aimed to evaluate the genetic variability of individuals from the Moringa Genebank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. The Moringa Genebank is composed of 25 accessions, represented by 177 genotypes, of which 18 were transferred from an exchanged germplasm of the University of Florida, USA, and the others were from different states of Brazil. Leaves of each genotype were collected for DNA extraction and PCR analysis using 20 ISSR primers. A total of 144 bands were amplified and 100% of them were polymorphic. The average of expected heterozygosity (He) and Shannon’s Index was 0.11 and 0.12, respectively. The highest genetic divergence was found between M4 and M18 accessions, both from Florida, USA. Whereas the closest pair of accession was M23 and M24, both from Brazil. The cluster analysis obtained through the Structure software divided moringa genotypes into two groups. Taken together, these results suggest low genetic diversity between the accessions of the Moringa Genebank. Therefore, the introduction of new accessions in the Moringa GeneBank is essential to increase the genetic variability of the species to ensure its conservation and improvement.

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“…Antioxidants (β-carotene and vitamins A, C, and E), biochemicals (amino acids, chlorophyll, glucosinolates, seed protein, sugars, and total protein), heavy metals, in vitro gas production, macronutrients (calcium [Ca], magnesium [Mg], nitrogen [N], phosphorus [P], and potassium [K]), micronutrients (copper [Cu], iron [Fe], manganese [Mn], and zinc [Zn]), nutritional and anti-nutritional factors (lead [Pb], oligosaccharides, and oxalate), and polyphenols (baicalin, caffeic acid, chlorogenic acid, cinnamic acid, coumaric acid, ferulic acid, gallic acid, gallogen, isoquercetin, kaempferide, quercetin, quercitrin, rutin, and vanillin) have been employed for determining the genetic variability among the accessions and/or advanced breeding lines of MO from China, India, Laos, Philippines, Saudi Arabia, Taiwan, Tanzania, Thailand, and the USA [32,[54][55][56][57][58][59] (Table 1). However, like morphological markers, biochemical markers also have several limitations, i.e., their fewer numbers, low efficacy in detecting polymorphism and being affected by the growth and developmental stages of the plant, and various biotic and abiotic stresses [36].…”

Section: Biochemicals Nutritional and Anti-nutritional Elementsmentioning

confidence: 99%

Miracle Tree Moringa oleifera: Status of the Genetic Diversity, Breeding, In Vitro Propagation, and a Cogent Source of Commercial Functional Food and Non-Food Products

Alavilli

Yugandhar

Verma

et al. 2022

Plants

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Moringa oleifera Lam. (MO) is a fast-growing drought-resistant tree belonging to the family Moringaceae and native to the Indian subcontinent and cultivated and/or naturalized worldwide with a semi-arid climate. MO is also popularly known as a miracle tree for its repertoire of nutraceutical, pharmacological, and phytochemical properties. The MO germplasm is collected, conserved, and maintained by various institutions across the globe. Various morphological, biochemical, and molecular markers are used for determining the genetic diversity in MO accessions. A higher yield of leaves and pods is often desirable for making various products with commercial viability and amenable for trade in the international market. Therefore, breeding elite varieties adapted to local agroclimatic conditions and in vitro propagation are viable and sustainable approaches. Here, we provide a comprehensive overview of MO germplasm conservation and various markers that are employed for assessing the genetic diversity among them. Further, breeding and in vitro propagation of MO for various desirable agronomic traits are discussed. Finally, trade and commerce of various functional and biofortified foods and non-food products are enumerated albeit with a need for a rigorous and stringent toxicity evaluation.

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Molecular fingerprinting and assessment of genetic variations among advanced breeding lines of Moringa oleifera L. by using seed protein, RAPD and Cytochrome P450 based markers

Cited by 13 publications

References 22 publications

Forest inventory and the genetic diversity of the remaining fragment of Hymenaea courbaril L.

Forest inventory and the genetic diversity of the remaining fragment of Hymenaea courbaril L.

Moringa oleifera Genebank in Brazil: current status and future approaches

Miracle Tree Moringa oleifera: Status of the Genetic Diversity, Breeding, In Vitro Propagation, and a Cogent Source of Commercial Functional Food and Non-Food Products

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