Molecular fingerprinting methods for the discrimination between C. albicans and C. dubliniensis

Abstract

Opportunistic fungal pathogens are becoming increasingly important causes of both community-acquired and nosocomial infections. The most important fungal pathogens are yeast species belonging to the genus Candida. These species show differences in levels of resistance to antifungal agents and mortality. Consequently, it is important to correctly identify the causative organism to the species level. Identification of Candida dubliniensis in particular remains problematic because of the high degree of phenotypic similarity between this species and Candida albicans. However, as the differences between both are most pronounced at the genetic level, several studies have been conducted in order to provide a specific and rapid identification fingerprinting molecular test. In most candidal infectious, no single DNA fingerprinting technique has evolved as a dominant method, and each method has its advantages, disadvantages and limitations. Moreover, the current challenge of these techniques is to compile standardized patterns in a database for interlaboratory use and future reference. This review provides an overview of most common molecular fingerprinting techniques currently available for discrimination of C. albicans and C. dubliniensis.

Identification of medicinal plants effective in infectious diseases in Urmia, northwest of Iran

“…In recent years, yeasts and particularly candidate species have emerged as one of the most common pathogens isolated from human infections [7] .…”

Comparison Between Phenotypic Characterization and Semi-Nested PCR for the Identification of Candida Species in Clinical Isolates

“…Nowadays, despite progresses in therapeutic methods and sanitary cares, the incidence of invasive systemic mycoses, especially opportunistic fungal pathogens such as Candida, is on the increase (14). Although C. albicans is the most frequent cause of candidiasis, the incidence of the disease caused by other species of Candida has been increasing drastically (11,14).…”

“…Although C. albicans is the most frequent cause of candidiasis, the incidence of the disease caused by other species of Candida has been increasing drastically (11,14). Among the azoles, fluconazole has revealed tolerance; and antifungal drug resistance is becoming a major problem, especially in immunocompromised patients (15,16).…”

Diagnostic Techniques of Oral Candidosis: A Review

“…This is a challenging problem because of the high degree of similarity in the phenotype between C. dubliniensis and C. Albicans 9 . Multiplex PCR has been used to identify various Candida species, particularly C. Albicans, C. glabrata, and C. tropicalis in a single sample 20 .…”