Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

Powe, Aleeta M.; Fletcher, Kristin A.; Luce, Nadia N. St.; Lowry, Mark; Neal, Sharon L.; McCarroll, Matthew E.; Oldham, Philip B.; McGown, Linda B.; Warner, Isiah M.

doi:10.1021/ac040095d

Cited by 71 publications

(42 citation statements)

References 191 publications

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“…The fluorescence increase of compounds in RTILs were first reported in 2006 34 , As shown in Figure 2, an methanol solution of curcumin had weak fluorescence; however, increases in fluorescence intensity was observed when it was excited in [ 6 ] was used as the extraction solvent. Under optimal experimental conditions, the extraction recovery rate was 98.1% and the excitation and emission of the blank reagent do not affect the determination of curcumin.…”

Section: Ils Sensitized Fluorescencementioning

confidence: 92%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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A new ionic liquid (IL) based micro-extraction method was developed for the spectrophotometry and spectrofluorometry determination of curcumin in curry products. ILs can effectively extract curcumin from aqueous solution which were used instead of large-scale organic solvents to reduce environmental pollution and production cost. Results show that the fluorescence of curcumin is considerably enhanced in 1-hexyl-3-methylimidazolium hexafluorophosphate ([Hmim][PF 6 ]). Under optimized conditions, the absorbance of curcumin is proportional to the concentration of curcumin in the range of 18.4 ng/mL to 494 ng/mL, and the detection limit is 6.1 ng/mL. In addition, the fluorescence intensity of curcumin is proportional to the concentration of curcumin in the range of 0.050 ng/mL to 120.1 ng/mL, and the detection limit is 0.002 ng/mL. Therefore, an efficient spectrophotometry and spectrofluorometry method for the determination of curcumin was established, and the actual concentration in curry products was satisfactorily determined. The content of curcumin in curry sauce were 14.10±0.40 and 14.32±0.37 mg/g when using the spectrophotometry and spectrofluorometry, the content of curcumin in curry powder were 2.50±1.73 and 2.75±1.40 mg/g when using the spectrophotometry and spectrofluorometry.

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Section: Ils Sensitized Fluorescencementioning

confidence: 92%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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“…In this technique, for example, the electrophoresis should be conducted with the usage of non-denatured gel and running at low temperature to keep the proteins active; otherwise CL signal cannot be seen. CL-imaging methods offer many advantages for high-throughput evaluation, high sensitivity, simple instrumentation, and exclusion of the usage of radioisotopes [8,9]. We reported a CL immunoassay for the sensitive detection of a CYP3A4 protein utilizing two antibodies and a dextran-luminol-biotin (DLB) polymer [10].…”

Section: Introductionmentioning

confidence: 99%

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Zhang¹,

Shibata²,

Krawczyk³

et al. 2009

Talanta

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Facile and non-radioactive methods are desired for the sensitive detection and quantification of various proteins. Herein we describe a novel chemiluminescence (CL)-detection method of particular proteins based on direct binding of a dextran-luminol-biotin (DLB) CL polymer to the proteins on a poly(vinylidene difluoride) membrane. Among 32 kinds of the proteins screened, several proteins such as drug-metabolizing enzymes, cytochrome p450 (CYP)1A2, CYP2E1, and CYP3A4 had the ability to bind directly to the DLB polymer. The binding site in the polymer was owing to the framework of the modified dextran, which underwent oxidation and reduction procedures. This interaction might be the comprehensive effect of both electrostatic interaction and steric complementarities. CL intensity of the proteins detected by the polymer could be further enlarged by the mediation of avidin. The proposed CL-imaging method possesses potential as a rapid, facile, inexpensive and selective detection of the proteins.

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“…Several thorough review papers have been published recently on analytical CL [69,70], its biotechnical and clinical applications [71,72], analytical ECL [73], and luminescence methods generally [74,75]. Also, CL detection for CE and LC was recently reviewed [76], along with ECL detection for microfluidic chips [77] and, more specifically, the current status of tris-(2,2'-bipyridyl) ruthenium (II) (Ru(bpy) 3 21 ) and its derivatives for ECL detection [78].…”

Section: Chemiluminescence and Electrochemiluminescence Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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The direction of modern analytical techniques is to push for lower detection limits, improved selectivity and sensitivity, faster analysis time, higher throughput, and more inexpensive analysis systems with ever-decreasing sample volumes. These very ambitious goals are exacerbated by the need to reduce the overall size of the device and the instrumentation -the quest for functional micrototal analysis systems epitomizes this. Microfluidic devices fabricated in glass, and more recently, in a variety of polymers, brings us a step closer to being able to achieve these stringent goals and to realize the economical fabrication of sophisticated instrumentation. However, this places a significant burden on the detection systems associated with microchip-based analysis systems. There is a need for a universal detector that can efficiently detect sample analytes in real time and with minimal sample manipulation steps, such as lengthy labeling protocols. This review highlights the advances in uncommon or less frequently used detection methods associated with microfluidic devices. As a result, the three most common methods -LIF, electrochemical, and mass spectrometric techniques -are omitted in order to focus on the more esoteric detection methods reported in the literature over the last 2 years.

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Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

Cited by 71 publications

References 191 publications

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Unconventional detection methods for microfluidic devices

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