Molecular genetic analysis of human cystic hydatid cases from Poland:
 identification of a new genotypic group (G9) of <i>Echinococcus granulosus</i>

Scott, Julie C.; Stefaniak, Jerzy; Pawłowski, Z.; McManus, Donald P.

doi:10.1017/s0031182096008062

Cited by 142 publications

(73 citation statements)

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“…The presence of E. granulosus genotypes G2, G5, G6 and G9 in humans was confirmed in Argentina and Poland (Guarnera et al, 2004;Rosenzvit et al, 1999;Scott et al, 1997), while in China previous studies from different areas revealed that human infections were caused by the G1 genotype (McManus et al, 1994;Yang et al, 2005;Zhang et al, 1998). However, in Xinjiang Uygur autonomous region the camel strain (G6) was also recently shown to infect humans (Bart et al, 2006).…”

Section: Discussionmentioning

confidence: 90%

“…Substantial genetic diversity of E. granulosus has led to the description of 10 genotypes (G1-10) (Bowles et al, 1992(Bowles et al, , 1995Bowles and McManus, 1993;Lavikainen et al, 2003;Nakao et al, 2007;Scott et al, 1997), and the G1 genotype has been proved to be cosmopolitan in its distribution and the major cause of human infection (McManus, 2002;Thompson and McManus, 2001). In China, previous genotypic analysis indicated the pathogenicity of the G6 genotype (dog/camel) in humans in Xinjiang autonomous region (Bart et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Species identification of human echinococcosis using histopathology and genotyping in northwestern China

Itō

Nakaya

et al. 2008

Transactions of the Royal Society of Tropical Medicine and Hygi

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SummaryHuman cystic echinococcosis, caused by infection with the larval stage of Echinococcus granulosus, and alveolar echinococcosis, caused by the larval form of E. multilocularis, are known to be important public health problems in western China. Echinococcus shiquicus is a new species of Echinococcus recently described in wildlife hosts from the eastern Tibetan plateau and its infectivity and/or pathogenicity in humans remain unknown. In the current study, parasite tissues from various organs were collected post-operatively from 68 echinococcosis patients from Sichuan and Qinghai provinces in eastern China. The tissues were examined by histopathology and genotyped using DNA sequencing and PCR-RFLP. Histopathologically, 38 human isolates were confirmed as E. granulosus and 30 as E. multilocularis. Mitochondrial cob gene sequencing and PCR-RFLP with rrnL as the target gene confirmed 33 of 53 of the isolates to have the G1 genotype of sheep/dog strain of E. granulosus as the only source of infection, while the remaining 20 isolates were identified as E. multilocularis. No infections were found to be caused by E. shiquicus. Additionally, 5 of 20 alveolar echinococcosis patients were confirmed to have intracranial metastases from primary hepatic alveolar echinococcosis lesions. All these cases originated from four provinces or autonomous regions but most were distributed in Sichuan and Qinghai provinces, where high prevalence rates of human alveolar echinococcosis and cystic echinococcosis were previously documented. * Corresponding author. Present address: 6 Zhong Xue Lu, Chengdu 610041, Sichuan Province, People's Republic of China. Tel.: +86 28 85589532; fax: +86 28 85589563. E-mail address: litiaoying@sina.com (T. Li). Authors' contributions: TL, AI, PSC and JQ designed the study protocol and drafted the manuscript; TL, PSC, AI and PG performed the analysis and interpretation of data; TL, MN, NX and XW conducted genetic analysis of all specimens; RZ did surgery; KN and MN did histopathology for morphological and molecular analysis; TL, CX and JQ collected tissue samples in the endemic areas. All authors read and approved the final manuscript. TL is guarantor of the paper.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Species identification of human echinococcosis using histopathology and genotyping in northwestern China

Itō

Nakaya

et al. 2008

Transactions of the Royal Society of Tropical Medicine and Hygi

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“…Characteristic PCR-amplified ITS1 and PCR-ITS1 RFLP banding patterns were produced when samples within Echinococcus species and strain group were analysed. The approach proved rapid and, although its usefulness has been questioned (Kedra et al 1999), it has proved applicable and reliable in the hands of a number of researchers for the identification of newly collected isolates and for the investigation of E. granulosus transmission patterns where strains occur sympatrically Wachira et al 1993 ;Bowles, Blair & McManus, 1994 ;Scott et al 1997 ;Rosenzvit et al 1999 ;Snabel et al 2000 ;Gonzalez et al 2002).…”

Section: Rflp/rapd Analysismentioning

confidence: 99%

“…Nuclear ribosomal ITS1-PCR-RFLP patterns and nad1 sequences were compared for human isolates of Polish origin (Scott et al 1997) collected by fine needle aspiration biopsy (FNAB). This is a procedure that, along with ultrasonography, allows differential diagnosis of suspected hepatic cysts in the liver and permits collection of parasite material for analysis from patients residing in areas where hydatid disease is relatively uncommon (Stefaniak & Lemke, 1995).…”

Section: Molecular Genetic Analysis Of Human Cystic Echinococcosis Camentioning

confidence: 99%

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Molecular epidemiology of cystic echinococcosis

2003

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Echinococcus granulosus exhibits substantial genetic diversity that has important implications for the design and development of vaccines, diagnostic reagents and drugs effective against this parasite. DNA approaches that have been used for accurate identification of these genetic variants are presented here as is a description of their application in molecular epidemiological surveys of cystic echinococcosis in different geographical settings and host assemblages. The recent publication of the complete sequences of the mitochondrial (mt) genomes of the horse and sheep strains of E. granulosus and of E. multilocularis, and the availability of mt DNA sequences for a number of other E. granulosus genotypes, has provided additional genetic information that can be used for more in depth strain characterization and taxonomic studies of these parasites. This very rich sequence information has provided a solid molecular basis, along with a range of different biological, epidemiological, biochemical and other molecular-genetic criteria, for revising the taxonomy of the genus Echinococcus. This has been a controversial issue for some time. Furthermore, the accumulating genetic data may allow insight to several other unresolved questions such as confirming the occurrence and precise nature of the E. granulosus G9 genotype and its reservoir in Poland, whether it is present elsewhere, why the camel strain (G6 genotype) appears to affect humans in certain geographical areas but not others, more precise delineation of the host and geographic ranges of the genotypes characterised to date, and whether additional genotypes of E. granulosus remain to be identified.

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Mutation scanning analysis of genetic variation within and among Echinococcus species: Implications and future prospects

Jabbar

Gasser

2013

Electrophoresis

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Adult tapeworms of the genus Echinococcus (family Taeniidae) occur in the small intestines of carnivorous definitive hosts and are transmitted to particular intermediate mammalian hosts, in which they develop as fluid-filled larvae (cysts) in internal organs (usually lung and liver), causing the disease echinococcosis. Echinococcus species are of major medical importance and also cause losses to the meat and livestock industries, mainly due to the condemnation of infected offal. Decisions regarding the treatment and control of echinococcosis rely on the accurate identification of species and population variants (strains). Conventional, phenetic methods for specific identification have some significant limitations. Despite advances in the development of molecular tools, there has been limited application of mutation scanning methods to species of Echinococcus. Here, we briefly review key genetic markers used for the identification of Echinococcus species and techniques for the analysis of genetic variation within and among populations, and the diagnosis of echinococcosis. We also discuss the benefits of utilizing mutation scanning approaches to elucidate the population genetics and epidemiology of Echinococcus species. These benefits are likely to become more evident following the complete characterization of the genomes of E. granulosus and E. multilocularis.

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Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus

Cited by 142 publications

References 7 publications

Species identification of human echinococcosis using histopathology and genotyping in northwestern China

Species identification of human echinococcosis using histopathology and genotyping in northwestern China

Molecular epidemiology of cystic echinococcosis

Mutation scanning analysis of genetic variation within and among Echinococcus species: Implications and future prospects

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