Molecular genetic characterization of Berari breed of goat using microsatellite markers

Kharkar, Kranti; Kuralkar, S. V.; Kuralkar, Prajakta

doi:10.5958/0976-0555.2015.00136.3

Cited by 10 publications

(8 citation statements)

References 10 publications

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“…The Katjang goat was found to generate a mean number of observed heterozygosity (0.29 ± 0.14), which was lower than the effective heterozygosity (0.72 ± 0.14). The observed heterozygosity was found to be lower than previously reported in 2013 for the Katjang goat breed and also lower than other studies in goat genetic diversity [ 43 , 45 , 54 , 59 , 61 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 ]. This showed that the genetic diversity of Katjang goats is decreasing and contains many loci with homozygous alleles as compared to other goat breeds.…”

Section: Discussioncontrasting

confidence: 77%

“…INRABERN172 and ILSTS005 both generated nine alleles and six alleles, as studied in the indigenous Gaddi goat breed from the Western Himalayas [ 59 ], while MAF209 produced nine alleles for the indigenous goats of Sub-Saharan Africa [ 60 ]. Another locus, RM004, generated 10 alleles in Asian goats, as studied by Barker et al [ 6 ], and also produced nine alleles, as studied in the Berari goat breed from India [ 61 ]. The monomorphic microsatellite loci obtained from this study could be due to alleles that had become fixed in the Katjang goat, indicating no genetic variation at these loci.…”

Section: Discussionmentioning

confidence: 99%

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Microsatellite-Based Genetic Characterization of the Indigenous Katjang Goat in Peninsular Malaysia

Muneerah

Tamrin

Salisi

et al. 2021

Animals

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The Katjang goat is the only indigenous domestic goat breed in Malaysia. Following a national baseline survey from 2001 to 2002, this breed was reported to the FAO as being at risk of extinction. In this study, 36 microsatellite markers were screened, and 25 polymorphic markers were used to analyze the genetic structure of the Katjang goat breed in Peninsular Malaysia. A sample set of data derived from another 10 populations from three published research studies was used as an outgroup for an inter-population genetic study. The analysis showed that the mean value of the observed heterozygosity was 0.29 ± 0.14, and the expected heterozygosity was 0.72 ± 0.14, which indicated low genetic diversity. The inbreeding coefficient, FIS, was high, at 0.46. Significant (p < 0.01) deviations from the Hardy Weinberg equilibrium were noted for all loci. The bottleneck analysis using the Wilcoxon Rank test under the two-phase model of mutation was significant (p < 0.01) for heterozygosity excess, which suggested that the Katjang breed had undergone significant population reduction in the past. Through combined analysis of data from publicly available research, almost the entire population of Katjang goats represent the centroid and are grouped together on a multidimensional scaling plot, except for the Terengganu population. Network analysis revealed that the goat population from Pahang formed the centrality of the network.

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Section: Discussioncontrasting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Microsatellite-Based Genetic Characterization of the Indigenous Katjang Goat in Peninsular Malaysia

Muneerah

Tamrin

Salisi

et al. 2021

Animals

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“…Mean observed heterozygosity (0.57) in Balangir goat was less than that in several recognized goat breeds of India, viz. Changthangi (0.60; Mishra et al 2010) Kharkar et al 2015), Chegu (0.80) and Gaddi (0.748) goat of Himachal Pradesh (Singh et al 2015). Observed heterozygosity was lower than expected showing a departure from Hardy-Weinberg Equilibrium (HWE) and the possibility of inbreeding.…”

Section: Resultsmentioning

confidence: 94%

Microsatellite markers based genetic diversity and differentiation of Balangir goat population of Odisha

Sharma

Ahlawat

Khadse

et al. 2021

Indian J of Anim Sci

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This study was executed to investigate the genetic diversity of Balangir, a local goat population from Odisha, and its differentiation from the geographically closest registered goat breeds, Ganjam and Black Bengal by utilizing 22 polymorphic microsatellite markers. The genotypic status of individuals at each locus was identified by an automated DNA sequencer and allelic data was analyzed for genetic diversity parameters. It resulted in the acquisition of original and comparable information confirming the genetic distinctness of the Balangir goat population. The observed number of alleles varied between 4 (RM4) and 16 (OMHC1) with 8.23Â±0.64 alleles per locus in Balangir goats. A moderate level of observed heterozygosity (0.57Â±0.05) indicated sufficiently existing genetic diversity in this goat population which varied between 0.09 (OarJMP29) and 1 (ILSTS22). Expected heterozygosity (0.64Â±0.04) oscillated between 0.18 (ILSTS34) and 0.89 (OMHC1). Within the population, heterozygosity deficiency estimate (FIS) was significantly positive (0.13Â±0.05) and 73% of the investigated loci contributed towards the homozygote excess. Consequently, significant deviation from Hardyâ€“Weinberg equilibrium was observed at 12 of 22 analyzed loci. Assessment of Balangir goat population for mutation drift equilibrium indicated that the population did not suffer a reduction in effective population size in the last few generations. Four different approaches utilized to study genetic relationships (F statistics, analysis of molecular variance, phylogenetic relationship genetic distance, and individual assignment) indicated that the Balangir goat population was genetically differentiated from the two registered goat breeds. The study highlighted that Balangir is discrete Indian goat germplasm with sufficient genetic variability and needs to be registered as a goat breed of India.

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“…Mean observed heterozygosity values similar to that of Palamu goat were observed in Black Bengal (0.69; Vijh et al 2010), Nandidurga (0.60;Tantia et al 2018) and Mahboobnagar (0.69; Raghavendra et al 2017) goats. On the other hand, higher values were noticed in Osmanabadi (0.71; Bhat et al 2013), Sanagamneri (0.73;Nath et al 2014), Berari (0.79;Kharkar et al 2015) and Chegu (0.80) and Gaddi (0.75) goat of Himachal Pradesh (Singh et al 2015). Much lower values have been observed in the goat populations of North Eastern Hill (NEH) region such as Assam hill goat (0.48; Zaman et al 2013) and Sumi-Ne goat of Nagaland (0.49; Verma et al 2019).…”

Section: Resultsmentioning

confidence: 95%

Characterization of a new potential goat breed (Palamu) from Jharkhand, India

Sharma

Vij

Aggarwal

et al. 2021

Indian J of Anim Sci

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Palamu goat forms the part and parcel of the lives of the farmers and tribes of Jharkhand. Palamu goat also known as Medini is named after its distribution area (Palamu, Latehar and Garhwa) in Jharkhand. This small size goat is reared in Jharkhand since time immemorial. Average flock size is 5.36±0.19 and varies from 1 to 31. Twinning is common except in first kidding. Head profile is convex, ears are pendulous, and horns are straight with backward and upward orientation. Muzzle, eyelids and hooves are black, body is cylindrical, udder is small and pendulous and teats are conical shaped. Age at first mating in males is 8.32±0.86 (months) that vary between 7.2– 9.2 months. Corresponding values for female are 7.22±1.35 months varying between 6.7–7.7 months. Diversity status of the population was explored using 25 microsatellite markers. A total of 190 alleles were detected and sufficient polymorphism was evident from the allele frequency data. ILSTS82 showed the highest number of observed alleles per locus (20) while RM4 and ILSTS05 showed the lowest (4) with 9.14±2.0 as mean number of alleles. Expected number of alleles varied from 1.49 (ILSTS065) to 7.55 (ILSTS30) with the mean value of 4.15±0.91. Palamu goat had substantial genetic variation based on its gene diversity in addition to the average number of alleles per locus. The observed and expected heterozygosity values were 0.64±0.14 and 0.69±0.15, respectively. Observed heterozygosity was lower than expected showing a departure from Hardy-Weinberg Equilibrium (HWE) and possibility of inbreeding. Population has heterozygote deficiency to the tune of 9% (FIS value=0.09). Population did not suffer from recent genetic bottleneck (last 40–80 generations). The results suggest existence of a distinct goat population harboring sufficient genetic variation for scientific management.

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Molecular genetic characterization of Berari breed of goat using microsatellite markers

Cited by 10 publications

References 10 publications

Microsatellite-Based Genetic Characterization of the Indigenous Katjang Goat in Peninsular Malaysia

Microsatellite-Based Genetic Characterization of the Indigenous Katjang Goat in Peninsular Malaysia

Microsatellite markers based genetic diversity and differentiation of Balangir goat population of Odisha

Characterization of a new potential goat breed (Palamu) from Jharkhand, India

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