Molecular genetic diagnosis of de novo and recurrent bladder cancer

Steiner, Gabriel; Reinschmidt, Gudrun; Müller, Stefan

doi:10.1002/(sici)1522-2683(19990201)20:2<280::aid-elps280>3.0.co;2-z

Cited by 13 publications

(15 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[15][16][17] Microsatellite instability is also a distinctive feature in nearly 15-20% of sporadic colorectal tumors. [18][19][20] Replication-error-positive phenotype has been detected recently in other solid tumors [21][22][23][24] and in various lymphomas and leukemias. [25][26][27][28][29][30] The involvement of microsatellite instability in the pathogenesis of gastric lymphoma of MALT type is controversial, as both negative and positive findings were reported.…”

mentioning

confidence: 99%

Microsatellite instability in gastric MALT lymphoma

et al. 2004

View full text Add to dashboard Cite

The role of microsatellite instability and defects in DNA mismatch repair mechanism in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) type is still controversial, as both negative and positive findings have been reported. This may be explained mainly by arbitrary selection of the tested loci, the use of various techniques of microsatellite instability analysis and by different definitions of replication error positive phenotype. The aim of our study was to evaluate the instability at selected microsatellite markers using the GeneScan Analysis Software. DNA from paraffin-embedded tissue blocks of 13 previously untreated patients with localized gastric MALT lymphoma was extracted. Five microsatellite markers, which are located in hMSH2, hMLH1, P16, APC and MLL loci, were selected from the genetic database. We found genetic instability in tumors of 9/13 patients with gastric MALT lymphoma (69%). Seven of them had replication-error-positive phenotype (54%). Microsatellite instability was found in 39% of the samples in the MLL locus, 39% in the APC, 46% in the P16, 23% in the hMLH1 and none in the hMSH2. This study demonstrates that microsatellite instability has more prominent role in pathogenesis of gastric MALT lymphoma than reported to date. We suggest that microsatellite instability should be analyzed with markers adjacent to chromosomal loci that are involved in lymphomas. Our findings support the 'Real Common Target genes' theory of high rate of microsatellite instability in specific genes, which are associated with related tumors.

show abstract

mentioning

confidence: 99%

Microsatellite instability in gastric MALT lymphoma

et al. 2004

View full text Add to dashboard Cite

show abstract

“…For this reason, molecular approaches are being contemplated. PCR-based studies have been carried out that claim up to 91% diagnostic sensitivity [13]. In this pilot study, MSI positive cases have shown a good correlation with superficial high grade tumors, which may help in deciding upon radical surgery to begin with.…”

Section: Discussionmentioning

confidence: 96%

“…Since genetic alterations precede histological manifestation, its documentation could become a rational approach for early detection of various malignancies. Fine Needle Aspiration Biopsy (FNAB) for thyroid cancer and cystoscopic evaluation for bladder cancer are gold standards for diagnosis and follow-up for recurrences [12,13]. However these measures are invasive and may cause discomfort and infection to the patients and therefore their routine application in screening cannot always be advocated [13].…”

Section: Introductionmentioning

confidence: 99%

“…Fine Needle Aspiration Biopsy (FNAB) for thyroid cancer and cystoscopic evaluation for bladder cancer are gold standards for diagnosis and follow-up for recurrences [12,13]. However these measures are invasive and may cause discomfort and infection to the patients and therefore their routine application in screening cannot always be advocated [13]. Moreover, early diagnosis for these recurrent cancers is critical for successful treatment, thereby increasing the need for developing more sensitive and cost-effective diagnostic tools.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of microsatellite instability in bladder and thyroid malignancies

Vaish

Mishra

Mandhani

et al. 2003

Teratog. Carcinog. Mutagen.

View full text Add to dashboard Cite

Microsatellite instability (MSI) is an indicator of a defective DNA mismatch repair system (MMR) that results from somatic mutations. The present work has been planned to investigate MSI and its clinical significance in human urinary bladder and thyroid cancers in Indian patients. Tumor tissues of histologically confirmed cases of urinary bladder and thyroid cancers, respectively, were obtained. Clinical data on tumor stage and histopathological grades were recorded. Corresponding matched peripheral blood was taken as a control. Genomic DNA was isolated from the tumor tissues and blood using a standard phenol-chloroform extraction method. Polymerase chain reaction was done to amplify mononucleotide microsatellite markers, BAT-26, BAT-40, TGFbetaRII, IGFIIR, hMSH3, and Bax by using specific primer sequences. For analysis of allelic patterns, the PCR products were run on 8% denaturing Polyacrylamide gel and sizing was done using a pUC18 sequencing ladder. The instability with BAT-26 and BAT-40 was found to be 20% and 45% in urinary bladder and 33% and 19% in thyroid cancers, respectively. However, no instability was observed with the other four-mononucleotide markers in either of the cancers studied. Eighty-three percent of the unstable urinary bladder cancers were found to have a high grade in a superficial group, whereas only 27% MSI+ve were muscle invasive cancers. Forty percent of unstable thyroid lesions were found to be at high risk of developing metastasis. Association of BAT-26 and BAT-40 instabilities with high grade tumors as well as risk tumors may help in choosing a more definite therapy at the outset.

show abstract

“…Die Spezifität erreicht dabei, bestätigt durch andere Arbeitsgruppen, 100%. In weiteren Untersuchungen konnte gezeigt werden,dass diese Technologie eindeutig der konventionellen Urinzytologie überlegen ist und Rezidive frühzeitiger als mittels des "golden standard" der Zystoskopie vorhergesagt werden können [34,35].…”

Section: Mikrosatellitenanalyse Beim Harnblasenkarzinomunclassified

Molekulare Diagnostik in der urologischen Onkologie

et al. 2003

View full text Add to dashboard Cite

The goal of molecular diagnostics in oncology is the early diagnosis of malignant disease processes during initial work-up or as part of follow-up. Body fluids serve as the primary material for non-invasive diagnostic methods. Besides actual tumor cells, the examination of urine can yield evidence of secreted proteins or even free nucleic acids. In principle, all of the methods available for the detection of tumor markers in tissue or blood samples can be successfully applied to the examination of urine samples. However, molecular biological examination of urine samples is associated with important problems because the cells in such samples are exposed to significant degradation and regression effects and because certain components of the urine act to inhibit the polymerase chain reaction. The present overview discusses the respective strengths and weakness of the available technology as applied to the diagnosis of urologic malignancies. Experimental studies conducted to date have reported high sensitivities and specificities for molecular diagnostics using urine samples. It is important to note that not only carcinomas of the urinary bladder can be diagnosed from material obtained in urine samples: in fact, the method can be used to diagnose entities such as renal cell and prostate carcinomas and, due to renal filtration of DNA, even non-urologic malignancies. The diagnostic application of these methods, however, remains in an experimental stage and must still clear several hurdles before becoming available for routine clinical use.

show abstract

Molecular genetic diagnosis of de novo and recurrent bladder cancer

Cited by 13 publications

References 17 publications

Microsatellite instability in gastric MALT lymphoma

Microsatellite instability in gastric MALT lymphoma

Assessment of microsatellite instability in bladder and thyroid malignancies

Molekulare Diagnostik in der urologischen Onkologie

Contact Info

Product

Resources

About