Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Johnson, Tylor J.; Gibbons, Jaimie; Gu, Liping; Zhou, Ruanbao; Gibbons, William R.

doi:10.1002/btpr.2358

Cited by 33 publications

(19 citation statements)

References 212 publications

(451 reference statements)

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“…(Johnson et al . ). Currently, very few studies reported application of a CRISPR/Cas9 genome editing system in the fast‐growing cyanobacterium S. elongatus UTEX 2973, as a proof of concept for the ability to produce a marker‐free deletion mutant to target the nblA gene (Wendt et al .…”

Section: Genome Editing Toolsmentioning

confidence: 97%

Advances and challenges in genetic engineering of microalgae

Fajardo

Donato

Carrasco

et al. 2019

Reviews in Aquaculture

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Knowledge at the molecular level of microalgae has obtained great scientific interest in recent years, because of their potential for biofuel production, as well as other highly valued molecules. Recent advances in the area of the genetic manipulation of microalgae open an entirely new field of possible applications for these organisms as biorefineries. This review represents a compendium that gathers the main milestones that have marked the area of genetic engineering in microalgae during the last decade, from classical techniques for the transformation and expression of transgenes, to the most avant‐garde techniques currently used for gene silencing and DNA edition, as well as a discussion about the future perspectives in this field.

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Section: Genome Editing Toolsmentioning

confidence: 97%

Advances and challenges in genetic engineering of microalgae

Fajardo

Donato

Carrasco

et al. 2019

Reviews in Aquaculture

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“…However, these systems are not feasible for the production of fourth generation biofuels as many of these chemicals are volatile, thus cannot be maintained in an open system. Fourth generation biofuels are derived from genetically modified POs that directly convert CO 2 into biofuel chemicals . Another disadvantage of pond systems is the use of genetically modified POs in these systems is typically not allowed by policymakers due to the risk of these strains contaminating the surrounding environment.…”

Section: Large‐scale Cultivation Systems For Microalgae and Cyanobactmentioning

confidence: 99%

Photobioreactor cultivation strategies for microalgae and cyanobacteria

Johnson

Katuwal

Anderson

et al. 2018

Biotechnology Progress

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The current burden on fossil-derived chemicals and fuels combined with the rapidly increasing global population has led to a crucial need to develop renewable and sustainable sources of chemicals and biofuels. Photoautotrophic microorganisms, including cyanobacteria and microalgae, have garnered a great deal of attention for their capability to produce these chemicals from carbon dioxide, mineralized water, and solar energy. While there have been substantial amounts of research directed at scaling-up production from these microorganisms, several factors have proven difficult to overcome, including high costs associated with cultivation, photobioreactor construction, and artificial lighting. Decreasing these costs will substantially increase the economic feasibility of these production processes. Thus, the purpose of this review is to describe various photobioreactor designs, and then provide an overview on lighting systems, mixing, gas transfer, and the hydrodynamics of bubbles. These factors must be considered when the goal of a production process is economic feasibility. Targets for improving microalgae and cyanobacteria cultivation media, including water reduction strategies will also be described. As fossil fuel reserves continue to be depleted and the world population continues to increase, it is imperative that renewable chemical and biofuel production processes be developed toward becoming economically feasible. Thus, it is essential that future research is directed toward improving these processes. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:811-827, 2018.

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“…Diversity of hydrocarbons produced by Cyanobacteria in terms of chain length, degree of saturation, and variations of the carbon skeleton has been highlighted by Xie et al (2017). Considerable efforts have been directed to metabolic engineering of Cyanobacteria, mainly geared towards biofuel production (Al-Haj et al 2016; Oliver et al 2016; Johnson et al 2016; Gomaa et al 2016). The major optimization strategies were directed towards improving carbon fixation (Atsumi et al 2009), improving the capture of the light energy (Iwaki et al 2006), and improving product extraction by the use of induced autolysis systems (Miyake et al 2014).…”

Section: Cyanobacteria: the Potential To Derive Biomass From Solar Enmentioning

confidence: 99%

Building a bio-based industry in the Middle East through harnessing the potential of the Red Sea biodiversity

Nielsen

Archer

Essack

et al. 2017

Appl Microbiol Biotechnol

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The incentive for developing microbial cell factories for production of fuels and chemicals comes from the ability of microbes to deliver these valuable compounds at a reduced cost and with a smaller environmental impact compared to the analogous chemical synthesis. Another crucial advantage of microbes is their great biological diversity, which offers a much larger “catalog” of molecules than the one obtainable by chemical synthesis. Adaptation to different environments is one of the important drives behind microbial diversity. We argue that the Red Sea, which is a rather unique marine niche, represents a remarkable source of biodiversity that can be geared towards economical and sustainable bioproduction processes in the local area and can be competitive in the international bio-based economy. Recent bioprospecting studies, conducted by the King Abdullah University of Science and Technology, have established important leads on the Red Sea biological potential, with newly isolated strains of Bacilli and Cyanobacteria. We argue that these two groups of local organisms are currently most promising in terms of developing cell factories, due to their ability to operate in saline conditions, thus reducing the cost of desalination and sterilization. The ability of Cyanobacteria to perform photosynthesis can be fully exploited in this particular environment with one of the highest levels of irradiation on the planet. We highlight the importance of new experimental and in silico methodologies needed to overcome the hurdles of developing efficient cell factories from the Red Sea isolates.

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Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Cited by 33 publications

References 212 publications

Advances and challenges in genetic engineering of microalgae

Advances and challenges in genetic engineering of microalgae

Photobioreactor cultivation strategies for microalgae and cyanobacteria

Building a bio-based industry in the Middle East through harnessing the potential of the Red Sea biodiversity

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