Molecular genetic mechanisms of influence of laser radiation with 577 nm wavelength in a microimpulse mode on the condition of the retina

Gavrilova, N. A.; Борзенок, С.А.; Zaletaev, D. V.; Соломин, В А; Gadzhieva, N.S.; Tishchenko, O.E.; Komova, Olga U.; Зиновьева, А. В.

doi:10.1016/j.exer.2019.04.018

Cited by 3 publications

(2 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…После снижения при введении VEGF нормализовалась экспрессия гена TKT, кодирующего белок с транскетолазной активностью. Установ-лено, что у животных с стрептозотоцининдуцированным сахарным диабетом в результате активации TKT при использовании бенфотиамина (жирорастворимое производное тиамина) блокируются все пути образования метаболитов глюкозы, предотвращается развитие сосудистых патологических изменений и диабетической ретинопатии [14]. При проведении аргонлазерной коагуляции сетчатки мышей C75Bl/6J экспрессия гена TKT остается повышенной в течение длительного времени (90 дней); авторы считают, что этот ген может являться потенциальным геном-мишенью в отношении предотвращения развития диабетической ретинопатии и неоваскуляризации [1].…”

Section: результаты исследования и их обсуждениеunclassified

“…В предыдущем исследовании нами было изучено изменение ретинальной экспрессии генов после воздействия лазерного излучения с длиной волны 577 нм в микроимпульсном режиме на основе анализа транскрипции микрочипов в условиях VEGF-индуцированной патологии (Vascular Endothelial Growth Factor) [14]. Преимущества лазерного излучения с длиной волны 577 нм заключаются в его минимальном поглощении макулярным ксантофильным пигментом, что делает его применение относительно безопасным вблизи фовеа [30], а также в высоком поглощении оксигемоглобином и меланином, что вызывает меньшее рассеивание в сравнении с длиной волны 532 нм или другими желтыми волнами (561/568 нм) [36].…”

unclassified

See 1 more Smart Citation

Analysis of retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function following exposure of the retina to 577 nm wavelength laser light in continuous mode

Gavrilova¹,

Гуревич²,

Komova³

et al. 2021

Humans and their Health

View full text Add to dashboard Cite

Objective. The aim of the study was to investigate the retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function after exposure of the retina to 577 nm laser light in a continuous mode in experimental conditions after intravitreal injection of VEGF165 (Vascular endothelial growth factor). Materials and methods. The study was performed on 4-5 week old male C57BL / 6J mice. 4 groups, 5 mice in each, were formed, one animal eye was experimental and the second remained intact. The animals of the first group received intravitreal injection of phosphate-buffered saline (PBS); animals in the second, third and fourth groups received intravitreal injection of 50 ng/ml of recombinant VEGF165; in the third and fourth groups, one day after the VEGF165 intravitreal administration, laser irradiation with a wavelength of 577 nm was performed on the retina in micropulse and continuous modes, respectively. Tissue samples from animals of the first and second groups were taken 2 days after PBS and VEGF165 administration, and in animals from the third and fourth groups one day after laser exposure to the retina. Results. Retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function as a result of retinal exposure to 577 nm laser radiation in continuous mode following intravitreal injection of VEGF165 was studied. Genes with significant changes in expression levels were identified (genes that regulate the processes of angiogenesis). Conclusion. Understanding the mechanisms of modulation of retinal gene expression may help to identify the key regulatory factors providing therapeutic effects of laser radiation in continuous and micropulsed modes and provide the basis for future therapeutic tactics for retinal diseases.

show abstract

Section: результаты исследования и их обсуждениеunclassified

unclassified

Analysis of retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function following exposure of the retina to 577 nm wavelength laser light in continuous mode

Gavrilova¹,

Гуревич²,

Komova³

et al. 2021

Humans and their Health

View full text Add to dashboard Cite

show abstract

Regenerative Retinal Laser and Light Therapies (RELITE): Proposal of a New Nomenclature, Categorization, and Trial Reporting Standard

von der Burchard,

Miura,

Stanzel

et al. 2024

Lasers Surg Med

View full text Add to dashboard Cite

ObjectivesNumerous laser and light therapies have been developed to induce regenerative processes in the choroid/retinal pigment epithelium (RPE)/photoreceptor complex, leaving the neuroretina undamaged. These therapies are applied to the macula for the treatment of various diseases, most prominently diabetic maculopathy, retinal vein occlusion, central serous chorioretinopathy, and age‐related macular degeneration. However, the abundance of technologies, treatment patterns, and dosimetry protocols has made understanding these therapies and comparing different approaches increasingly complex and challenging. To address this, we propose a new nomenclature system with a clear categorization that will allow for better understanding and comparability between different laser and light modalities. We propose this nomenclature system as an open standard that may be adapted in future toward new technical developments or medical advancements.MethodsA systematic literature review of reported macular laser and light therapies was conducted. A categorization into a standardized system was proposed and discussed among experts and professionals in the field. This paper does not aim to assess, compare, or evaluate the efficacy of different laser or dosimetry techniques or treatment patterns.ResultsThe literature search yielded 194 papers describing laser techniques, 50 studies describing dosimetry, 272 studies with relevant clinical trials, and 82 reviews. Following the common therapeutic aim, we propose “regenerative retinal laser and light therapies (RELITE)” as the general header. We subdivided RELITE into four main categories that refer to the intended physical and biochemical effects of temperature increase (photothermal therapy, PTT), RPE regeneration (photomicrodisruption therapy, PMT), photochemical processes (photochemical therapy, PCT), and photobiomodulation (photobiomodulation therapy, PBT).Further, we categorized the different dosimetry approaches and treatment regimens. We propose the following nomenclature system that integrates the most important parameters to enable understanding and comparability:Pattern—Dosimetry—Exposure Time/Frequency, Duty Cycle/Irradiation Diameter/Wavelength—Subcategory—Category.ConclusionRegenerative retinal laser and light therapies are widely used for different diseases and may become valuable in the future. A precise nomenclature system and strict reporting standards are needed to allow for a better understanding, reproduceable and comparable clinical trials, and overall acceptance. We defined categories for a systematic therapeutic goal‐based nomenclature to facilitate future research in this field.

show abstract

Retinale Lasertherapie

Tode,

Volkmann,

Framme

2024

Springer Reference Medizin

View full text Add to dashboard Cite

Molecular genetic mechanisms of influence of laser radiation with 577 nm wavelength in a microimpulse mode on the condition of the retina

Cited by 3 publications

References 103 publications

Analysis of retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function following exposure of the retina to 577 nm wavelength laser light in continuous mode

Analysis of retinal expression of genes involved in the regulation of angiogenesis and endothelial barrier function following exposure of the retina to 577 nm wavelength laser light in continuous mode

Regenerative Retinal Laser and Light Therapies (RELITE): Proposal of a New Nomenclature, Categorization, and Trial Reporting Standard

Retinale Lasertherapie

Contact Info

Product

Resources

About