Molecular genetic study of introgression between <i>Saccharomyces bayanus</i> and <i>S. cerevisiae</i>

Наумова, Е. С.; Наумов, Г. И.; Masneuf‐Pomarède, Isabelle; Aigle, Michel; Dubourdieu, Denis

doi:10.1002/yea.1298

Cited by 121 publications

(115 citation statements)

References 65 publications

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“…For the two strains with dual species introgression, this implies that progenitors of these strains either (1) successively hybridized with each of the other two species, or (2) hybridized with one of the other species, which already contained the other introgression. In all cases, it appears that the introgressed regions occur near or within subtelomeric regions (Naumova et al 2005(Naumova et al , 2011, and are found among the various strains in either homozygous (lacking equivalent S. cerevisiae sequences) or hemizygous states.…”

Section: Interspecific Hybridization and Introgressionmentioning

confidence: 96%

“…Furthermore, introgression events, defined as a relatively small region of a different species' genome found within the genome of another species and mostly occurring within the closely related species of the Saccharomyces sensu stricto group, have also been detected (Naumova et al 2005;Liti et al 2006;Doniger et al 2008;Muller and McCusker 2009a,b;Naumova et al 2011). Horizontal gene transfer, in which genes are transferred from a distantly related organism (even transkingdom) through nonsexual mechanisms, has also been described among the Saccharomyces yeasts (Hall et al 2005;Novo et al 2009;Rolland et al 2009), although it may be relatively rare.…”

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confidence: 99%

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Analysis of the Saccharomyces cerevisiae pan-genome reveals a pool of copy number variants distributed in diverse yeast strains from differing industrial environments

Dunn¹,

Richter²,

Kvitek³

et al. 2012

Genome Res.

205

182

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Although the budding yeast Saccharomyces cerevisiae is arguably one of the most well-studied organisms on earth, the genome-wide variation within this species—i.e., its “pan-genome”—has been less explored. We created a multispecies microarray platform containing probes covering the genomes of several Saccharomyces species: S. cerevisiae, including regions not found in the standard laboratory S288c strain, as well as the mitochondrial and 2-μm circle genomes–plus S. paradoxus, S. mikatae, S. kudriavzevii, S. uvarum, S. kluyveri, and S. castellii. We performed array-Comparative Genomic Hybridization (aCGH) on 83 different S. cerevisiae strains collected across a wide range of habitats; of these, 69 were commercial wine strains, while the remaining 14 were from a diverse set of other industrial and natural environments. We observed interspecific hybridization events, introgression events, and pervasive copy number variation (CNV) in all but a few of the strains. These CNVs were distributed throughout the strains such that they did not produce any clear phylogeny, suggesting extensive mating in both industrial and wild strains. To validate our results and to determine whether apparently similar introgressions and CNVs were identical by descent or recurrent, we also performed whole-genome sequencing on nine of these strains. These data may help pinpoint genomic regions involved in adaptation to different industrial milieus, as well as shed light on the course of domestication of S. cerevisiae.

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Section: Interspecific Hybridization and Introgressionmentioning

confidence: 96%

mentioning

confidence: 99%

Analysis of the Saccharomyces cerevisiae pan-genome reveals a pool of copy number variants distributed in diverse yeast strains from differing industrial environments

Dunn¹,

Richter²,

Kvitek³

et al. 2012

Genome Res.

205

182

View full text Add to dashboard Cite

show abstract

“…This phenomenon was mentioned a few years ago for Saccharomyces, based on classical molecular genetic methods (32,148), and was recently extended using microsatellite typing (149). With complete genome sequences, introgression of delineated chromosomal fragments from one yeast species to another appears more frequent than initially anticipated.…”

Section: Traces Of Natural Introgressions In Yeast Genomesmentioning

confidence: 97%

Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Morales

Dujon

2012

Microbiol Mol Biol Rev

155

158

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SUMMARYForced interspecific hybridization has been used in yeasts for many years to study speciation or to construct artificial strains with novel fermentative and metabolic properties. Recent genome analyses indicate that natural hybrids are also generated spontaneously between yeasts belonging to distinct species, creating lineages with novel phenotypes, varied genetic stability, or altered virulence in the case of pathogens. Large segmental introgressions from evolutionarily distant species are also visible in some yeast genomes, suggesting that interspecific genetic exchanges occur during evolution. The origin of this phenomenon remains unclear, but it is likely based on weak prezygotic barriers, limited Dobzhansky-Muller (DM) incompatibilities, and rapid clonal expansions. Newly formed interspecies hybrids suffer rapid changes in the genetic contribution of each parent, including chromosome loss or aneuploidy, translocations, and loss of heterozygosity, that, except in a few recently studied cases, remain to be characterized more precisely at the genomic level by use of modern technologies. We review here known cases of natural or artificially formed interspecies hybrids between yeasts and discuss their potential importance in terms of genome evolution. Problems of meiotic fertility, ploidy constraint, gene and gene product compatibility, and nucleomitochondrial interactions are discussed and placed in the context of other known mechanisms of yeast genome evolution as a model for eukaryotes.

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“…The fact that this organism is an interspecific hybrid has been known for more than two decades (e.g., Nilsson-Tillgren et al 1981;Martini and Martini 1987; for reviews, see Kodama et al 2005;Smart 2007), and work since then has shown conclusively that different brewing yeast isolates can contain different combinations of the genomes (or partial genomes) of the following closely related Saccharomyces sensu stricto species: S. cerevisiae, Saccharomyces bayanus, Saccharomyces bayanus var. uvarum, and possibly a fourth, unknown "lagertype" species (Casaregola et al 2001;Naumova et al 2005;Rainieri et al 2006; for reviews, see Hornsey 2003;Kodama et al 2005). Pulsed-field gel/Southern blot analysis of several S. pastorianus strains has shown distinct differences in the distribution of transposable elements (Pederson 1985;Liti et al 2005), while array-CGH studies, using S. cerevisiae-only arrays, showed limited chromosomal changes among several strains (Bond et al 2004;Kodama et al 2005), also indicating complexity within this group.…”

mentioning

confidence: 99%

Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianus

Dunn¹,

Sherlock²

2008

Genome Res.

259

391

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Inter-specific hybridization leading to abrupt speciation is a well-known, common mechanism in angiosperm evolution; only recently, however, have similar hybridization and speciation mechanisms been documented to occur frequently among the closely related group of sensu stricto Saccharomyces yeasts. The economically important lager beer yeast Saccharomyces pastorianus is such a hybrid, formed by the union of Saccharomyces cerevisiae and Saccharomyces bayanus-related yeasts; efforts to understand its complex genome, searching for both biological and brewing-related insights, have been underway since its hybrid nature was first discovered. It had been generally thought that a single hybridization event resulted in a unique S. pastorianus species, but it has been recently postulated that there have been two or more hybridization events. Here, we show that there may have been two independent origins of S. pastorianus strains, and that each independent group—defined by characteristic genome rearrangements, copy number variations, ploidy differences, and DNA sequence polymorphisms—is correlated with specific breweries and/or geographic locations. Finally, by reconstructing common ancestral genomes via array-CGH data analysis and by comparing representative DNA sequences of the S. pastorianus strains with those of many different S. cerevisiae isolates, we have determined that the most likely S. cerevisiae ancestral parent for each of the independent S. pastorianus groups was an ale yeast, with different, but closely related ale strains contributing to each group’s parentage.

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Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae

Cited by 121 publications

References 65 publications

Analysis of the Saccharomyces cerevisiae pan-genome reveals a pool of copy number variants distributed in diverse yeast strains from differing industrial environments

Analysis of the Saccharomyces cerevisiae pan-genome reveals a pool of copy number variants distributed in diverse yeast strains from differing industrial environments

Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in Yeasts

Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianus

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