Molecular Genetic Typing of Yersinia enterocolitica Serovar O:8 Isolated in Japan

Hayashidani, Hideki; Ishiyama, Yuki; Okatani, Tomomitsu A.; Yoshida, Shinichiro; Ishikawa, Masaaki; Kato, Yukio; Ohtomo, Yoshimitsu; Saito, Masaaki; Horisaka, Tomoko; Kaneko, Ken‐ichi; Ogawa, Masahiro

doi:10.1007/0-306-48416-1_72

Cited by 13 publications

(20 citation statements)

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“…A comparison of the molecular genotypes of the 30 isolates from our study with other Japanese isolates analyzed by Hayashidani et al 12 showed that the molecular genotype of 29 isolates was very similar to that of the strains isolated from wild rodents captured in Niigata Prefecture, which borders the Kanto region in the northwest. It also showed that the genotype of the one isolate that differed from the other 29 showed a molecular genotype similar to that of an isolate from a wild rodent in Yamagata Prefecture in northeastern Japan (Table 4).…”

Section: Characteristics Of Pathogenic Yersinia Isolated From Breedinsupporting

confidence: 71%

“…Hayashidani et al 12 have classified Japanese O:8 isolates into seven genotypes based on a combination of the results of PFGE and ribotyping. A comparison of the molecular genotypes of the 30 isolates from our study with other Japanese isolates analyzed by Hayashidani et al 12 showed that the molecular genotype of 29 isolates was very similar to that of the strains isolated from wild rodents captured in Niigata Prefecture, which borders the Kanto region in the northwest.…”

Section: Characteristics Of Pathogenic Yersinia Isolated From Breedinmentioning

confidence: 99%

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Epidemiological Findings on Yersiniosis in Nonhuman Primates in Zoological Gardens in Japan

Iwata

Hayashidani

2011

JARQ

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Yersiniosis, which is caused by pathogenic Yersinia enterocolitica or Yersinia pseudotuberculosis, poses a serious problem for zoological gardens engaged in breeding nonhuman primates. In Japan, Y. pseudotuberculosis in particular frequently causes fatal infection, and affected nonhuman primates may die unexpectedly or after a very short illness. Our epidemiological study in 17 zoological gardens in Japan suggested that Yersinia pseudotuberculosis-derived mitogen (YPM), which is a kind of superantigenic toxin, might be the cause of, or at least the most important factor in, the high mortality of breeding nonhuman primates infected by Y. pseudotuberculosis in Japan. Furthermore, seroepidemiological study proved that pathogenic Yersinia is highly prevalent among breeding squirrel monkeys in Japan. It is likely that the monkeys that are pathogenic Yersinia positive have been inapparently or mildly infected by low pathogenic strains of Yersinia, not highly pathogenic strains of Yersinia, such as YPM-producing Y. pseudotuberculosis. In this review, we will describe the epidemiology of yersiniosis in breeding nonhuman primates in Japan.

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Section: Characteristics Of Pathogenic Yersinia Isolated From Breedinsupporting

confidence: 71%

Section: Characteristics Of Pathogenic Yersinia Isolated From Breedinmentioning

confidence: 99%

Epidemiological Findings on Yersiniosis in Nonhuman Primates in Zoological Gardens in Japan

Iwata

Hayashidani

2011

JARQ

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“…Y. enterocolitica were isolated from wild animals [52, 53] for example, from 16 of 495 small wild animals (mainly mice) and from 1 of 38 foxes [52], the isolated serotype were O:6, O:5A, O:4, and O:9. Wild rodents and pigs have been shown to be reservoirs for Y. enterocolitica O:8 strains in Japan [9]. Strains of very rare bioserotypes, such as bioserotype 5/O:2, 3, have been isolated from sheep, hares, and goats and bioserotype 3/O:1, 2a, 3 from chinchillas.…”

Section: Reservoirsmentioning

confidence: 99%

“…enterocolitica because pig is so far the only animal species from which pathogenic strains have frequently been isolated [8]. Several domestic animals like dogs, cats, cows, sheep, and horses and several wild [9] animals like rodents (mainly mice), monkeys, deer, and foxes have also been incriminated as potential reservoirs [10]. …”

Section: Introductionmentioning

confidence: 99%

Behavior ofYersinia enterocoliticain Foods

Bari

Hossain

Isshiki

et al. 2011

Journal of Pathogens

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Yersinia enterocolitica are ubiquitous, being isolated frequently from soil, water, animals, and a variety of foods. They comprise a biochemically heterogeneous group that can survive and grow at refrigeration temperatures. The ability to propagate at refrigeration temperatures is of considerable significance in food hygiene. Virulent strains of Yersinia invade mammalian cells such as HeLa cells in tissue culture. Two chromosomal genes, inv and ail, were identified for cell invasion of mammalian. The pathogen can cause diarrhoea, appendicitis and post-infection arthritis may occur in a small proportion of cases. The most common transmission route of pathogenic Y. enterocolitica is thought to be fecal-oral via contaminated food. Direct person-to-person contact is rare. Occasionally, pathogenic Y. enterocolitica has been detected in vegetables and environmental water; thus, vegetables and untreated water are also potential sources of human yersiniosis. However, the isolation rates of pathogenic Y. enterocolitica have been low, which may be due to the limited sensitivity of the detection methods. To identify other possible transmission vehicles, different food items should be studied more extensively. Many factors related to the epidemiology of Y. enterocolitica, such as sources, transmission routes, and predominating genotypes remain obscure because of the low sensitivity of detection methods.

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“…The main source of O:3 and O:5,27 infection is meat. On the other hand, infection with Y. enterocolitica O:8 results from water contaminated with the micro-organism [9]. It is suspected that soil and water become contaminated with these bacteria via wild rodents, which are an important natural reservoir of these pathogenic serovars [8].…”

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Immuno-Magnetic Separation and Agar Layer Methods for the Isolation of Freeze-Injured Yersinia enterocolitica O:8 from Water

Koujitani

Horisaka

Nomura

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. To develop an effective method to isolate an injured pathogenic Yersinia enterocolitica O:8 organism from environmental samples, we compared the isolation of freeze-injured and non-injured Y. enterocolitica O:8 and found that the isolation was more successful when immuno-magnetic separation (IMS) with anti-Y. enterocolitica O:8 antibody was used. Plating onto cefsulodin-irgasan-novobiocin (CIN) agar and Virulent Yersinia enterocolitica (VYE) agar by means of the agar layer method was found to be effective in isolating the injured cells. The alkali treatment which is generally used for selective detection of Yersinia organism failed to isolate freeze-injured pathogenic Y. enterocolitica O:8 cells. Recovery methods without using the alkali treatment were superior for detecting freeze-injured Y. enterocolitica O:8. Our results demonstrate that the IMS and the agar layer methods should be used to isolate injured pathogenic Yersinia organisms from environmental samples such as water.

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Molecular Genetic Typing of Yersinia enterocolitica Serovar O:8 Isolated in Japan

Cited by 13 publications

References 4 publications

Epidemiological Findings on Yersiniosis in Nonhuman Primates in Zoological Gardens in Japan

Epidemiological Findings on Yersiniosis in Nonhuman Primates in Zoological Gardens in Japan

Behavior ofYersinia enterocoliticain Foods

Immuno-Magnetic Separation and Agar Layer Methods for the Isolation of Freeze-Injured Yersinia enterocolitica O:8 from Water

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