Molecular genetic variation and structure of Southeast Asian crocodile (Tomistoma schlegelii): Comparative potentials of SSRs versus ISSRs

Shafiei-Astani, Behnam; Ong, Alan Han Kiat; Valdiani, Alireza; Tan, Soon Guan; Yien, Christina Yong Seok; Ahmady, Fatemeh; Alitheen, Noorjahan Banu; Ng, Wei Lun; Kuar, Taranjeet

doi:10.1016/j.gene.2015.06.053

Cited by 15 publications

(9 citation statements)

References 32 publications

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“…For the PCR assays, we used 16 inter simple sequence repeat (ISSR) markers (Table 1), which are considered ideal for studies on molecular markers variation and diversity (Wang et al, 2012;Shafiei-Astani et al, 2015). The amplification reaction was performed with 6.25 µL of Go-Taq PCR mix (Promega), 1.25 µL of ISSR primer, 2.00 µL of DNA primer, and 2.75 µL of H 2 O in a final volume of 12.75 µL.…”

Section: Dna Extraction and Pcr Assays To Evaluate The Dissimilarity mentioning

confidence: 99%

Determination of the effects of two feed supplements on Drosophila melanogaster

Bolson

Alves

Victoria

et al. 2018

Neotropical Biology and Conservation

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Pleurotus spp. are considered extremely rich mushroom species from the nutritional point of view, providing immune-enhancement effects when consumed. However, few mushrooms have been tested for their phenotypic and genotypic responses in animal models to ensure the proper dosage for their use. This study aimed to evaluate the effects of two mushroom species on the reproductive capacity of Drosophila melanogaster. Pleurotus citrinopileatus Singer and Lentinus sajor-caju (Fr.) Fr. were provided separately, in specific concentrations, as feed supplements to the fruit flies. The total numbers of developed larvae, pupae, and adults were then measured for each treatment. Inter simple sequence repeats marker analysis was carried out to infer genotypic changes in the mushroom-fed flies. Our results suggest that Pleurotus spp. can cause positive changes to the flies' reproductive capacity, since Pleurotus citrinopileatus in particular accelerated the life cycle and revealed a higher genetic dissimilarity of the diet-supplemented flies.

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Section: Dna Extraction and Pcr Assays To Evaluate The Dissimilarity mentioning

confidence: 99%

Determination of the effects of two feed supplements on Drosophila melanogaster

Bolson

Alves

Victoria

et al. 2018

Neotropical Biology and Conservation

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“…Compared with RAPDs, ISSR achieving higher reproducibility and required time and money less than AFLPs. For this advantages ISSR are used in many studies, especially on genetic diversity (Shafiei-Astani et al 2015), DNA fingerprinting (Shen et al 2006). For ISSR analysis, a total of 59 alleles were detected among the seven pecan genotypes (Table 3 and Fig.2).…”

Section: Issr Analysismentioning

confidence: 99%

Molecular Evaluation of Genetic Diversity Among Seven Genotypes of Pecan (Carya Illinoinensis)

Elarabi

El-Soda

2017

Journal of Agricultural Chemistry and Biotechnology

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In this study twenty six RAPD primers and six ISSR primers were used to assess the genetic diversity among seven pecan genotypes cultivated in Egypt. Using the RAPD and ISSR analyses, 156 out of 276 and 30 out of 59 alleles, respectively, were detected as polymorphic markers among the seven pecan genotypes. The pecan genotypes were characterized by 59 genotypespecific markers i.e 45 for RAPD and 14 for ISSR that would be considered as useful markers for pecan genotypes. Eleven markers distinguished the Grazona genotype, five markers for the Cheyenne genotype and one marker for each of Desirable genotype, Mahan and Moneymaker.

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“…The production of ISSR markers, tack advantage of microsatellite sequences that are highly variable and universally distributed across the genome. So ISSR is a good genetic marker for various researches, on genetic variation or diversity [9], DNA fingerprinting [10], and phylogenetic [11].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers

El-Fath¹

2018

BMB

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Ten genetically stable fusants strains were obtained as a result of protoplast fusion technique between Bacillus sphaericus (Bs) and Bacillus thuringiensis (Bt), six of them produced from, Bs GHAI Bt 66 Ia experiment (fusants no. 1, 2, 16, 26, 31 and 34) and four fusants produced from Bs GHAI Bt I977experiment (fusants no.1, 2, 17 and 25). Two molecular marker systems, four ISSR primers (Inter-Simple Sequence Repeat) and seven SCoT primers (Start Codon Targeted Polymorphism) were employed for detection of genetic variation between the parents and their fusants. Four ISSR primers and seven SCoT detected 55and 97 amplicons, among which 92.696% and 81.948% bands were polymorphic respectively. The genetic similarity values among the species were calculated based on ISSR and SCoT profiles which ranged from 0.50 to 0.83 and 0.59 to 0.90 respectively. Based on the marker analysis, the three parents and the ten fusants were clustered into three major groups for ISSR primer and SCoT primers. Cluster analysis were performed to construct a dendrogram of the Bacillus Strains and their fusants and also reveals high genetic variation among the parents and their fusants. It could be concluded that each type of the two molecular marker approaches of DNA analysis could identify the different between the parents, and all of their fusants under investigation have probably originated from closely related ancestors and possess high degree of genetic similarity.

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Molecular genetic variation and structure of Southeast Asian crocodile (Tomistoma schlegelii): Comparative potentials of SSRs versus ISSRs

Cited by 15 publications

References 32 publications

Determination of the effects of two feed supplements on Drosophila melanogaster

Determination of the effects of two feed supplements on Drosophila melanogaster

Molecular Evaluation of Genetic Diversity Among Seven Genotypes of Pecan (Carya Illinoinensis)

Comparative Analysis of Genetic Diversity Among Bacillus Thuringiensis and Bacillus Sphaericus and Their Fusants Using Molecular Markers

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