Molecular genetics analysis of milk protein gene polymorphism of dairy cows and breeding bulls in Latvia

Grīslis, Z.; Smiltiņa, D.

doi:10.15159/ar.18.084

Cited by 4 publications

(3 citation statements)

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“…The obtained study results allow for the assumption that the observed picture is related to the specificities of the selection work with this breed at each farm and to the degree of "Holsteinization", used in breeding to enhance the parameters of dairy performance of cattle. The study results confirm the data for the distribution of allelic frequencies obtained in the analysis of the Holsteinized Black-and-White cattle, reared in Latvia (Smiltina et al, 2018). This assumption is also indirectly confirmed by the actual presence of allele A 1 (with different frequency) in the populations of Holstein cattle in different regions of the world (Yamada et al, 2021;Ivankovi´c et al, 2021;Bisutti et al, 2022).…”

Section: Resultssupporting

confidence: 86%

Comparative analysis of A1 and A2 allele detection efficiency for bovine CSN2 gene by AS-PCR methods

2023

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This study was aimed at conducting a comparative analysis of the efficiency in genotyping cattle by beta-casein locus using the allele-specific PCR methods. The results of the study have demonstrated the necessity to optimize the protocol for the use of AS-PCR to detect alleles A1 and A2. It was found that the use of non-optimized PCR protocols led to the genotyping errors, manifested regarding beta-casein locus (CSN2). The impossibility of using the touchdown PCR as an optimization instrument for AS-PCR was proven. The elaborated typing protocols were used to study the genetic structure of the cattle populations of different breeds, reared in Ukraine – Ukrainian Red-and-White dairy, Ukrainian Black-and-White dairy (two populations), and Charolais. It was found that locus CSN2 was polymorphic in all the cattle populations. The frequencies of allele A2 varied within 0.34–0.91 depending on the population of the animals, which may be conditioned by the specificities in the selection work. No deviation from the Hardy-Weinberg equilibrium was found in any investigated population of cattle.

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Section: Resultssupporting

confidence: 86%

Comparative analysis of A1 and A2 allele detection efficiency for bovine CSN2 gene by AS-PCR methods

2023

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“…For this reason, several post-PCR genotyping techniques are available to identify mutations in the nucleic acid sequences; some of them are challenging to perform such as amplification-created restriction site PCR (Mayer et al, 2021) and bidirectional allele-specific PCR (Chessa et al, 2013), or too expensive such as real time quantitative PCR (Manga and Dvořák, 2010), and thus not appropriate for screening purposes. Restriction fragment length polymorphism PCR and amplification refractory mutation system (ARMS)-PCR are, instead, more suitable methods for large-scale genotyping (Jaiswal et al, 2014;Smiltina and Grislis, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, the RFLP-PCR is the most common technique for the genotyping of allelic variants and has been successfully applied in numerous genetic studies (Ratna Kumari et al, 2008;Smiltina and Grislis, 2018). In this technique, a PCR primer pair is designed in such a way it amplifies a region containing a polymorphism which renders one variant accessible for restriction enzyme digestion and another not.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the genetic polymorphism linked to the β-casein A1/A2 alleles using different molecular and biochemical methods

Vigolo

Franzoi²,

Cendron³

et al. 2022

Journal of Dairy Science

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The 2 major subvariants of β-casein (A1 and A2), coded by CSN2 gene, have received great interest in the last decade both from the scientific community and the dairy sector due to their influence on milk quality. The consumption of the A1 variant, compared with the A2 variant, has a potential negative effect on human health after its digestion but, at the same time, its presence improves the milk technological properties. The aim of the present study was to compare the best method in terms of time required, costs, and technical engagement for the identification of β-casein A1 and A2 variants (homozygous and heterozygous animals) in milk to offer a reliable service for large-scale screening studies. Two allele-specific PCR procedures, namely RFLP-PCR and amplification refractory mutation system (ARMS-PCR), and one biochemical technique (HPLC) were evaluated and validated through sequencing. Manual and automated DNA extraction protocols from milk somatic cells were also compared. Automated DNA extraction provided better yield and purity. Chromatographic analysis was the most informative and the cheapest method but unsuitable for large-scale studies due to lengthy procedures (45 min per sample). Both allele-specific PCR techniques proved to be fast and reliable for differentiating between A1 and A2 variants but more expensive than HPLC analysis. Specifically, RFLP-PCR was the most expensive and labor-demanding among the evaluated techniques, whereas ARMS-PCR was the fastest while also requiring less technical expertise. Overall, automated extraction of DNA from milk matrix combined with ARMS-PCR is the most suitable technique to provide genetic characterization of the CSN2 gene on a large scale.

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Association of Allelic Variants A and B of the Beta-Lactoglobulin Gene with Dairy Productivity of Cattle

Parygina

Kozhevnikova

2023

Russ J Genet

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Molecular genetics analysis of milk protein gene polymorphism of dairy cows and breeding bulls in Latvia

Cited by 4 publications

References 0 publications

Comparative analysis of A1 and A2 allele detection efficiency for bovine CSN2 gene by AS-PCR methods

Comparative analysis of A1 and A2 allele detection efficiency for bovine CSN2 gene by AS-PCR methods

Characterization of the genetic polymorphism linked to the β-casein A1/A2 alleles using different molecular and biochemical methods

Association of Allelic Variants A and B of the Beta-Lactoglobulin Gene with Dairy Productivity of Cattle

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