Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis

Gharban, Hasanain; Shaeli, Sattar; Hussen, Talal

doi:10.5455/ovj.2023.v13.i1.4

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…The t -test in the GraphPad Prism Software was applied to estimate significant differences at p < 0.05 ( Gharban et al ., 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Characterization of β-glucan obtained from Candida albicans of caprine mastitis

Mahmoud,

Yassein

2024

Open Vet J

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Background: Beta-glucan (β-glucan) is a polysaccharide containing β-glycosidic bonds that is an important structure part of different yeast cells. Aim: The purpose of the study is to characterize β-glucan that obtained from Candida albicans (C. albicans) isolated from caprine mastitis. Methods: The β-glucan was extracted by using utilizing an Alkaline-acidic extraction technique. The dry weight of extracted β-glucan was 7.47/150 gm with 4.98%. Results: The findings demonstrated that the extracted β-glucan had similarity in the primary peak 5.78 of liquid samples using the method of High-Performance Liquid Chromatography (HPLC) when compared to the standard form of β-glucan. However, Scanning Electron Microscopy (SEM) studies revealed which the standard of β-glucan was distinct in morphology but similar to β-glucan isolated from C. albicans in terms of particle sizes in the range of 1.60-2.65 m and the lack of cell wall traces. The findings of an investigation using Energy Dispersive X-ray Fluorescence Spectroscopy (EDS/EDX) of extracted and standard β-glucan, showed the principal elements discovered were carbon (C), oxygen (O), and nitrogen (N). Aluminum (Al), silicon (Si), nickel (Ni), and gold (Au) were also present, but in less amounts. Conclusion: The extracted β-glucan displayed a high degree of similarity and purity to the standard β-glucan, according to the findings of Fourier Transform Infrared Spectroscopy (FT-IR) research.

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“…The t -test in the GraphPad Prism Software was applied to estimate significant differences at p < 0.05 ( Gharban et al ., 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Characterization of β-glucan obtained from Candida albicans of caprine mastitis

Mahmoud,

Yassein

2024

Open Vet J

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“…The tissue samples were dehydrated, cleared, infiltrated, embedded, sectioned, loaded on slides, and stained with Hematoxylin and Eosin to be examined at × 100 and × 400 of light microscope (MEIJI, Japan), [21].…”

Section: Histopathologymentioning

confidence: 99%

Histological Evaluation of Arthritis in Mice

2023

Med Clin Res

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Arthritis is one the almost commonly prevalent painful diseases which cause limit the movement of joints. This study conducts to investigate histological alterations resulted in rheumatoid arthritis (RA)-induced in mice and estimating the levels of some serum biomarkers including serum IL-6, CRP and RF. Totally, 30 were purchased, acclimated, and divided into two groups; control that injected distilled water, and arthritis that injected adjuvant (CFA). Post the 25th day of experiment, all study mice of both groups were subjected for collection of blood and right ankle joints. Serologically, the findings of IL-6, CRP and RF of arthritis group were elevated significantly when compare to control mice. Histologically, the findings observed in mice of control group were included the proliferation of chondrocytes of articular cartilage with moderate cellular and subchondral marrow space, infiltration few inflammatory cells in dermis, proliferation of chondrocytes in articular cartilage with absence of inflammatory reaction in synovial cavity, moderate infiltration of inflammatory cells in subcutaneous tissue, nearly normal articular cartilage with regular tidemark and absence of sclerosis in marrow spaces, regenerative articular cartilage and regular tidemark line with presence of very narrow distance between articular cartilage and marrow bone, and existence of normal bone trabeculae. In arthritis mice, there were an infiltration of inflammatory cells in subcutaneous tissues and dermis, marked increases in numbers of osteoclasts, sclerosis, regular tidemark and proliferation of chondrocytes, low amonunt of abscesses in subcutaneous tissue, thickness of bone trabeculae, fragments of bone, subchondral sclerosis, moderate distance between bone and articular cartilage. In conclussion, RA causes severe impacts on tissues of affected joints. Studying the mechanism of inhibition during different inflammatory process appear needed.

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Occurrence and molecular phylogeny of Fasciola species in camels of southwestern Iraq

Essa,

Azzal

2024

Vet World

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Background and Aim: Fasciola spp. are important trematodes of public health concern in various animals, including camels. This study aimed to determine the occurrence of liver flukes in camels, to determine the molecular confirmation of Fasciola, and to perform phylogenetic analysis of study isolates to identify the species of Fasciola. Materials and Methods: In total, 107 slaughtered camels were inspected to collect liver flukes that were examined molecularly using polymerase chain reaction (PCR) to confirm Fasciola species. Then, the study isolates were sequenced, submitted to the National Center for Biotechnology Information (NCBI) database, and analyzed phylogenetically to identify the species of each study isolate. Results: Liver flukes were detected in 17.67% of the camels. Regarding the migratory stages of the collected worms, juvenile worms (73.91%) were significantly more prevalent than adult worms (26.09%). Regarding the risk factors, a significantly greater occurrence rate and risk of infection was detected in Al-Najaf compared with Al-Muthanna, as well as in younger camels (1–4 years) compared with older camels. Although the occurrence rate of liver flukes was insignificantly different between females (9.38%) and males (6.98%), females appeared to be at a significantly higher risk of infection than males. Molecularly, 33.33% of the worm samples were positive for species in the Fasciola genus. Phylogenetic analysis of all positive PCR products (total no = 19) confirmed that 63.16% of the local Fasciola spp. isolates were related to the NCBI-Basic Local Alignment Search Tool (NCBI-BLAST) Saudi Arabian Fasciola hepatica isolate at an identity range of 95.94%–99%; while 36.84% of the local Fasciola spp. isolates were related to the NCBI-BLAST Iranian F. gigantica isolate at an identity range of 97.73%–99%. Conclusion: This study found a 17.67% occurrence of Fasciola spp. in camels, with juvenile worms being more common than adult worms. Molecular analysis revealed that 63.16% of the isolates were related to F. hepatica from Saudi Arabia, while 36.84% matched F. gigantica from Iran. Younger camels and those from Al-Najaf were at higher risk, highlighting the need for targeted control measures. Keywords: camel trematode, conventional polymerase chain reaction, fascioliasis, one-humped camel, phylogenetic analysis.

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Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis

Cited by 3 publications

References 58 publications

Characterization of β-glucan obtained from Candida albicans of caprine mastitis

Characterization of β-glucan obtained from Candida albicans of caprine mastitis

Histological Evaluation of Arthritis in Mice

Occurrence and molecular phylogeny of Fasciola species in camels of southwestern Iraq

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