Molecular haplotyping of tandem single nucleotide polymorphisms by allele-specific PCR

Cañadas, Carmen; Sánchez-de-Abajo, Ana; Fernández, Juan Manuel; Martín, M Carmen; Díaz-Rubio, Eduardo; Caldés, Trinidad; Hoya, Miguel de la

doi:10.1016/j.ab.2007.02.032

Cited by 1 publication

(1 citation statement)

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“…Most common methods for molecular haplotype identification are based on either amplification of one DNA molecule or separation of DNA strands by using allele‐specific amplification, cloning, hybridization, or other means. The products of amplification may be then analyzed by capillary electrophoresis, 281,282 mass spectrometry, 283 melting curve analysis, 284,285 hybridization, microarrays, 286 pyrosequencing, and microchip electrophoresis. Among other detection approaches that were used for molecular haplotyping are a combination of liquid chromatography and electrospray ionization time‐of‐flight mass spectrometry, 287 bead‐based approach, 288 linking emulsion PCR, 289 and the combination of atomic force spectrometry and carbon nanotubes 290 .…”

Section: Development Of Related Techniquesmentioning

confidence: 99%

Search for genetic markers and functional variants involved in the development of opiate and cocaine addiction and treatment

Yuferov

Levran

Proudnikov

et al. 2010

Annals of the New York Academy of Sciences

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Addiction to opiates and illicit use of psychostimulants is a chronic, relapsing brain disease that, if left untreated, can cause major medical, social and economic problems. This article reviews recent progress in studies of association of gene variants with vulnerability to develop opiate and cocaine addictions, focusing primarily on genes of the opioid and monoaminergic systems. In addition, we provide the first evidence of a cis-acting polymorphism and a functional haplotype in the PDYN gene, of significantly higher DNA methylation rate of the OPRM1 gene in the lymphocytes of heroin addicts, and significant differences in genotype frequencies of three single nucleotide polymorphisms of the P-glycoprotein gene (ABCB1) between “higher” and “lower” methadone doses in methadone-maintained patients. In genome-wide and multi-gene association studies, we have found association of a number of new genes and new variants of known genes with heroin addiction. Finally, we have described the development and application of a novel technique: molecular haplotyping for studies in genetics of drug addiction.

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