Molecular Heterogeneity Has a Major Impact on the Measurement of Circulating N-Terminal Fragments of A- and B-Type Natriuretic Peptides

Ala-Kopsala, Minna; Magga, Johanna; Peuhkurinen, Keijo; Leipälä, Jaana; Ruskoaho, Heikki; Leppäluoto, Juhani; Vuolteenaho, Olli

doi:10.1373/clinchem.2004.032490

Cited by 147 publications

(137 citation statements)

References 42 publications

Supporting

Mentioning

119

Contrasting

Unclassified

Order By: Relevance

“…However, values are assay-specific with limited comparability (11,12). In addition, cross-reactivity with pro-BNP, the precursor form with six-to eightfold lower biological activity, and glycosylated forms of BNP has been demonstrated (13).…”

Section: Discussionmentioning

confidence: 99%

Circulating N-terminal pro-B-type natriuretic peptide in fetal anemia before and after treatment

et al. 2012

View full text Add to dashboard Cite

Background: N-terminal pro-B-type natriuretic peptide (ntproBNP) is an established marker of heart failure in adult cardiology. We analyzed nt-proBNP in the circulation of fetuses with increased volume load secondary to anemia and investigated the effect of treatment on nt-proBNP concentration. Methods: Fetuses undergoing intrauterine transfusion (IUT) were examined. nt-proBNP was measured before IUT and correlated with hemoglobin concentrations, ultrasonographic findings, and Doppler measurements of the peak systolic velocity of the middle cerebral artery (Mca-PsV). Results: a total of 27 patients (7 with hydrops) and 78 controls were examined. nt-proBNP was markedly elevated in anemia (P < 0.001). concentrations were highest in hydropic fetuses (P < 0.03); no differences were present in hemoglobin and Mca-PsV values between hydropic and nonhydropic cases. In fetuses undergoing multiple IUTs nt-proBNP normalized after the third IUT, whereas hemoglobin and Mca-PsV remained abnormal. Conclusion: Levels of circulating nt-proBNP correlate well with the degree of myocardial workload in the hyperdynamic state of fetal anemia. We hypothesize that normalization of nt-proBNP after serial transfusions is an indicator of myocardial adjustment to chronic anemia. nt-proBNP measurement may be useful in the management of fetal anemia, particularly in cases at risk of hydrops and fetuses requiring multiple transfusions.

show abstract

Section: Discussionmentioning

confidence: 99%

Circulating N-terminal pro-B-type natriuretic peptide in fetal anemia before and after treatment

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Possible explanations for these different results could be differences in number and age of patients or characteristics or assay-related issues. The present assay for proANP was designed to the mid region of the prohormone, which is reportedly more stable than the N-or C-terminal part of the precursor [45]. Other proANP assays may underestimate the release of the precursor due to an early degradation of crucial epitopes at the extreme ends of the molecule.…”

Section: Discussionmentioning

confidence: 99%

Circulating levels of pro‐atrial natriuretic peptide in lower respiratory tract infections

Müller

Süess

Schuetz

et al. 2006

Journal of Internal Medicine

View full text Add to dashboard Cite

show abstract

“…The whole blood was centrifuged and plasma immediately frozen and stored at À701C. Circulating NT-proBNP (antiserum to NT-proBNP [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] ) and NT-proANP (antiserum to NT-proANP 46-79 ) concentrations were determined by radioimmunoassays as previously described. Molecular heterogeneity has a major impact on the measurement of circulating N-terminal fragments of A-and B-type natriuretic peptides.…”

Section: Natriuretic Peptidesmentioning

confidence: 99%

Very acute cardiac toxicity during BEAC chemotherapy in non-Hodgkin's lymphoma patients undergoing autologous stem cell transplantation

Kuittinen

Husso-Saastamoinen

Sipola

et al. 2005

Bone Marrow Transplant

View full text Add to dashboard Cite

Summary:Cardiotoxicity is potentially the most threatening nonhaematological side effect of high-dose CY. We prospectively evaluated the very acute cardiac effects of high-dose CY in 17 adult non-Hodgkin's lymphoma (NHL) patients receiving CY 1500 mg/m 2 /day as a part of BEAC high-dose therapy (HDT). Magnetic resonance imaging (MRI) and plasma natriuretic peptide (NT-proBNP, NT-proANP) measurements were performed prior to HDT (d-7) and just after completing HDT (d-2). After the high-dose CY left atrial end-systolic area increased from 15.271.2 to 18.571.4 cm 2 (P ¼ 0.001), left ventricular end-diastolic volume from 136.1712.3 to 156.6711.1 cm 3 (P ¼ 0.04) and left ventricular end-systolic volume from 67.477.8 to 75.377.1 cm 3 (P ¼ 0.018). However, no significant change in left ventricular ejection fraction (LVEF) was observed. At the same time, plasma levels of NT-proBNP increased from 134.9753.3 to 547.17168.4 pmol/l (P ¼ 0.003) and NT-proANP from 481.17105.5 to 1056.67193.1 pmol/l (P ¼ 0.001), respectively. To conclude, high-dose CY results in very acute cardiac toxicity characterised by enlargement of the heart chambers in NHL patients previously treated with anthracyclines. This toxicity can be detected with increased concentrations of circulating natriuretic peptides but not with LVEF measurement.

show abstract

Molecular Heterogeneity Has a Major Impact on the Measurement of Circulating N-Terminal Fragments of A- and B-Type Natriuretic Peptides

Cited by 147 publications

References 42 publications

Circulating N-terminal pro-B-type natriuretic peptide in fetal anemia before and after treatment

Circulating N-terminal pro-B-type natriuretic peptide in fetal anemia before and after treatment

Circulating levels of pro‐atrial natriuretic peptide in lower respiratory tract infections

Very acute cardiac toxicity during BEAC chemotherapy in non-Hodgkin's lymphoma patients undergoing autologous stem cell transplantation

Contact Info

Product

Resources

About