Molecular hydrogen is comparable to sulfasalazine as a treatment for DSS-induced colitis in mice

LeBaron, Tyler W.; Asgharzadeh, Fereshteh; Khazaei, Majid; Kura, Branislav; Tarnava, Alex; Slezák, J

doi:10.17179/excli2021-3762

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…Ingestion of HRW leads to a peak plasma and breath H 2 concentration in 5–15 min and returns to baseline in approximately 60 min [ 6 ]. Ingestion of HRW was shown to be comparable to sulfasalazine in a mouse model of colitis [ 7 ]. Furthermore, HRW prevented oxidative stress-induced liver fibrogenesis in mice [ 8 ] and activated peroxisome proliferator-activated receptor (PPAR) alpha and PPAR gamma expression in hepatocytes [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Effects of Hydrogen Water on Subjects with NAFLD: A Randomized, Placebo-Controlled Trial

et al. 2022

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Non-alcoholic fatty liver disease (NAFLD) is a liver pathology affecting around 25% of the population worldwide. Excess oxidative stress, inflammation and aberrant cellular signaling can lead to this hepatic dysfunction and eventual carcinoma. Molecular hydrogen has been recognized for its selective antioxidant properties and ability to attenuate inflammation and regulate cellular function. We administered hydrogen-rich water (HRW) to 30 subjects with NAFLD in a randomized, double-blinded, placebo-controlled manner for eight weeks. Phenotypically, we observed beneficial trends (p > 0.05) in decreased weight (≈1 kg) and body mass index in the HRW group. HRW was well-tolerated, with no significant changes in liver enzymes and a trend of improved lipid profile and reduced lactate dehydrogenase levels. HRW tended to non-significantly decrease levels of nuclear factor kappa B, heat shock protein 70 and matrix metalloproteinase-9. Interestingly, there was a mild, albeit non-significant, tendency of increased levels of 8-hydroxy-2’-deoxyguanosine and malondialdehyde in the HRW group. This mild increase may be indicative of the hormetic effects of molecular hydrogen that occurred prior to the significant clinical improvements reported in previous longer-term studies. The favorable trends in this study in conjunction with previous animal and clinical findings suggest that HRW may serve as an important adjuvant therapy for promoting and maintaining optimal health and wellness. Longer term studies focused on prevention, maintenance, or treatment of NAFLD and early stages of NASH are warranted.

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Section: Introductionmentioning

confidence: 99%

Biological Effects of Hydrogen Water on Subjects with NAFLD: A Randomized, Placebo-Controlled Trial

et al. 2022

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“…Previous studies showed that the colon length was negatively correlated with the severity of colitis. [ 16 17 ] As shown in [ Figure 2 ], evaluation of colon length revealed that the colitis group exhibited significantly shorten colon and reduced colon length compare to control group ( p < 0.01). Colon weight to length ratio which is a marker of inflammation was significantly higher in colitis group compare with control group ( p < 0.05).…”

Section: Resultsmentioning

confidence: 94%

Exercise Improves Clinical Symptoms, Pathological Changes and Oxidative/Antioxidative Balance in Animal Model of Colitis

Kolahi,

Yaghoubi,

Rezaeian

et al. 2023

International Journal of Preventive Medicine

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Background: Ulcerative colitis is one of the major phenotypic forms of inflammatory bowel diseases. The present study aimed to investigate the effect of force swimming exercise on clinical symptoms (disease activity index; DAI), colon histopathology, inflammation and fibrosis, and oxidant/antioxidant balance in dextran sulfate sodium (DSS)-induced colitis mice. Methods: Male C57BL6 mice were randomly divided into five groups ( n = 6 each): control, exercise, colitis, colitis + sulfasalazine, and colitis + exercise. Exercise was performed by forced swimming six weeks before and during the experiment. Colitis was induced by 1.5% DSS in drinking water. The animals were evaluated for body weight changes and DAI (including changes of body weight, stool consistency, rectal bleeding, and prolapse) during the induction of colitis and treatment. At the end of experiment, colons and spleens were evaluated by H and E and Masson Trichrome stainings. Oxidant (Malon dialdehyde; MDA), and antioxidant markers [total thiol groups, superoxide dismutase (SOD), and catalase activity] were also measured in colon tissue. Results: Results indicated that exercise in colitis mice significantly improved DAI, colon length, spleen weight, and histological injury score and alleviated fibrotic changes in colon tissue that were comparable to sulfasalazine group. Exercise also restored the oxidant/antioxidant balance in colitis mice by reducing MDA and increasing antioxidative markers including total thiol groups, SOD, and catalase activity. Conclusions: Taken together, aerobic exercise could improve clinical symptoms and colonic inflammation through, at least, the balancing the oxidative stress markers. Thus, it can be considered in management of colitis patients as effective method.

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“…Sulfasalazine is commonly used for the treatment of mild to moderate UC, but there will be anorexia, itching, headache, stomach pain, and other adverse reactions. In the studies of mice with UC, sulfasalazine was often used as the positive control group. , …”

Section: Methodsmentioning

confidence: 99%

“…In the studies of mice with UC, sulfasalazine was often used as the positive control group. 20,21 The animal experimental procedure is described in Figure 1. Thirty-two mice were randomly divided into four groups, with eight mice per group: control group (CN), DSS group (MD), sulfasalazine group (PC), and FAEb group (FFJ).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Effect of Lactobacillus with Feruloyl Esterase-Producing Ability on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice

Shen

et al. 2022

J. Agric. Food Chem.

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Ulcerative colitis (UC) is becoming an increasingly serious health problem. This study aimed to investigate the effect of a newly isolated Lactobacillus species that produces feruloyl esterase (FAEb) on dextran sodium sulfate (DSS)-induced UC in mice. In this study, FAEb supplementation slowed body weight loss and mitigated colon length shortening, the severity of fecal occult blood, and increases in the disease activity index (DAI) in UC model mice. FAEb supplementation was also shown to reduce the expression of proinflammatory factors, increase the antioxidant capacity, improve the production of beneficial short-chain fatty acids (SCFAs), upregulate the expression of tight junction proteins, reduce the histopathological scores, and reduce mucous barrier damage in the gut. Furthermore, FAEb supplementation was shown to inhibit inflammatory NF-κB signaling pathway activity, increase the abundance of beneficial bacteria, and regulate the balance of microbiota in the gut. These results suggest that FAEb may serve as a potential probiotic to prevent and treat UC.

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Molecular hydrogen is comparable to sulfasalazine as a treatment for DSS-induced colitis in mice

Cited by 8 publications

References 28 publications

Biological Effects of Hydrogen Water on Subjects with NAFLD: A Randomized, Placebo-Controlled Trial

Biological Effects of Hydrogen Water on Subjects with NAFLD: A Randomized, Placebo-Controlled Trial

Exercise Improves Clinical Symptoms, Pathological Changes and Oxidative/Antioxidative Balance in Animal Model of Colitis

Effect of Lactobacillus with Feruloyl Esterase-Producing Ability on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice

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