Molecular identification and antibiotic control of endophytic bacterial contaminants from micropropagated Aglaonema cultures

Fang, Jong-Yi; Hsu, Yu-Rong

doi:10.1007/s11240-012-0129-6

Cited by 36 publications

(24 citation statements)

References 47 publications

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“…Antibiotic pretreatment was essential for the establishment of aseptic culture in Aglaonema 'Lady Valentine,' as documented previously (Fang and Hsu 2012). Thereafter, two PGRs (i.e.…”

Section: Discussionmentioning

confidence: 83%

“…The explants were surface-sterilized in 70% ethanol for 1 min followed by 1% sodium hypochlorite (NaOCl), containing several drops of Tween-20, for 20 min. After three rinses with sterile distilled water and removal of the damaged ends, the explants were individually cultured in 20×150 mm test tubes containing 10 ml semi-solid Murashige and Skoog (MS) (Murashige and Skoog 1962) medium supplemented with 32 mg·l −1 gentamicin, 8 mg·l −1 tetracycline and 4 mg·l −1 chloramphenicol according to Fang and Hsu (2012). The basal medium consisted of full-strength MS salts and vitamins, 3% (w/v) sucrose (Bio Basic Inc., Canada) and 0.8% (w/v) agar (Bio Basic Inc., Canada).…”

Section: Aseptic Culture Establishmentmentioning

confidence: 99%

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Development of an efficient micropropagation procedure for Aglaonema ^|^#8216;Lady Valentine^|^#8217; through adventitious shoot induction and proliferation

Fang

Hsu

Chen

2013

Plant Biotechnology

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An efficient micropropagation procedure via adventitious shoot proliferation was developed for Aglaonema using the popular red cultivar 'Lady Valentine. ' Aseptic culture was initiated by culturing stem nodal segments on Murashige and Skoog (MS) medium supplemented with 32 mg·l −1 gentamicin, 8 mg·l −1 tetracycline and 4 mg·l −1 chloramphenicol. The growth of the axillary buds performed the best when 10 mg·l −1 6-benzyladenine (BA) was incorporated into the medium, and neither gibberellic acid (GA 3 ) nor dark exposure could improve the elongation of the axillary shoots. The single stem nodal segments excised from the elongated shoots were treated with different combinations of α-naphthaleneacetic acid (NAA) and thidiazuron (TDZ) and an average of 10.9 adventitious shoots per stem segment was produced with 0.5 mg·l −1 NAA and 2 mg·l −1 TDZ. Small shoot clusters were subsequently incubated with different concentrations of BA and GA 3 and results showed that 0.5-5 mg·l −1 BA treatments were more effective for shoot proliferation and elongation than 0.5-1 mg·l −1 GA 3 treatments. The longest shoots (reaching 2.69 cm after three months) were obtained on medium containing 5 mg·l −1 BA. Up to 80% of the elongated shoots successfully rooted ex vitro with the application of 1 and 2 mg·l −1 indole-3-butyric acid (IBA) and 92.5% of these rooted shoots survived following transfer to the greenhouse.

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“…Antibiotic pretreatment was essential for the establishment of aseptic culture in Aglaonema 'Lady Valentine,' as documented previously (Fang and Hsu 2012). Thereafter, two PGRs (i.e.…”

Section: Discussionmentioning

confidence: 83%

Section: Aseptic Culture Establishmentmentioning

confidence: 99%

Development of an efficient micropropagation procedure for Aglaonema ^|^#8216;Lady Valentine^|^#8217; through adventitious shoot induction and proliferation

Fang

Hsu

Chen

2013

Plant Biotechnology

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“…Microbial contaminants may arises from different sources like infected plant materials, improper tissue culture technique and poor laboratory condition 23,24,25,26,27 . Explant contamination is related to several factors like source of explants and environment 21 .…”

Section: Biotic Contamination In Plant Tissue Culturementioning

confidence: 99%

“…All the earlier works, researchers used broad spectrum antibiotics for decontamination. Reports on application of antibiotic after identifying the bacteria,are available in case of Ilex dumosa 55 , Aglaonema 27 , Guadua angustifolia Kunth 64 . No attempts have been taken to use antibiotic after identifying bacteria in bamboo (except in Guadua angustifolia Kunth).…”

Section: Antibiotic As Surface Sterilants In Bamboomentioning

confidence: 99%

Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation.

Ray

Ali

2018

Braz. arch. biol. technol.

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Multipurpose use of bamboo in rural life makes it as poor man's timber in Asian countries. Deforestation and industrialization leads to destruction of natural forest. To replenish, a rapid plantation of bamboo could be one of the possible solutions. Bamboo is propagated mainly by vegetative methods though it is not suitable for large scale plantation because of several limitations. Micropropagation is gaining importance for large scale propagation because of its capability in raising huge number of true to type propagules in a limited space in very short span of time. Like any other plant, the chief constraint of bamboo micropropagtion is in vitro contamination arises from several sources including explants. Most of the contaminants are reduced by maintaining aseptic conditions. The surface adhering microbial contaminant (Epiphytic) is usually checked by using several available surface sterilants. But the endophytic contaminant (present within the explants) is not easily controlled. Endophytic fungus could be controlled by using systemic fungicides but controlling bacteria is again more troublesome. Antibiotic with broad spectrum activity coupled with low phytotoxicity is prerequisite to get better results. Treatment duration and type of antibiotic are the critical factor to reduce the contamination. But unscientific use of antibiotic may lead to the development of resistant microbial strains. That is why antibiotic selection after identification of the contaminants may be an efficient way to counter this problem. The present review is done on use of antibiotic controlling bacterial contamination during micropropagation special reference to bamboo.

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“…Isto significa que o processo de micropropagação, ao envolver condições ambientais tão restritas, passa a ser o responsável por alterações significativas na comunidade bacteriana associada ao vegetal, criando um ambiente exclusivo, de modo a selecionar grupos bacterianos benéficos ou neutros, mais ou menos adaptados ao ambiente in vitro (FANG;HSU, 2012;QUAMBUSCH et al, 2014).…”

Section: Introductionunclassified

Estudo da comunidade bacteriana endofítica e de sua manifestação na micropropagação de Eucalyptus benthamii

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Molecular identification and antibiotic control of endophytic bacterial contaminants from micropropagated Aglaonema cultures

Cited by 36 publications

References 47 publications

Development of an efficient micropropagation procedure for Aglaonema ^|^#8216;Lady Valentine^|^#8217; through adventitious shoot induction and proliferation

Development of an efficient micropropagation procedure for Aglaonema ^|^#8216;Lady Valentine^|^#8217; through adventitious shoot induction and proliferation

Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation.

Estudo da comunidade bacteriana endofítica e de sua manifestação na micropropagação de Eucalyptus benthamii

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