Molecular identification and antibiotic susceptibility patterns of Escherichia coli isolates from sheep faeces samples

Saei, Habib Dastmalchi; Ahmadi, Elham; Kazemnia, Ali; Ahmadinia, Mehrzad

doi:10.1007/s00580-010-1134-y

Cited by 11 publications

(8 citation statements)

References 40 publications

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“…In Spain, O157:H7 STEC isolated from sheep, cattle and humans were detected, with a resistance profile of 20% (1/5) of ovine and 53% (42/82) of cattle; in bovine STEC, Mora 19%), in agreement with our results. Also similarly to our study, Saei et al (2012) reported low resistance to the antibiotics tetracycline (10%) and gentamicin (4%). According to the phylogenetic classification, 24.4% (22/90) were from group D (pathogenic), 32.2% (29/90) from group B1 (commensal) and 43.3% (39/90) from group A (commensal).…”

Section: Discussionsupporting

confidence: 90%

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Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

et al. 2015

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Section: Discussionsupporting

confidence: 90%

“…In the last few years, the number of pathogenic isolates with antimicrobial multiresistance has increased, which narrows the options of antibiotics to treat human infections (Saei et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

et al. 2015

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“…In the present study, ESBL antibiotic resistance genes (blaCTX-M, blaTEM and blaOXA) were selected based on their resistance against cefotaxime, amoxicillin-clavulanic acid and oxacillin respectively. Widespread β-lactam antibiotic prescription in the field of veterinary medicine is one of the factors causing the presence of ESBL antibiotic-resistant bacteria to reach epidemic proportions in recent years [60,61].…”

Section: Discussionmentioning

confidence: 99%

Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt

Hussein

Eid

Ibrahim

et al. 2019

Zagazig Veterinary Journal

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Colibacillosis is a complicated disease causing severe economic losses and challenging veterinarians and producers. Therefore, this study aimed to characterize avian pathogenic Escherichia coli (APEC) strains causing respiratory signs in chickens. Thirty broiler chicken flocks at age of 17-35 days showed respiratory signs and greenish diarrhea during 2013-2016 outbreaks that occurred in Sharkia, Ismailia, Dakahlia and Sinai Governorates. The postmortem findings revealed typical colisepticemia picture including air sacculitis, fibrinous pericarditis and perihepatitis. The percentage of APEC isolation was 100%. Mixed bacterial infections with Enterobacter aerogenes or Providencia rettgeri (3 flocks, each), Klebsiella pneumoniae (2 flocks), Serratia liquefaciens or Enterobacter agglomerans (1 flock, each) was evidenced. From 284 collected samples (air sacs, heart blood, lungs and liver), E. coli was predominantly isolated from air sacs (76.1 %) and lung (73.2 %) followed by heart blood (67.6%) and liver (54.9%). Based on serogrouping, the most common serogroups were O78 and O2 with percentages of 15%, each. Utilizing antimicrobial disc diffusion test, the isolates showed 32.7% resistance to doxycycline and 100% resistance to lincomycin, spiramycin, oxacillin and amoxicillin. Polymerase chain reaction (PCR) analysis for 55 MDR E. coli isolates from air sac and heart blood revealed 3 β-lactamase resistance genes; blaTEM (87.3 %), blaCTX-M (85.5 %) and blaOXA (5.5 %) and 6 virulence genes in two multiplex PCR; iucD (96.4%), Fim H (92.7%), iss (76.4%), ompT (58.2%), tsh (45.5%) and cvaC (9.1%). An association of virulence with multidrug resistance genes in E. coli was recorded, that hindered the control measures. Therefore, alternative strategies were necessary to minimize the antibiotic use and reduce the virulent strains' occurrence.

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“…Furthermore, the enterohemolysins are encoded by hylA or ehxA genes and that enhance the effect of Shiga toxins (Croxen et al, 2013). In the last few years, there is an increased pathogenicity and resistance to most antimicrobials conferred by E. coli isolates that lower the selection chances of effective antimicrobial to control human infections (Saei et al, 2012).…”

Section: Research Articlementioning

confidence: 99%

Virulence Repertoire and Antimicrobial Resistance Profile of Shiga Toxin-Producing E.coli Isolated from Sheep and Goat Farms from Al-buhayra Egypt

El-Sayed¹

2018

PVJ

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Shiga toxin-producing E.coli (STEC) evokes a paramount concern from the public health point of view. Many reports dealt with the characterization of STEC from large ruminants. This study aimed to investigate the presence of STEC in sheep and goats, distribution of stx1, stx2, eaeA, and hlyA genes encoding Shiga toxins, intimin, enterohemolysins, and the antimicrobial resistance index (MAR). A total of 170 samples collected from (diarrheic, apparently healthy, and milk samples) from sheep, goats, and bedding (136, 27 and 7) respectively. E.coli was detected at a rate of 71 (41.7%) distributed as 62 (44%) and 9 (31%) from sheep and goat, respectively. The prevalent serotypes were O111:

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Molecular identification and antibiotic susceptibility patterns of Escherichia coli isolates from sheep faeces samples

Cited by 11 publications

References 40 publications

Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt

Virulence Repertoire and Antimicrobial Resistance Profile of Shiga Toxin-Producing E.coli Isolated from Sheep and Goat Farms from Al-buhayra Egypt

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