Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds

Abstract

Background The discovery of potential, new cost-effective drug resources in the form of bioactive compounds from mushrooms is one way to control the resistant pathogens. In the present research, the fruiting bodies of five wild mushrooms were collected from Egypt and identified using internal transcribed spacer region (ITS) of the rRNA encoding gene and their phylogenetic relationships, antimicrobial activities, and biochemical and phenolic compounds were evaluated. Results The sequences revealed identity to Bjerkandera adusta, Cyclocybe cylindracea, Agrocybe aegerita, Chlorophyllum molybdites, and Lentinus squarrosulus in which Cyclocybe cylindracea and Agrocybe aegerita were closely related, while Chlorophyllum molybdites was far distant. Cyclocybe cylindracea and Agrocybe aegerita showed 100% similarity based on the sequenced ITS-rDNA fragment and dissimilar antimicrobial activities and chemical composition were detected. Bjerkandera adusta and Cyclocybe cylindracea showed strong antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Micrococcus luteus, Streptococcus pneumoniae, and Candida albicans. This activity could be attributed to the detected phenolic and related compounds’ contents. Conclusion Our finding provides a quick and robust implement for mushroom identification that would facilitate mushroom domestication and characterization for human benefit.

Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

“…An HPLC-DAD-MS analysis was also carried out in order to measure the levels of selected phenolic and flavonoid compounds, namely, gallic acid, hydroxytyrosol, catechin, chlorogenic acid, epicatechin and benzoic acid, that play a major role in the antioxidant/antimicrobial response following mushroom extract administration [40,41]. Specifically, Table 1 shows that the level of gallic acid is higher in extracts A and C, whereas extract B do not show a relevant amount of this compound.…”

Enhanced Efficiency of the Removal of Cytostatic Anthracycline Drugs Using Immobilized Mycelium of Bjerkandera adusta CCBAS 930

“…The lack of correlations in the content of these compounds with absorbance increases at 240 nm-250 nm [44] suggested that their biosynthesis was associated with the metabolism of B. adusta CCBAS 930. Earlier studies have shown that fungi, e.g., Bjerkandera adusta, synthesize phenolic compounds [45,46], and that the addition of exogenic phenolic acids increases the efficiency of the production of phenolic compounds [47]. Moreover, during the biotransformation of anthraquinone derivatives by B. adusta CCBAS 930, an increase in the content of phenolic compounds was observed [8].…”

Safe production of Aspergillus terreus xylanase from Ricinus communis: gene identification, molecular docking, characterization, production of xylooligosaccharides, and its biological activities

“…20 Phylogenetic tree of xylanase proteins from A. terreus strain RGS. Eg-NRC was inferred using the neighbor-joining method (MEGA X) gene for fungal identification [12]. For example, a previous study [44] employed the ITS gene for the tion of some indigenous Beauveria isolates.…”

“…Hence, to help with the production process of traditional meals and minimize toxins in various substrates, microorganisms (fungi), for example, Aspergillus terreus (A. terreus), have been implemented However, although some strains of A. terreus generate mycotoxins, which are toxic to both animals and humans [11], a preliminary assessment of mycotoxin production in Aspergillus was conducted. Their results showed nonexistent mycotoxin production [12]. Also, fermentation methods can improve food safety by lowering harmful chemicals during manufacturing [13].…”