Molecular identification and antimicrobial activity of endophytic fungi isolated from Heritiera fomes (Buch. -Ham), a mangrove plant of the Sundarbans

Nurunnabi, Tauhidur Rahman; Sarwar, Sabiha; Sabrin, Farah; Alam, Ferdous; Nahar, Lutfun; Sohrab, Hossain; Sarker, Satyajit D.; Rahman, Suzanah Abdul; Billah, Morsaline

doi:10.1186/s43088-020-00081-9

Cited by 5 publications

(7 citation statements)

References 29 publications

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“…SA2, and Aspergillus sp. SA3 results in annotation of 26 compounds (1-7), (8)(9)(10)(11)(12)(13)(14)(15)(16)(17) and (18)(19)(20)(21)(22)(23)(24)(25)(26) respectively, as shown in Table 1; Fig. 4.…”

Section: Resultsmentioning

confidence: 97%

“…Therefore, finding new antibiotics is crucial to combat these resistant bacteria [10]. Natural compounds from endophytic fungi are potential candidates for new antibiotics against pathogenic bacteria [11]. Therefore, in recent years, more attention has been given to the endophytic fungi of medicinal plants, which represent a rich source of new and useful natural compounds of interest to the pharmaceutical and agricultural industries and a potential source for the discovery of novel microorganisms [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical analysis and anti-infective potential of fungal endophytes isolated from Nigella sativa seeds

Shady,

Sobhy,

Mostafa

et al. 2023

BMC Microbiol

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Endophytic fungi, particularly from higher plants have proven to be a rich source of antimicrobial secondary metabolites. The purpose of this study is to examine the antimicrobial potential of three endophytic fungi Aspergillus sp. SA1, Aspergillus sp. SA2, and Aspergillus sp. SA3, cultivated from Nigella sativa seeds against Staphylococcus aureus (ATCC 9144), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 13883), MRSA (ATCC 33591), and human pathogen Candida albicans (ATCC 10231). Furthermore, the most active cultivated endophytic fungi were molecularly identified via internal transcribed spacer (ITS) sequencing. HR-ESIMS guided approach has been used successfully in chemical profiling of 26 known bioactive secondary metabolites (1–26), which belongs to different classes of natural compounds such as polyketides, benzenoids, quinones, alcohols, phenols or alkaloids. Finally, in-silico interactions within active site of fungal Cyp51 and bacterial DNA gyrase revealed possibility of being a hit-target for such metabolites as antimicrobials.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis and anti-infective potential of fungal endophytes isolated from Nigella sativa seeds

Shady,

Sobhy,

Mostafa

et al. 2023

BMC Microbiol

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“…Some pioneering works have been done in recent years which reported the antibacterial potential of endophytic fungi associated with the plants of Sundarbans. One such study led to the identification of kojic acid, oxysporone, and xylitol with profound antibacterial potential [39][40][41][42]. In this study, we isolated and identified three compounds produced by endophytic A. fumigatus associated with C. decandra, which showed profound activity in the antibacterial assay.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans

Zihad

Hasan

Sultana

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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The Sundarbans, a UNESCO world heritage site, is one of the largest mangrove forests in one stretch. Mangrove plants from this forest are little studied for their endophytic fungi. In this study, we isolated fourteen endophytic fungi from the plants Ceriops decandra and Avicennia officinalis collected from the Sundarbans. Five of them were identified as Aspergillus sp. and one as Penicillium sp. by macroscopic and microscopic observation. Antibacterial activity of the crude extracts obtained from these endophytes was determined against Staphylococcus aureus, Micrococcus luteus, Escherichia coli, and Pseudomonas aeruginosa using resazurin-based microtiter assay. The isolated endophytes showed varying degrees of antibacterial activity with MICs ranging between 5 and 0.078 mg/mL. Molecular identification of the most active endophyte revealed its identity as Aspergillus fumigatus obtained from the leaves of C. decandra. Acute toxicity study of the ethyl acetate extract of A. fumigatus in mice revealed no mortality even at the highest dose of 2000 mg/kg bodyweight, though some opposing results are found in the subacute toxicity study. The extract was subjected to silica gel and Sephadex column chromatography resulting in the isolation of three pure compounds. LC-MS analysis of these pure compounds revealed their identity as fumigaclavine C, azaspirofuran B, and fraxetin. This is the first report of fraxetin from A. fumigatus. All three identified compounds were previously reported for their antibacterial activity against different strains of both Gram-positive and Gram-negative bacteria. Therefore, the observed antibacterial activity of the ethyl acetate (EtOAc) extract of A. fumigatus could be due to the presence of these compounds. These results support the notion of investigating fungal endophytes from the Sundarbans for new antimicrobial compounds.

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“…Therefore, finding new antibiotics is crucial to combat these resistant bacteria [4]. Natural compounds from endophytic fungi are potential candidates for new antibiotics against pathogenic bacteria [5]. Because secondary metabolites of endophytic fungi have been reported to exhibit a number of interesting potential biological activities such as antibacterial, antifungal, cytotoxic, antivirus, anti-inflammatory, etc.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis

Eshboev,

Karakozova,

Abdurakhmanov

et al. 2023

Antibiotics

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According to the World Health Organization, it is estimated that by 2050, drug-resistant infections could cause up to 10 million deaths annually. Therefore, finding a new generation of antibiotics is crucial. Natural compounds from endophytic fungi are considered a potential source of new-generation antibiotics. The antimicrobial and cytotoxic effects of ethyl acetate extracts of nine endophytic fungal isolates obtained from Hyssopus officinalis were investigated for bioassay-guided isolation of the natural compounds. An extract of isolate VII showed the highest antimicrobial activities against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus (30.12 ± 0.20 mm and 35.21 ± 0.20 mm) and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (30.41 ± 0.23 mm and 25.12 ± 0.25 mm) among the tested extracts of isolates. Molecular identification of isolate VII confirmed it as Chaetomium elatum based on sequencing of its ITS genes, and it was discovered that this was the first time C. elatum had been isolated from H. officinalis. This isolate was cultured at a large scale for the isolation and identification of the active compound. Penicillic acid was isolated for the first time from C. elatum and its chemical structure was established by NMR spectroscopy. The penicillic acid showed strong antibacterial activities against Bacillus subtilis and Staphylococcus aureus with 20.68 mm and 25.51 mm inhibition zones, respectively. In addition, MIC and MBC values and antibiofilm activities of penicillic acid were determined. It was found that penicillic acid reduced the level of biofilms in proportion to antibacterial activity.

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Molecular identification and antimicrobial activity of endophytic fungi isolated from Heritiera fomes (Buch. -Ham), a mangrove plant of the Sundarbans

Cited by 5 publications

References 29 publications

Phytochemical analysis and anti-infective potential of fungal endophytes isolated from Nigella sativa seeds

Phytochemical analysis and anti-infective potential of fungal endophytes isolated from Nigella sativa seeds

Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans

Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant Hyssopus officinalis

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