Molecular identification and bioinformatics analysis of a potential anti-vector vaccine candidate, 15-kDa salivary gland protein (Salp15), from Ixodes affinis ticks

Sultana, Hameeda; Patel, Unnati; Toliver, Marcée; Maggi, Ricardo G.; Neelakanta, Girish

doi:10.1016/j.ttbdis.2015.08.003

Cited by 13 publications

(16 citation statements)

References 42 publications

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“…These sequences were individually analysed at PROSITE (http://prosite.expasy.org/) to predict post‐translational modifications such as PKC, CK2, cAMP and tyrosine phosphorylation sites. In addition, myristoylation, N‐glycosylation and RGD sites were determined as described (Sultana et al ., ). Hypothetical protein modelling of Otlip was performed using the Phyre‐2 fold recognition server (Kelley et al ., ) using homology with monotonin, the serotonin binding protein from the saliva of the soft tick A. monolakensis .…”

Section: Methodsmentioning

confidence: 97%

“…qRT‐PCR was performed using iQ‐SYBR Green Supermix (Bio‐Rad). A standard curve was prepared using 10‐fold serial dilutions starting from 1 to 0.00001 ng of known quantities of otlip or 28S fragments and qRT‐PCR reactions were performed as described (Sultana et al ., ; Neelakanta et al ., )…”

Section: Methodsmentioning

confidence: 99%

“…We investigated whether or not post-translational modifications and/or conserved domains were present on Otlip and other salivary lipocalins. The amino acid sequences of Otlip and other lipocalins were individually analysed at PROSITE as described (Sultana et al, , 2016. Based on the PROSITE analysis, all but three lipocalins analysed in this study carried at least one protein kinase C (PKC) phosphorylation site ( Fig.…”

Section: Prediction Of Post-translational Modifications and Domain Anmentioning

confidence: 99%

“…A standard curve was prepared using 10-fold serial dilutions starting from 1 to 0.00001 ng of known quantities of otlip or 28S fragments and qRT-PCR reactions were performed as described (Sultana et al, , 2016Neelakanta et al, 2017) Comparative analysis of post-translational modifications on Otlip and other BAB motif-containing lipocalins from soft ticks (http://prosite.expasy.org/) to predict posttranslational modifications such as PKC, CK2, cAMP and tyrosine phosphorylation sites. In addition, myristoylation, Nglycosylation and RGD sites were determined as described (Sultana et al, , 2016. Hypothetical protein modelling of Otlip was performed using the Phyre-2 fold recognition server (Kelley et al, 2015) using homology with monotonin, the serotonin binding protein from the saliva of the soft tick A. monolakensis.…”

Section: Qrt-pcr Analysismentioning

confidence: 99%

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Identification and characterization of a histamine‐binding lipocalin‐like molecule from the relapsing fever tick Ornithodoros turicata

Neelakanta

Sultana

Sonenshine

et al. 2017

Insect Molecular Biology

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Lipocalins are low molecular weight membrane transporters that are abundantly expressed in the salivary glands and other tissues of ticks. In this study, we identified a lipocalin‐like molecule, designated as otlip, from the soft ticks Ornithodoros turicata, the vector for the relapsing fever causing spirochete Borrelia turicatae. We noted that the expression of otlip was developmentally regulated, with adult ticks expressing significantly higher levels in comparison to the larvae or nymphal ticks. Expression of otlip was evident in both fed and unfed O. turicata ticks, with significantly increased expression in the salivary glands in comparison to the midgut or ovary tissues. High conservation of the biogenic amine‐binding motif was evident in the deduced primary amino acid sequence of Otlip. Protein modelling of Otlip revealed conservation of most of the residues involved in binding histamine or serotonin ligand. In vitro assays demonstrated binding of recombinant Otlip with histamine. Furthermore, prediction of post‐translational modifications revealed that Otlip contained phosphorylation and myristoylation sites. Taken together, our study not only provides evidence for the presence of a lipocalin‐like molecule in O. turicata ticks but also suggests a role for this molecule in the salivary glands of this medically important vector.

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Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Prediction Of Post-translational Modifications and Domain Anmentioning

confidence: 99%

Section: Qrt-pcr Analysismentioning

confidence: 99%

See 2 more Smart Citations

Identification and characterization of a histamine‐binding lipocalin‐like molecule from the relapsing fever tick Ornithodoros turicata

Neelakanta

Sultana

Sonenshine

et al. 2017

Insect Molecular Biology

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“…More recent research has also revealed the role of Salp15 in the pathogenesis of a range of diseases; these important findings are discussed below. Over the past two decades, an increasing number of research studies have attempted to identify and characterize Salp15 homologs in various Ixodes species (22)(23)(24)(25). The immunomodulatory effects of Salp15 present important opportunities for the development of novel and sophisticated therapies for human disease.…”

Section: Introductionmentioning

confidence: 99%

Salp15, a Multifunctional Protein From Tick Saliva With Potential Pharmaceutical Effects

Wen

Wang

et al. 2020

Front. Immunol.

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Ixodes ticks are the main vectors for a number of zoonotic diseases, including Lyme disease. Ticks secrete saliva directly into a mammalian host while feeding on the host's blood. This action serves to modulate host immunity and coagulation, thus allowing ticks to attach and feed upon their host. One of the most extensively studied components of tick saliva is Salp15. Research has shown that this protein binds specifically to CD4 molecules on the surface of T lymphocytes, interferes with TCR-mediated signaling transduction, inhibits CD4+ T cell activation and proliferation, and impedes the secretion of interleukin 2 (IL-2). Salp15 also binds specifically to dendritic cell dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) to up-regulate the expression of CD73 in regulatory T cells. Collectively, these findings render this salivary protein a potential candidate for a range of therapeutic applications. Here, we discuss our current understanding of Salp15 and the mechanisms that might be used to treat disease.

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Population genetics of the Ixodes affinis (Ixodida: Ixodidae) complex in America: new findings and a host-parasite review

Rodríguez-Vivas,

Ojeda-Chi,

Thompson

et al. 2023

Parasitol Res

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Molecular identification and bioinformatics analysis of a potential anti-vector vaccine candidate, 15-kDa salivary gland protein (Salp15), from Ixodes affinis ticks

Cited by 13 publications

References 42 publications

Identification and characterization of a histamine‐binding lipocalin‐like molecule from the relapsing fever tick Ornithodoros turicata

Identification and characterization of a histamine‐binding lipocalin‐like molecule from the relapsing fever tick Ornithodoros turicata

Salp15, a Multifunctional Protein From Tick Saliva With Potential Pharmaceutical Effects

Population genetics of the Ixodes affinis (Ixodida: Ixodidae) complex in America: new findings and a host-parasite review

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