Molecular identification and cytotoxicity study of herbal medicinal materials that are confused by Aristolochia herbs

Li, Ming; Au, Ka-Yee; Lam, Hilary; Cheng, Ling; But, Paul Pui‐Hay; Shaw, Pang‐Chui

doi:10.1016/j.foodchem.2013.09.146

Cited by 16 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As described in this paper, DNA barcoding provides a direct method to confirm target species identity and. less frequently, to detect specific toxic adulterants [ 57 , 93 , 94 , 95 , 96 ]. A major advantage of metabarcoding assays is that they provide a “What’s In My Pot?” (WIMP) analysis [ 97 ] which determines both identity and purity.…”

Section: Discussionmentioning

confidence: 99%

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Howard

Lockie-Williams

Slater

2020

Plants

View full text Add to dashboard Cite

DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharmacopoeias represents a step-change in status, requiring the establishment of standardized, reliable and reproducible methods. The process by which this can be achieved for any herbal medicine is described, using Hypericum perforatum L. (St John’s Wort) and potential adulterant Hypericum species as a case study. A range of practical issues are considered including quality control of DNA sequences from public repositories and the construction of individual curated databases, choice of DNA barcode region(s) and the identification of informative polymorphic nucleotide sequences. A decision tree informs the structure of the manuscript and provides a template to guide the development of future DNA barcode tests for herbals.

show abstract

Section: Discussionmentioning

confidence: 99%

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Howard

Lockie-Williams

Slater

2020

Plants

View full text Add to dashboard Cite

show abstract

“…Numerous studies have not only affirmed its competency for species identification, but also highlighted its strengths in defining species boundaries and flagging new species [20] [49]. Owing to its ease and swiftness, this tool has been used extensively for distinguishing medicinal plants [50] [51] [52]. It is predicted to play a significant role in identifying medicinal plant products in the future [53].…”

Section: Discussionmentioning

confidence: 99%

Emergence of Plastidial Intergenic Spacers as Suitable DNA Barcodes for Arid Medicinal Plant <i>Rhazya stricta</i>

Khan¹,

Baeshen²,

Ramadan³

et al. 2017

AJPS

View full text Add to dashboard Cite

The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this species. The authenticity of R. stricta and other medicinal plants and herbs procured from local markets can be questionable due to a lack of clear phenotypic traits. DNA barcoding is an emerging technology for rapid and accurate species identification. In this study, six candidate chloroplastid barcodes were investigated for the authentication of R. stricta. We compared the DNA sequences from fifty locally collected and five market samples of R. stricta with database sequences of R. stricta and seven closely related species. We found that the coding regions matK, rbcL, rpoB, and rpoC1 were highly similar among the taxa. By contrast, the intergenic spacers psbK-psbI and atpF-atpH were variable loci distinct for the medicinal plant R. stricta. psbK-psbI clearly discriminated R. stricta samples as an efficient single locus marker, whereas a two-locus marker combination comprising psbK-psbI + atpF-atpH was also promising according to results from the Basic Local Alignment Search Tool and a maximum likelihood gene tree generated using PHyML. Two-dimensional DNA barcodes (i.e., QR codes) for the psbK-psbI and psbK-psbI + atpF-atpH regions were created for the validation of fresh or dried R. stricta samples.

show abstract

“…In fact, a large part of the studies on plant barcodes in China are carried out on Chinese medicinal herbs, and the barcodes selected for these studies are often different. For example, Li et al (2014) identified the herbal medicinal materials from Aristolochia using the matK, rbcL, psbA-trnH, and trnL-trnF DNA regions. Guo et al (2017) identified the herbal materials from Cynanchum using the ITS2 barcode; Gong et al (2018) constructed a DNA barcode reference library for "Nan Yao" (crude drugs mainly produced in or imported through tropical and subtropical China, especially the Lingnan region, i.e., the territories south of the Nanling Mountains) using ITS2; and Jiao et al (2018) identified the medicinal Polygonati Rhizoma (a traditional medicinal and edible product with Polygonatum polysaccharides, saponins, phenols, and flavonoids) efficiently and accurately using ITS2 and psbA-trnH sequences.…”

Section: Standard Dna Barcodes For Plant Groups Need Further Explorationmentioning

confidence: 99%

Sixteen Years of DNA Barcoding in China: What Has Been Done? What Can Be Done?

Yang

Zhang

2020

Front. Ecol. Evol.

View full text Add to dashboard Cite

Over the past 16 years, more than half (59.68%) of research papers in China on DNA barcoding have been published in Chinese rather than English. Using the records in the BOLD (Barcode of Life Data) system, we found Chinese scientists have contributed nearly 120,000 DNA barcodes for more than 16,000 species as of September 2019, with barcoded species distributed throughout China. Based on 2,624 articles and 494 dissertations published during the last 16 years, we reviewed the basic statistics of these studies as well as the type of articles contributed by Chinese scientists, the preference of taxonomic groups, the characteristic of barcoding studies in China, the current limitations, and potential future directions as well. We found that most barcode data pertain primarily to plants and animals. Most work in China has focused on verification of the authenticity of species used in traditional Chinese medicine, while other applications have paid more attention to food safety, inspection and quarantine, and the control of pests and invasive species. In methodology and technology, a number of new DNA barcoding methods have been developed by Chinese scientists. However, there are several significant limitations to research into DNA barcoding in China in general, such as the lack of leadership in pioneering international projects, the absence of an open bioinformatics infrastructure, and the fact that some Chinese journals do not clearly require data transparency and availability for DNA barcodes, impeding the further development of barcode libraries and research in China. In the future, Chinese scientists should build authoritative online libraries, while aiming for theoretical innovations for both concepts and methodology of DNA barcoding.

show abstract

Molecular identification and cytotoxicity study of herbal medicinal materials that are confused by Aristolochia herbs

Cited by 16 publications

References 26 publications

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Emergence of Plastidial Intergenic Spacers as Suitable DNA Barcodes for Arid Medicinal Plant <i>Rhazya stricta</i>

Sixteen Years of DNA Barcoding in China: What Has Been Done? What Can Be Done?

Contact Info

Product

Resources

About

Molecular identification and cytotoxicity study of herbal medicinal materials that are confused by Aristolochia herbs

Cited by 16 publications

References 26 publications

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Emergence of Plastidial Intergenic Spacers as Suitable DNA Barcodes for Arid Medicinal Plant &lt;i&gt;Rhazya stricta&lt;/i&gt;

Sixteen Years of DNA Barcoding in China: What Has Been Done? What Can Be Done?

Contact Info

Product

Resources

About

Emergence of Plastidial Intergenic Spacers as Suitable DNA Barcodes for Arid Medicinal Plant <i>Rhazya stricta</i>