Molecular Identification and Detection of Foodborne and Feedborne Mycotoxigenic Fungi

Midorikawa, G. E. O.; Miller, Robert N.; Bittencourt, Daniela

doi:10.1002/9781119374633.ch17

Cited by 8 publications

(2 citation statements)

References 108 publications

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“…Molecular approaches have led to greater confidence and accuracy in the identification of plant pathogenic fungi and oomycetes [39,42,43]. The most prevalent molecular method relies upon polymerase chain reaction (PCR), which has long been used in the field of plant pathology [44][45][46][47].…”

Section: A Brief Overview Of Qpcr For the Detection And Quantificatiomentioning

confidence: 99%

Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA

Kulik

Bilska

Żelechowski

2020

IJMS

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Fungi and oomycetes encompass many pathogens affecting crops worldwide. Their effective control requires screening pathogens across the local and international trade networks along with the monitoring of pathogen inocula in the field. Fundamentals to all of these concerns are their efficient detection, identification, and quantification. The use of molecular markers showed the best promise in the field of plant pathogen diagnostics. However, despite the unquestionable benefits of DNA-based methods, two significant limitations are associated with their use. The first limitation concerns the insufficient level of sensitivity due to the very low and uneven distribution of pathogens in plant material. The second limitation pertains to the inability of widely used diagnostic assays to detect cryptic species. Targeting mtDNA appears to provide a solution to these challenges. Its high copy number in microbial cells makes mtDNA an attractive target for developing highly sensitive assays. In addition, previous studies on different pathogen taxa indicated that mitogenome sequence variation could improve cryptic species delimitation accuracy. This review sheds light on the potential application of mtDNA for pathogen diagnostics. This paper covers a brief description of qPCR and DNA barcoding as two major strategies enabling the diagnostics of plant pathogenic fungi and oomycetes. Both strategies are discussed along with the potential use of mtDNA, including their strengths and weaknesses.

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Section: A Brief Overview Of Qpcr For the Detection And Quantificatiomentioning

confidence: 99%

Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA

Kulik

Bilska

Żelechowski

2020

IJMS

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“…( Kumar et al., 2016 ). Greater confidence, accuracy, specificity, and sensitivity of DNA based molecular techniques ( Capote et al., 2012 ; Midorikawa et al., 2018 ) permit the diagnosis of phytopathogens at primary stages of infection even though they are present at lower DNA concentrations ( Luchi et al., 2013 ; Rollins et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review

Hariharan¹,

Prasannath²

2021

Front. Cell. Infect. Microbiol.

118

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Phytopathogenic fungal species can cause enormous losses in quantity and quality of crop yields and this is a major economic issue in the global agricultural sector. Precise and rapid detection and identification of plant infecting fungi are essential to facilitate effective management of disease. DNA-based methods have become popular methods for accurate plant disease diagnostics. Recent developments in standard and variant polymerase chain reaction (PCR) assays including nested, multiplex, quantitative, bio and magnetic-capture hybridization PCR techniques, post and isothermal amplification methods, DNA and RNA based probe development, and next-generation sequencing provide novel tools in molecular diagnostics in fungal detection and differentiation fields. These molecular based detection techniques are effective in detecting symptomatic and asymptomatic diseases of both culturable and unculturable fungal pathogens in sole and co-infections. Even though the molecular diagnostic approaches have expanded substantially in the recent past, there is a long way to go in the development and application of molecular diagnostics in plant diseases. Molecular techniques used in plant disease diagnostics need to be more reliable, faster, and easier than conventional methods. Now the challenges are with scientists to develop practical techniques to be used for molecular diagnostics of plant diseases. Recent advancement in the improvement and application of molecular methods for diagnosing the widespread and emerging plant pathogenic fungi are discussed in this review.

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Molecular Diagnostics of Soil-Borne and Foliar Diseases of Mulberry: Present Trends and Future Perspective

Gnanesh

Arunakumar

Tejaswi

et al. 2023

Compendium of Plant Genomes

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Molecular Identification and Detection of Foodborne and Feedborne Mycotoxigenic Fungi

Cited by 8 publications

References 108 publications

Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA

Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA

Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review

Molecular Diagnostics of Soil-Borne and Foliar Diseases of Mulberry: Present Trends and Future Perspective

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