Molecular identification and distribution of Anopheles maculipennis complex in the Mediterranean region of Turkey

Şimşek, Fatih Mehmet; Ülger, Celal; Akiner, Mustafa Muhammed; Tuncay, Serap Senol; Kiremit, Ferhat; Bardakçı, Fevzi

doi:10.1016/j.bse.2011.08.010

Cited by 9 publications

(5 citation statements)

References 19 publications

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“…sacharovi Adana population. Less sensitive to insecticide inhibition, changed AChEs have been reported in the Culex genus in many areas (25,(28)(29)(30).…”

Section: Discussionmentioning

confidence: 99%

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Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase

Akıner¹

2014

urkiyeParazitolDerg

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Our results revealed that insecticide resistance against malathion and propoxur increased from 2007 to 2008. Biochemical assay results showed that the AChE insensitivity for 2 test years and insensitive AChE frequency had increased annually. Our results also showed that extensive usage of organophosphate and carbamate for pest control in agriculture is a key factor for malathion and propoxur resistance in all tested populations rather than direct usage of mosquito control.

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“…sacharovi Adana population. Less sensitive to insecticide inhibition, changed AChEs have been reported in the Culex genus in many areas (25,(28)(29)(30).…”

Section: Discussionmentioning

confidence: 99%

“…gambiae, and Cx. tritaeniorhyncus (11,13,(25)(26)(27). Hemingway et al (11) described sensitivity to inhibition to malaoxon and propoxur for the An.…”

Section: Discussionmentioning

confidence: 99%

Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase

Akıner¹

2014

urkiyeParazitolDerg

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“…Sequence analyses reduced probable misidentification of AMC species, due to overlapping egg characters, in different geographic regions. Since then, the species of AMC were identified by ITS2 analyses in many countries: England (Linton et al 2002 ; Danabalan et al 2013 ), Greece (Linton et al 2001 ; Patsoula et al 2007 ), Germany (Proft et al 1999 ; Weitzel et al 2012 ; Kronefeld et al 2012 ; Kronefeld et al 2014 ), Iran (Sedaghat et al 2003 ), Italy (Marinucci et al 1999 ; Di Luca et al 2004 ), Poland (Rydzanicz et al 2017 ), Romania (Nicolescu et al 2004 ) and Turkey (Simsek et al 2011 ), but not in Serbia. Our study applies ITS2 sequence analyses for molecular An.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Anopheles daciae and other Anopheles maculipennis complex species in Serbia

Kavran

Zgomba

Weitzel³

et al. 2018

Parasitol Res

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Malaria is one of the most severe health problems facing the world today. Until the mid-twentieth century, Europe was an endemic area of malaria, with the Balkan countries being heavily infested. Sibling species belonging to the Anopheles maculipennis complex are well-known as effective vectors of Plasmodium in Europe. A vast number of human malaria cases in the past in the former Yugoslavia territory have stressed the significance of An. maculipennis complex species as primary and secondary vectors. Therefore, the present study evaluates the species composition, geographic distribution and abundance of these malaria vector species. Mosquitoes were collected in the northern Serbian province of Vojvodina and analysed by PCR-RFLP, multiplex PCR and sequencing of the ITS2 intron of genomic rDNA. Four sibling species of the An. maculipennis complex were identified. Both larvae and adults of the recently described species An. daciae were identified for the first time in Serbia. In 250 larval samples, 109 (44%) An. messeae, 90 (36%) An. maculipennis s.s., 33 (13%) An. daciae and 18 (7%) An. atroparvus were identified. In adult collections, 81 (47%) An. messeae, 55 (32%) An. daciae, 33 (19%) An. maculipennis s.s., and 3 (2%) An. atroparvus were recorded. The most abundant species in Vojvodina was An. messeae, whereas An. atroparvus was confirmed a rare species in all parts. Since this species is a potentially, highly competent malarial vector, low population density could be crucial to prevent a new establishment of endemic malaria transmission in Serbia.

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“…Significant species-specific polymorphisms in ITS2 PCR product length were identified, demonstrating the suitability of PCR amplification of this region as a diagnostic technique to distinguish between the six or seven anopheline species predominant in Europe (Proft et al 1999;Marinucci et al 1999;Romi et al 2000). Subsequently, this methodology was applied to all presumptive Palaearctic members of the complex, and in some cases, morphological descriptions were integrated to facilitate analysis (Linton et al 2001a(Linton et al , 2002a(Linton et al , b, c, d, 2003Sedaghat et al 2003;Nicolescu et al 2004;Linton et al 2005;Wilke et al 2006;Djadid et al 2007;Bezzhonova and Goryacheva 2008;Simsek et al 2011). Some of these studies led to the discovery of two unrecognised species, Anopheles persiensis Linton, Sedaghat & Harbach (Sedaghat et al 2003) and Anopheles daciae Linton, Nicolescu & Harbach (Nicolescu et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Identification of Anopheles daciae in Germany through ITS2 sequencing

Weitzel¹,

Gauch²,

Becker³

2012

Parasitol Res

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Until the middle of the twentieth century, malaria was frequently endemic in parts of Germany; Anopheles maculipennis complex species were considered the primary vectors. Three species of this complex have been identified in Germany: A. maculipennis s.s., Anopheles messeae and Anopheles atroparvus; the last predominantly from the coastal regions of Northern Germany. Anopheles daciae is a recently described member of the A . maculipennis complex and resembles the well-characterised species A. messeae, although the two species can be distinguished through their egg morphology and sequencing of the internal transcribed spacer 2 (ITS2) region of their nuclear rDNA. In this study, we harvested larval and adult mosquito samples from five breeding sites and ten CO(2) trap collection sites in the Upper Rhine Valley of Southwestern Germany to analyse the complement of anopheline species present. Mosquito ITS2 DNA was extracted and polymerase chain reaction (PCR)-amplified using established protocols. Genomic analysis was performed by a species-diagnostic restriction fragment length polymorphism assay as well as by sequencing of PCR products; the data obtained were aligned against nucleic acid sequences from English mosquitoes retrieved from GenBank. Additionally, the larval breeding sites of A. messeae were characterised through water quality measurement. Forty-seven samples were successfully processed, of which 6 were identified as A. daciae and 41 as A. messeae. All samples of A. daciae, which has not previously been found in Central Europe, originated from one CO(2) trap collection site in Dettenheim, close to Karlsruhe, Southwestern Germany. The identification of this malarial vector in a novel area may have implications for the re-emergence of disease subsequent to climatic changes.

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Molecular identification and distribution of Anopheles maculipennis complex in the Mediterranean region of Turkey

Cited by 9 publications

References 19 publications

Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase

Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase

Distribution of Anopheles daciae and other Anopheles maculipennis complex species in Serbia

Identification of Anopheles daciae in Germany through ITS2 sequencing

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