Molecular Identification and Evaluation of the Genetic Diversity of Dendrobium Species Collected in Southern Vietnam

Abstract

Dendrobium has been widely used not only as ornamental plants but also as food and medicines. The identification and evaluation of the genetic diversity of Dendrobium species support the conservation of genetic resources of endemic Dendrobium species. Uniquely identifying Dendrobium species used as medicines helps avoid misuse of medicinal herbs. However, it is challenging to identify Dendrobium species morphologically during their immature stage. Based on the DNA barcoding method, it is now possible to efficiently identify species in a shorter time. In this study, the genetic diversity of 76 Dendrobium samples from Southern Vietnam was investigated based on the ITS (Internal transcribed spacer), ITS2, matK (Maturase_K), rbcL (ribulose-bisphosphate carboxylase large subunit) and trnH-psbA (the internal space of the gene coding histidine transfer RNA (trnH) and gene coding protein D1, a polypeptide of the photosystem I reaction center (psaB)) regions. The ITS region was found to have the best identification potential. Nineteen out of 24 Dendrobium species were identified based on phylogenetic tree and Indel information of this region. Among these, seven identified species were used as medicinal herbs. The results of this research contributed to the conservation, propagation, and hybridization of indigenous Dendrobium species in Southern Vietnam.

Ethanol Extract of Croton Kongensis Promotes Cell Cycle Arrest and Consequently Inhibits Anchorage-Independent Growth of Cervical Cancer Hela Cells

“…Previous studies showed that diterpenoids extracted from C. kongensis had many biological activities in vitro, including antioxidant, anti-inflammatory, antibacterial, and anticancer [1][2][3][4]. Particularly, the anticancer activity of these diterpenoids had been presented on liver cancer cells and colorectal cancer cells by inducing apoptosis via activating related signaling pathways [9][10][11].…”

“…Many endemic plants of Vietnam have been used as traditional medicines for thousands of years [1][2][3][4].…”

Identification of C‐glycosyl flavones and O‐glycosyl flavones in five Dendrobium species by high‐performance liquid chromatography coupled with electrospray ionization multi‐stage tandem MS

“…Dendrobium, also known as "Shihu", belongs to the orchid family. 1,2 Although seventy-four species and two varieties are known, only about forty species have medicinal value. Furthermore, some of these species with high medicinal values are now endangered.…”

Genetic Diversity and Population Structure of Dendrobium Nobile in Southwest of China Based On Genotyping-by-Sequencing

“…Molecular markers have been developed and a variety of molecular markers have been used to detect genetic diversity and genetic relationships among species in the genus Dendrobium to date, including random-ampli ed polymorphic DNA (RAPD) (Zhu et al 2011), inter simple sequence repeat (ISSR) ), simple sequence repeat (SSR) (Lu et al 2013; Kang et al 2015), sequence related ampli ed polymorphism (SRAP) (Feng et al 2014), inter primer binding repeats site (iPBs) (Cui et al 2020), target region ampli cation polymorphism (trap) (Feng et al 2015), sequence-characterized ampli ed regions (scar) (Zheng et al 2021), these markers are based on electrophoretic map. Other markers are based on sequence differences, including nuclear genome such as ITS, chloroplast genome, including matK, rbcL, psbA-trnH and mitochondrial genome (Ye et al 2017;Wang et al 2018;Nguyen et al 2020). These studies showed that highly genetic differentiation and rich genetic diversity existed among different Dendrobium species.…”