Molecular identification and expression profiles of four splice variants of Sex-lethal gene in Cherax quadricarinatus

Zheng, Jianbo; Cheng, Sun; Jia, Yongyi; Gu, Zhenxin; Li, Fēi; Chi, Meili; Liu, Shili; Jiang, Wenping

doi:10.1016/j.cbpb.2019.05.002

Cited by 39 publications

(29 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CqSxl4 and CqSxl1 were extensively expressed in various tissues while CqSxl2 was almost undetectable. The transcript levels of CqSxl1/3/4 slowly increased along with embryonic development (Zheng et al, 2019). The transcript pattern of Sxl in females and males show that Sxl may not be sex-specific in decapods (Shen et al, 2014;López-Cuadros et al, 2018;Zheng et al, 2019).…”

Section: Sex-lethal (Sxl)mentioning

confidence: 94%

“…In males, the truncated Sxlm has no regulatory effect, leading to the production of an equally inactive default splice variant of Tra (Mullon et al, 2012). In decapods, Sxl has been isolated from Chinese mitten crab (Shen et al, 2014), redclaw crayfish C. quadricarinatus (Zheng et al, 2019), red swamp crayfish P. clarkii (Shi L. et al, 2019), S. verreauxi (Chandler et al, 2015), M. nipponense (Zhang et al, 2013), and P. vannamei (López-Cuadros et al, 2018). Sxl plays crucial roles in sexual differentiation, embryonic development, and gonad development but not in the sex determination of decapods (Shen et al, 2014;López-Cuadros et al, 2018;Zheng et al, 2019).…”

Section: Sex-lethal (Sxl)mentioning

confidence: 99%

“…Recently, several sexual genes have been identified to be involved in the "eyestalk-IAG-testis" endocrine axis (Guo et al, 2019). It has been revealed that some of these genes are regulated by the eyestalk neurohormones and are located at the upstream of IAG and the sexual differentiation cascade (Shen et al, 2014;Ma et al, 2016a;Shi X. et al, 2019;Zheng et al, 2019;Zhong et al, 2019).…”

Section: Regulatory Mechanism Of Sex In Male Decapodsmentioning

confidence: 99%

See 2 more Smart Citations

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

et al. 2021

View full text Add to dashboard Cite

Crustacean culture has been developing rapidly in various parts of the world. Therefore, it is important to understand their reproductive biology. Insulin-like androgenic gland hormone (IAG) secreted from the androgenic gland (AG) is widely accepted as a key regulator of sexual differentiation in male crustaceans. However, recently several sex-related genes (i.e., CFSH, DEAD-box family, Tra-2, Sxl, Dsx, Fem-1, Sox gene family, Foxl2, and Dmrt gene family) have been identified via transcriptomic analysis in crustaceans, indicating that sexual differentiation in crustaceans is more complicated than previously expected. It has been found that several non-coding RNAs (i.e., miRNAs, lncRNAs, and piRNAs) and IAG receptors may be involved in the sexual development of decapods. Identification and study of the regulation mechanism of sex-related genes, non-coding RNAs, and IAG receptors will provide valuable information regarding sexual development in decapods. In this review, the roles of hormonal and genetic factors in both males and females are discussed. In males, crustacean female sex hormone (CFSH), Sxl, Dmrt gene family, Dsx, Sox gene family, GEM, Fem-1, l-GnRH-III, and corazonin play important roles in IAG regulation in the “eyestalk-IAG-testis” endocrine axis. Unlike males, the regulation mechanism and interaction of sexual genes are relatively unknown in females. However, CFSH, IAG, Fem-1, FAMeT, Slo, UCHLs, Erk2, Cdc2, EGFR, Vg, VgR, and VIH seem to play crucial roles during ovarian development. This study summarizes the available information in the field, highlights gaps, and lays the foundations for further studies and a better understanding of the regulatory mechanism of sexual development in decapods.

show abstract

Section: Sex-lethal (Sxl)mentioning

confidence: 94%

Section: Sex-lethal (Sxl)mentioning

confidence: 99%

Section: Regulatory Mechanism Of Sex In Male Decapodsmentioning

confidence: 99%

See 1 more Smart Citation

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

et al. 2021

View full text Add to dashboard Cite

show abstract

“…After being introduced into China in the 1990s, it was favored by domestic breeding enterprises and consumers. Like other crustaceans, the redclaw crayfish exhibited sexual dimorphism in growth traits, with males growing faster and bigger than females at harvest (Zheng et al, 2019). In addition, the female redclaw crayfish holds fewer eggs than other shrimps, which is one of the major factors affecting the development of the industry.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Expression Profiling of Three Transformer-2 Splice Isoforms in the Redclaw Crayfish, Cherax quadricarinatus

et al. 2020

Self Cite

View full text Add to dashboard Cite

Sex determination/sex differentiation is determined by genetics, environmental factors, or the interactions of the two. The Transformer-2 (Tra-2) gene plays an important role in the sex determination cascade signal pathway in insects. In this study, the Tra-2 gene was isolated and characterized from the cDNA library of gonad tissues in the redclaw crayfish, Cherax quadricarinatus. Three splice variants were identified, designated as CqTra-2A, CqTra-2B, and CqTra-2C, and sequence analysis showed that they had a highly conserved RRM domain. Phylogenetic analysis was performed by the NJ method, and the results revealed that the Tra-2 protein of the redclaw crayfish was very closely related to those of Macrobrachium rosenbergii, Fenneropenaeus chinensis, and Macrobrachium nipponense. Real-time PCR analysis showed that the three isoforms were predominantly expressed in the ovary and gradually increased with embryonic development. Additionally, the expression pattern of CqTra-2 at different developmental stages was analyzed by qPCR and revealed that the phase of having a body length of 3 cm may be the key period for the sex differentiation of C. quadricarinatus. RNAi-targeting gene silencing further confirmed the function of CqTra-2 in sexual differentiation in redclaw crayfish. Our experimental data will contribute to understanding the mechanism of sex determination in crustaceans.

show abstract

“…The red claw crayfish Cherax quadricarinatus , a world‐famous freshwater crayfish, has the advantages of large individual size, fast growth speed, high edible proportion, as well as fresh and tender meat (Zheng et al, 2019). However, due to the small size of the red claw crayfish after emergence, its adaptability to the external environment and its ability to resist and avoid enemies is weak (Cheng et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A study on primary diets for juveniles of red claw crayfish Cherax quadricarinatus

Cheng

Wei

Jia

et al. 2020

Aquaculture Research

Self Cite

View full text Add to dashboard Cite

To study the effect of primary diets on the growth of red claw crayfish and provide guidance, three experiments were carried out in this study. The results of Experiment‐I showed: (a) The juveniles with part of yolk sac just disappeared could feed on Artemia larvae, but those with a whole yolk sac could not. The survival and growth rates of the juveniles fed with Artemia were higher than those not fed. (b) There were two eating modes for juveniles. When fed on small food, the juvenile would clamp the food, when fed on large food, the juvenile would hold the food in its arms, then grasped a small piece of food and nibble it. The results of Experiment‐II showed: (a) The survival and growth rates of juveniles fed with Artemia were higher than those fed with the formula feed. (b) The survival rate and body weight were higher when fed twice a day than once a day. The results of Experiment‐III showed: The survival, growth rates, activities of trypsin of group #2 and group #5 were higher than those of other groups. In conclusion, we came to a conclusion that (a) The primary diet should be offered and available in the water environment immediately when the yolk sac was disappearing. (b) Formula feed should be designed to be easily clamped and stable in water. (c) It was necessary to feed the juveniles twice a day. (d) The primary diet with 85% formula feed +14% Artemia +1% spirulina powder could be applied in future seedling production.

show abstract

Molecular identification and expression profiles of four splice variants of Sex-lethal gene in Cherax quadricarinatus

Cited by 39 publications

References 25 publications

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

Molecular Characterization and Expression Profiling of Three Transformer-2 Splice Isoforms in the Redclaw Crayfish, Cherax quadricarinatus

A study on primary diets for juveniles of red claw crayfish Cherax quadricarinatus

Contact Info

Product

Resources

About