Molecular Identification and Genotyping of Atopobium Vaginae, 16S rRNA Gene from Bacterial Vaginosis Miscarriage Women in Al-Hillah City

Abstract

<jats:p>This study was aimed to determine the Atopobium vaginae associated BV in vaginosis women and women with miscarriage. Also other aim, the DNA sequencing was performed for phylogenetic tree analysis of 16SrRNA gene in local Atopobium vaginae isolates in comparison with NCBI-Genbank global Atopobium vaginae isolates and finally submission of the present isolates in NCBI-Genbank database. One hundred fifty (150) high vaginal swabs were collected from women with vaginosis(Seventy five samples were taken from married vaginosis women without miscarriage and Seventy five samples from vaginosis women with miscarriage) from Babylon city hospital and private clinics. The age of patient (15– 45) years. The sample was collected by disposable swabs, genomic DNA was extracted from these swabs. 16s rRNA gene detection by polymerase chain reaction technique . Atopobium vaginae was isolated on Columbia blood agar supplemented with antibiotic for the first time in Iraq, the study confirmed that 9 (12.00%) and 5(6.66%) of Atopobium vaginae out of 150 swabs isolated from miscarriage and non-miscarriage vaginosis women respectively. According to the detection of the 16S rRNA gene, the study revealed that 69(92.00%)and 47(62.66%)of Atopobium vaginae out of 150 swabs obtained from miscarriage and non-miscarriage vaginosis women respectively. BLAST analysis showed that the 16S rRNA gene shared more than 98- 99% sequence compatibility with the sequences of Atopobium vaginae. Furthermore, the phylogenetic tree analysis of the 16S rRNA gene indicated that local Atopobium vaginae (NO.1 and NO. 2 ) isolates shared higher homology with other Atopobium vaginae isolates available in the GenBank. The homology of the nucleotides was between (99.17% and 98.75%) respectively.</jats:p>

Finding a Balance in the Vaginal Microbiome: How Do We Treat and Prevent the Occurrence of Bacterial Vaginosis?

“…Initially isolated as a novel strain in 1999, the facultative anaerobic A. vaginae was recovered from the vagina microflora of a healthy person [142]. Its role in BV has always been the center of the argument until lately, whereby researchers unveiled that the bacterium is indeed an important focal component in the abnormal microflora of BV [143][144][145][146][147][148][149]. As demonstrated through in vitro experiments, A. vaginae triggered the host's innate immune response via vaginal epithelial cells, which later increased the localized production of IL-6 and IL-8 and an antimicrobial β-defensin peptide, mediated through the toll-like receptor 2 [150].…”