Molecular identification and genotyping of Acinetobacter baumannii isolated from burn patients by PCR and ERIC-PCR

Abstract

Background: Acinetobacter baumannii is one of the most important agents of hospital infections. Rapid and accurate identification and genotyping of A. baumannii is very important, especially in burn hospitals in order to prevent the spread of related nosocomial infections and to further epidemiological studies. Material and methods: For two months, 82 A. baumannii isolates were collected from burn wound swabs of patients in a major burn hospital in Tehran. A. baumannii isolates were identified by conventional microbiological test and polymerase chain reaction (PCR) using the primers of blaOXA-51 gene, while the genetic linkage of A. baumannii isolates was investigated by enterobacterial repetitive intragenic consensus (ERIC)-PCR technique. Similarity, a cut-off of ⩾ 95% was considered for classifying the genotypes. Results: The molecular test (PCR) confirmed 97.56% of phenotypic results for the detection of A. baumannii isolates. ERIC-PCR results revealed 14 different ERIC patterns (ERIC-types) including 11 common types and three unique types. Conclusion: Our findings show that we can simply and quickly detect A. baumannii isolates by PCR using blaOXA genes and genetic diversity by ERIC-PCR, respectively. These rapid and simple techniques for the routine screening and identification of clinical A. baumannii isolates could be useful with epidemic potential.

Detection of NDM and OXA-48 Resistant Genes in Acinetobacter Baumannii Isolated FromIntensive Care Units’ Patients Clinical Samples in Khartoum State

“…In term of molecular con rmation, the frequency of A. baumannii was found in the present study was 47 (94%) which is similar to the frequency rate found out by Falah etal., in 2019 whom con rmed 80 (97.56%) of A. baumannii isolates in a total of 82 [2]. While Marathe et al, in 2019 found results which were strongly disagreed with the frequency found in the present study they examined a total of 112 sample and found only 33(30%) con rmed as A. baumannii [10].…”

“…Four species of Acinetobacter including A. calcoaceticus, A. baumannii, A. pittii and A. nosocomialis are similar to each other and it is di cult to distinguish them by phenotypic characteristics. A. baumannii is the commonest species isolated from human clinical specimens, followed by such species as A. luffy, A. pittii, A. nosocomialis, A. haemolyticus and A. johnsonii [2,3].…”

Molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Hamadan, west of Iran

“…In the study by Fallah et al, 82 samples of A. baumannii were isolated from the burn hospital and divided into 14 types of ERIC-PCR patterns, including 11 common types and 3 unique types. In this study, 77 samples were classified into 9 main genotypes [ 24 ]. On the other hand, in the current study 14 different ERIC-types including 7 common types and 7 unique or single types (Strains No.…”

Prevalence of OXA-Type β-Lactamase Genes among Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Thailand

“…Genomic DNA from Acinetobacter species was prepared using the boiling method [41]. Quantification of the extracted DNA was determined by spectroscopy at 260 nm.…”