Molecular Identification and Growth Inhibition of Some Human Pathogenic Bacteria Isolated From King Fahad General Hospital, Jeddah

Abstract

Antimicrobial properties of bacterial antagonist against human pathogenic bacteria have become a field of increasing importance in the medical sector. The present study has been carried out to identify the antimicrobial properties of actinomycetes bacteria against five human pathogenic bacteria viz., Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli isolated from many wound swabs, from different service units of King Fahad General Hospital (KFGH), Jeddah. These isolated bacterial isolates were identified by using morphological and physiological characters along with molecular techniques. The antibacterial activities of five actinomycetes species along with Ampicillin (5µg/ml) as positive control were also determined against the multidrug resistant S. aureus, S. pyogenes, P. aeruginosa, A. baumannii and E. coli by using disc diffusion assay. Result of study revealed that among the tested five actinomycetes species, Streptomyces 5 (St 5) was highly effective against P. aeruginosa, S. aureus, S. pyogenes and E. coli while it showed weak activity against A. baumannii. As compared to this, Streptomyces 2, 3 and 4 showed moderate antibacterial activities while Streptomyces 1 showed the lowest activity. Further, all the tested Streptomyces extracts and Ampicillin were found weak against the multi-drug resistant A. baumannii. In conclusion, actinomycetes especially genus Streptomyces can be used as a safe and effective source against multidrug resistant bacteria.