Molecular identification and isolation of the Waxy locus in maize

Shure, Mavis; Wessler, Susan R.; Fedoroff, Nina V.

doi:10.1016/0092-8674(83)90225-8

Cited by 774 publications

(383 citation statements)

References 29 publications

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“…Depending on colony size, one-quarter to onehalf of the colony was used for DNA extraction; the remainder was dried as a voucher specimen. Samples were ground in liquid nitrogen with a disposable plastic pestle, and total DNA was extracted in urea buffer by the method of Shure et al (1986). DNA pellets were resuspended in 100 lL water containing 20 lg/mL RNase A.…”

Section: Molecular Methodsmentioning

confidence: 99%

Molecular phylogeny and phylogeography of free-living Bryozoa (Cupuladriidae) from both sides of the Isthmus of Panama

Dick

Herrera-Cubilla

Jackson

2003

Molecular Phylogenetics and Evolution

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Section: Molecular Methodsmentioning

confidence: 99%

Molecular phylogeny and phylogeography of free-living Bryozoa (Cupuladriidae) from both sides of the Isthmus of Panama

Dick

Herrera-Cubilla

Jackson

2003

Molecular Phylogenetics and Evolution

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“…Southern Hybridisation and PCR DNA was isolated from in vitro grown plants according to the method of Shure et al (1983).…”

Section: Potato Transformation and Regenerationmentioning

confidence: 99%

Generation of Marker- and Backbone-Free Transgenic Potatoes by Site-Specific Recombination and a Bi-Functional Marker Gene in a Non-Regular One-Border Agrobacterium Transformation Vector

2006

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A binary vector, designated PROG-MO, was constructed to assess the potential of the Zygosaccharomyces rouxii R/Rs recombination system for generating marker-and backbone-free transgenic potato (Solanum tuberosum) plants with high transgene expression and low copy number insertion. The PROGMO vector utilises a constitutively expressed plant-adapted R recombinase and a codA-nptII bi-functional, positive/negative selectable marker gene. It carries only the right border (RB) of T-DNA and consequently the whole plasmid will be inserted as one long T-DNA into the plant genome. The recognition sites (Rs) are located at such positions that recombinase enzyme activity will recombine and delete both the bi-functional marker genes as well as the backbone of the binary vector, leaving only the gene of interest flanked by a copy of Rs and RB. Efficiency of PROGMO transformation was tested by introduction of the GUS reporter gene into potato. It was shown that after 21 days of positive selection and using 300 mgl -1 5-fluorocytosine for negative selection, 29% of regenerated shoots carried only the GUS gene flanked by a copy of Rs and RB. The PROGMO vector approach is simple and might be widely applicable for the production of marker-and backbone-free transgenic plants of many crop species.

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“…Association and selection tests of the maize orthologue dwarf8 also suggest that this locus is currently a target of selection and adaptation of maize to various flowering times (Thornsberry et al, submitted). Orthologous loci have also been targeted for grain processing ; for example, low amylose was selected for in both rice and maize by using the starch synthase-encoding waxy loci (Isshiki et al, 1998 ;Shure et al, 1983).…”

Section: What Genes Ha E Been the Targets Of Domestication And Breeding?mentioning

confidence: 99%

Molecular Diversity, Structure and Domestication of Grasses

2001

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Molecular identification and isolation of the Waxy locus in maize

Cited by 774 publications

References 29 publications

Molecular phylogeny and phylogeography of free-living Bryozoa (Cupuladriidae) from both sides of the Isthmus of Panama

Molecular phylogeny and phylogeography of free-living Bryozoa (Cupuladriidae) from both sides of the Isthmus of Panama

Generation of Marker- and Backbone-Free Transgenic Potatoes by Site-Specific Recombination and a Bi-Functional Marker Gene in a Non-Regular One-Border Agrobacterium Transformation Vector

Molecular Diversity, Structure and Domestication of Grasses

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